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Double-labeled nano time-resolved fluorescence immunochromatographic quantitative test paper for mycoplasma pneumoniae antibodies and preparation method of test paper

A time-resolved fluorescence and mycoplasma pneumoniae technology, which is applied in the field of immunoassay, can solve the problems of high instrument requirements, time-consuming, complicated operation, etc., and achieve the effects of high sensitivity, high accuracy, and convenient clinical use

Inactive Publication Date: 2016-10-26
WUXI PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it takes about 3 hours for TRFIA to complete a test, and other equipment such as a plate washer is required. It is generally suitable for large-scale testing, and there are disadvantages such as complex operation, time-consuming, and high-level instrument requirements for single-unit determination.

Method used

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  • Double-labeled nano time-resolved fluorescence immunochromatographic quantitative test paper for mycoplasma pneumoniae antibodies and preparation method of test paper
  • Double-labeled nano time-resolved fluorescence immunochromatographic quantitative test paper for mycoplasma pneumoniae antibodies and preparation method of test paper
  • Double-labeled nano time-resolved fluorescence immunochromatographic quantitative test paper for mycoplasma pneumoniae antibodies and preparation method of test paper

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Embodiment 1

[0040] A mycoplasma pneumoniae antibody double-labeled nanometer time-resolved fluorescent immunochromatographic quantitative test paper, including a sticky bottom layer 1, a sample pad 2, a binding pad 3, a nitrocellulose membrane 4 and an absorbent paper 5;

[0041] A sample pad 2, a binding pad 3, a nitrocellulose membrane 4, and an absorbent paper 5 are sequentially arranged on the sticky bottom layer 1; wherein the nitrocellulose membrane 4 is directly arranged on the sticky bottom layer 1, and the rear end of the binding pad 3 is lapped in nitric acid On the front end of the cellulose membrane 4, the rear end of the sample pad 2 is lapped on the front end of the bonding pad 3, and the front end of the sample pad 2 is directly bonded to the adhesive bottom layer 1; the front end of the absorbent paper 5 is lapped on the nitrocellulose On the rear end of the membrane 4; wherein the nitrocellulose membrane 4 is provided with a detection band T and a quality control band C. ...

Embodiment 2

[0051] The preparation method of mycoplasma pneumoniae antibody double-labeled nanometer time-resolved fluorescent immunochromatography quantitative test strip comprises the following steps:

[0052] (1) Preparation of raw materials: prepare the required adhesive bottom layer 1, sample pad 2, binding pad 3, nitrocellulose membrane 4 and absorbent paper 5;

[0053] (2) Antibody pretreatment: use 0.05mol / L, pH 7.2-7.6 phosphate buffer at 4°C to dialyze mouse anti-human IgG antibody and mouse anti-human IgM antibody with a 10kDa membrane, overnight, to obtain the pretreatment Antibody after treatment;

[0054] (3) Preparation of nanospheres coated with europium ions labeled with mouse anti-human IgG antibody: take the nanospheres coated with europium ions, and use distilled water to prepare a nanosphere solution with a mass concentration of 1%; take 250 μL of the nanosphere solution , adding carbodiimide and step (2) pretreated mouse anti-human IgG antibody wherein, the final co...

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Abstract

The invention discloses double-labeled nano time-resolved fluorescence immunochromatographic test paper for mycoplasma pneumoniae antibodies and a preparation method of the test paper. The test paper comprises a viscous sublayer, a sample pad, a binding pad, a nitrocellulose membrane and water absorbing paper; detection tapes for the mycoplasma pneumoniae antibodies and quality control tapes for rabbit-anti-rat polyclonal antibodies are sequentially arranged on the nitrocellulose membrane at intervals, and nanoparticles which label mouse-anti-human IgG antibodies and wrap europium ions and nanoparticles which label mouse-anti-human IgM antibodies and wrap samarium ions are sprayed on the surface of the binding pad. According to the test paper, quantitative detection on mycoplasma pneumoniae IgG antibodies and mycoplasma pneumoniae IgM antibodies can be performed on one piece of time-resolved fluorescence immunochromatographic test paper, high accuracy of a detection result and a small error of the mycoplasma pneumoniae IgG antibodies and the mycoplasma pneumoniae IgM antibodies in the same sample liquid can be guaranteed, and great convenience is provided for clinic use.

Description

technical field [0001] The invention relates to a double-labeled nanometer time-resolved fluorescent immunochromatography test paper for mycoplasma pneumoniae antibody and a preparation method thereof, belonging to the technical field of immune detection. Background technique [0002] Mycoplasma pneumonia (MP) is a pneumonia caused by the adhesion of Mycoplasma pneumoniae (MP) to the neuraminic acid receptors of the host respiratory mucosal epithelial cells and the release of toxic metabolites. The onset of MP is slow, with fever, paroxysmal irritating cough, a small amount of mucus or mucopurulent sputum (occasionally with bloody sputum), and the pulmonary signs are often not obvious, but it is easy to cause involvement of multiple extrapulmonary systems, and can also be life-threatening or serious. die. MP is more likely to occur in children or adolescents. Serological epidemiological studies have shown that 20.7%-38.9% of patients with community-acquired pneumonia (CAP) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56933G01N2333/30G01N2800/12
Inventor 胡志刚刘洁钱俊高宏杨雪俞蕾叶燕
Owner WUXI PEOPLES HOSPITAL