Identification method of red silk thread

A technology of red silk thread and contrasting medicinal materials, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve satisfactory chromatographic effects, less interference from impurities, and satisfactory separation

Active Publication Date: 2018-01-05
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, how to quickly distinguish the true and false of red silk thread, there is no effective method at present, and the true and false of red silk thread directly determines its medicinal effect

Method used

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  • Identification method of red silk thread
  • Identification method of red silk thread
  • Identification method of red silk thread

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Select the red silk thread with batch number 151201 as a sample.

[0022] 2. Take 2g of sample powder and place it in a stoppered conical flask, then add 25ml of anhydrous methanol solution to the conical flask, ultrasonically treat it for 30 minutes, filter, evaporate the filtrate to dryness, and dissolve the residue with 1.5ml of absolute ethanol. Incline the supernatant, pass through a neutral alumina column (200-300 mesh, 1 g, 1 cm in diameter), and collect the column liquid as the test solution.

[0023] 3. Take another 2 g of red silk thread reference medicinal material (provided by the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine), and make a reference medicinal material solution according to the method in step 2.

[0024] 4. Take the β-sitosterol reference substance and add it to absolute ethanol to make a solution containing 0.5 mg β-sitosterol per 1 ml of ethanol as the reference substance solution.

[0025] 5. Take 5 μ...

Embodiment 2

[0027] 1. Select the red silk thread with batch number 151101 as a sample.

[0028] 2. Take 2g of sample powder, put it in a conical flask with a stopper, add 25ml of absolute ethanol solution to the conical flask, treat it ultrasonically for 30 minutes, filter, evaporate the filtrate to dryness, and dissolve the residue with 1.5ml of absolute ethanol. Incline the supernatant, pass through a neutral alumina column (200-300 mesh, 1 g, 1 cm in diameter), and collect the column liquid as the test solution.

[0029] 3. Take another 2 g of red silk thread reference medicinal material (provided by the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine), and make a reference medicinal material solution according to the method in step 2.

[0030] 4. Take the β-sitosterol reference substance and add it to absolute ethanol to make a solution containing 0.5 mg β-sitosterol per 1 ml of ethanol as the reference substance solution.

[0031] 5. Take 5 μl each ...

Embodiment 3

[0033] 1. Select the red silk thread with batch number 19315812 as a sample;

[0034] 2. Take 2g of sample powder, put it in a conical flask with a stopper, add 25ml of ethanol solution with a volume concentration of 60% to the conical flask, reflux for 1 hour, filter, evaporate the filtrate to dryness, and wash the residue with absolute ethanol 1.5ml was dissolved, and the supernatant was poured, passed through a neutral alumina column (200-300 mesh, 1g, 1cm in diameter), and the column liquid was collected as the test solution.

[0035] 3. Take another 2 g of red silk thread reference medicinal material (provided by the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine), and make a reference medicinal material solution according to the method in step 2.

[0036] 4. Take the β-sitosterol reference substance and add it to absolute ethanol to make a solution containing 0.5 mg β-sitosterol per 1 ml of ethanol as the reference substance solution. ...

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Abstract

The invention provides a Lycianthes biflora identification method. The method comprises 1, taking 2g of sample powder, putting the sample powder into a conical flask with a plug, adding 25ml of an ethanol solution with a volume concentration of 60% into the conical flask, carrying out ultrasonic treatment for 30min, filtering the solution, evaporating the filtrate, dissolving the residues through 1.5ml of anhydrous ethanol, taking the supernatant, treating the supernatant through a neutral alumina column and collecting the treated supernatant as a sample solution, 2, taking 2g of a Lycianthes biflora contrast medicinal material and preparing a contrast medicinal material solution through the process 2, 3, adding a beta-sitosterol contrast substance into anhydrous ethanol to obtain a beta-sitosterol solution as a contrast solution, wherein 1ml of ethanol in the beta-sitosterol solution contains 0.5mg of beta-sitosterol, and 4, sucking 5 microliters of the sample solution, 5 microliters of the contrast medicinal material solution and 2 microliters of the contrast solution, respectively dispensing the solutions to the same silica gel G thin layer plate, developing the solutions through cyclohexane-ether-ethyl acetate (15: 4: 3) as a developing agent, taking out the thin layer plate, carrying out air drying, spraying an ethanol sulfate solution with a volume concentration of 10% to the thin layer plate, and heating the thin layer plate at a temperature of 105 DEG C until the spots produce clear colors, wherein in a test sample chromatogram, positions corresponding to the contrast medicinal material chromatogram and contrast substance chromatogram produce same color spots.

Description

technical field [0001] The invention relates to a method for identifying red silk thread medicinal materials, in particular to a method for identifying red silk thread which is convenient for judging the quality and authenticity of red silk thread. Background technique [0002] The red thread is Peristrophe baphica (Spreng) Bremek. Also known as red silk grass, silk thread grass, dyed nine-headed lion grass, mountain blue, etc. The whole herb is used as medicine, and its nature and flavor are sweet, light and slightly cold. Efficacy Clearing the lung and relieving cough, dissipating blood stasis and stopping bleeding. It is used for cough due to lung heat, hemoptysis and hematemesis due to internal injury, external application for bruises and bruises, etc. Red silk thread has a long history of use in Guangdong, with definite curative effects. It was included in the second volume of "Guangdong Traditional Chinese Medicine" in 1996. [0003] At present, how to quickly dist...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 冼绍祥
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU UNIV OF CHINESE MEDICINE
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