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Paenibacillus polymyxa and application thereof

A polymyx spore-like and bacillus technology is applied in the field of biological control of plant diseases, which can solve the problems of large amount of chemical use, many times, and pesticide residues.

Inactive Publication Date: 2016-11-09
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the large amount and frequent use of chemical agents for the prevention and control of vegetable diseases, which are likely to cause pesticide residues, the use of chemical agents should be reduced and biological control measures should be increased

Method used

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  • Paenibacillus polymyxa and application thereof
  • Paenibacillus polymyxa and application thereof
  • Paenibacillus polymyxa and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: strain isolation and identification

[0015] Strain isolation and antagonistic determination: (1) Take 1g of soil samples from different regions, add water to make a soil suspension with a certain concentration, and then perform gradient dilution for 10 3 ~10 5 times, take 100ul of the diluted solution to smear the plate, and place it in a 28-degree incubator for 48 hours. (2) Pick a single colony and save it. (2) With Botrytis cinerea as the target fungus, use the confrontation culture method to measure the antagonism of the isolated strains, culture at 25 degrees for 4-5 days, and measure the size of the antagonism circle. (3) For the strains with better antagonistic effect, the antagonistic spectrum was determined by the same method. (4) Subculture the strain with stronger antagonistic effect in beef extract peptone culture solution for ten generations, and then test the stability of the antagonistic effect of the strain by confrontation culture.

...

Embodiment 2

[0018] Embodiment 2: field test

[0019] (1) The test strain JY1-5 was shaken and cultured with beef extract peptone culture solution for 5-7 days to obtain a bacterial suspension.

[0020] (2) Select the tomato growing field where the fruit has been diseased, remove all the diseased fruit, spray the bacterial liquid on the fruit surface, and use Sukeling (Hebei Cangzhou Zhongtian Chemical Co., Ltd.), a commonly used agent for preventing and treating tomato gray mold, as the chemical agent Contrast, spray clear water is uncontrollable control, spray once every 7 days. 4m per cell 2 ,repeat three times.

[0021] In the experiment on the control of Botrytis cinerea, the disease index of the control was 36.57%, 30.09% of saccharin, and 23.61% of JY1-5; the incidence rate of the control was 37.56%, 33.43% of saccharin, was 23.61%. Among them, the control effect of Sukeling on Botrytis cinerea was 17.73%, and the control effect of JY1-5 was 40.59%. It can be seen that the disea...

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Abstract

The invention relates to biological prevention and control of plant diseases, belongs to the fields of biological prevention and control research and application in a plant protection subject and discloses paenibacillus polymyxa JY1-5 resisting a plurality of plant pathogenic fungi. The number of the paenibacillus polymyxa preservation is CGMCC No. 12410. The paenibacillus polymyxa can effectively restrain vegetable botrytis cinerea, the effect of preventing and controlling botrytis cinerea is superior to that of common chemical agent procymidone, compared with the prevention efficiency of procymidone, the prevention efficiency of the paenibacillus polymyxa is improved by 57.3%, and the paenibacillus polymyxa has potential of preventing and controlling the multiple plant diseases. Increasing of biological agent using and decreasing of chemical agent using are beneficial to environment protection, pesticide residue reduction and agricultural sustainable development.

Description

technical field [0001] The invention relates to biological control of plant diseases and belongs to the field of biological control research and application in the discipline of plant protection. Background technique [0002] Plant disease is one of the main obstacles restricting the safe growth of plants. The current prevention and control method is mainly spraying chemical agents. Vegetable crops have a short growth period, and the frequent use of chemical agents has the risk of pesticide residues. The development and use of biological pesticides can reduce the amount of chemical agents used. Microorganisms that have antagonistic effects on plant pathogenic bacteria are the main source for the development of biopesticides. Such microorganisms are diverse and widely distributed. Even if the same microorganism is distributed in different regions, the strength or mechanism of the antagonistic effect is also different. To mine biocontrol microbial resources, the first step is...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/12
CPCA01N63/00C12N1/205C12R2001/12
Inventor 张清霞童蕴慧何玲玲陈夕军
Owner YANGZHOU UNIV
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