Unlock instant, AI-driven research and patent intelligence for your innovation.

Probiotic polyclone fermentation medium and preparation method thereof

A technology of probiotics and culture medium, applied in the field of bioengineering, can solve the problems of high requirements for equipment conditions, turbid physical properties, complex production processes, etc., and achieves reduction of time and raw material costs, low requirements for equipment conditions, and simple and clear chemical composition. Effect

Pending Publication Date: 2016-11-09
GUIZHOU MEDICAL UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional probiotic medium and its fermentation technology or process have the following disadvantages: 1. The chemical composition of the medium is complex, the physical properties are turbid, the number of bacteria in the culture is small, and it can only be used for single-clonal fermentation
2. The traditional fermentation technology requires high equipment conditions, complex production process, difficult to control the production process and product quality

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Probiotic polyclone fermentation medium and preparation method thereof
  • Probiotic polyclone fermentation medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh the following ingredients according to the amount, 5g peptone, 3g glucose, 3g yeast powder, 1g inulin, 1g fructose, 1g agar, 0.1g dipotassium hydrogen phosphate, 0.1g calcium gluconate, 0.05g zinc gluconate, 0.1 g ferric citrate , 1000mL of water. Put it in a container to make a solution for later use; add an appropriate amount of sodium bicarbonate to the solution to adjust the pH value of the solution until the pH value of the solution is 7.6 to 7.8; then divide the solution, and then perform high-pressure steam sterilization and control the temperature Prepare liquid culture medium at 121.3°C for 15-30 minutes.

Embodiment 2

[0017] Weigh the following ingredients according to the amount, 5g peptone, 3g glucose, 3g yeast powder, 1g inulin, 1g fructose, 8g agar, 0.1g dipotassium hydrogen phosphate, 0.1g calcium gluconate, 0.05g zinc gluconate, 0.1 g ferric citrate , 1000mL of water. Put it in a container to make a solution for later use; add an appropriate amount of sodium bicarbonate to the solution to adjust the pH value of the solution until the pH value of the solution is 7.6 to 7.8; then divide the solution, and then perform high-pressure steam sterilization and control the temperature At 121.3°C for 15-30 minutes, a solid medium was prepared.

[0018] Table 1 is a comparison of the basic features of the traditional fermentation method using this medium and the polyclonal fermentation method:

[0019]

[0020]

[0021] It can be seen from the above table that the culture medium prepared by the above steps contains essential, definite and safe nutrients, chemical substances and organic mi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of bioengineering, in particular to a probiotic polyclone fermentation medium and a preparation method thereof. The medium is prepared from, by mass, 5 g of peptone, 3 g of glucose, 3 g of yeast powder, 1 g of inulin, 1 g of fructose, 1-8 g of agar, 0.1 g of dipotassium phosphate, 0.1 g of calcium gluconate, 0.05 g of zinc gluconate, 0.1 g of ferric citrate and 1,000 mL of water. The invention further discloses the preparation method of the probiotic polyclone fermentation medium. The probiotic polyclone fermentation medium and the preparation method thereof have the advantages that a large number of bacterial cultures are obtained, the method is suitable for polyclone fermentation of probiotics, the requirement for equipment conditions is low, the yield of probiotics is high, and the production process and product quality are easy to control.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a culture medium for polyclonal fermentation of probiotics and a preparation method thereof. Background technique [0002] Microbial fermentation technology or microbial fermentation engineering is a technical method for industrialized artificial cultivation of microorganisms using medium and fermentation technology to obtain a large number of microbial cells or / and microbial active metabolites. Therefore, microbial fermentation engineering mainly includes microbial strains, culture medium, fermentation technology, separation and extraction of metabolites and other links. Culture medium is a nutrient matrix used to cultivate microorganisms, and its quality directly affects the growth, reproduction and metabolic activities of microbial strains, as well as the nature and quantity of metabolites. Fermentation technology is a technical method to provide external conditions su...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12R1/01C12R1/225C12R1/46
CPCC12N1/20
Inventor 王和王丹霓
Owner GUIZHOU MEDICAL UNIV