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Method for preparing microbial fertilizer promoting morchella growth by spent mushroom compost of lentinus edodes and application

A technology of microbial fertilizer and shiitake mushroom chaff, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of large differences in nutrients, not targeting edible fungi, and single bacterial agent, etc., and achieve soil fertility. Improve and protect the health of morel mushrooms, the effect of stronger bacteria and stronger bacteria

Inactive Publication Date: 2018-08-24
LIAONING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned hickory chick culture technology mainly has the following disadvantages: (1) bacteria chaff is various, and nutrient difference is bigger, some crude protein ≥ 8%, crude fiber content ≤ 25%, another kind of crude protein 25%, using a kind of chaff shiitake mushroom to overcome the disadvantages of nutrient content differences; (2) the application of bacterial agents is single, or several are mainly around food crops, vegetable crops, and flower crops, and are not aimed at edible fungi, especially morels Microbial fertilizer required for production

Method used

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  • Method for preparing microbial fertilizer promoting morchella growth by spent mushroom compost of lentinus edodes and application
  • Method for preparing microbial fertilizer promoting morchella growth by spent mushroom compost of lentinus edodes and application
  • Method for preparing microbial fertilizer promoting morchella growth by spent mushroom compost of lentinus edodes and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 composite microbial preparation

[0029] Composite microbial preparations include the following microorganisms: Bacillus megaterium, Bacillus subtilis, Bacillus mucilaginosus, Saccharomyces cerevisiace, Streptomyces jingyangensis, Aspergillus ornatus and Photosynthetic Bacteria are Rhodopseudomonas palustris.

[0030] The preparation method of composite microbial preparation comprises the cultivation and mixing of bacterial classification, and its steps are as follows:

[0031] 1) Cultivation of Bacillus subtilis

[0032] Agar nutrient medium: beef extract 0.3g, NaCl 0.5g, peptone 10g, agar 2g, distilled water 100ml, pH natural;

[0033] Liquid fermentation medium: 1g of maltose, 1g of wheat bran, 1g of tryptone, 1.5g of soybean meal powder, 0.1g of yeast extract, 0.25g of K2HPO, 0.1g of KH2PO, 0.15g of Tween 40, sterilized at 121°C for 30 minutes.

[0034] According to the routine operation in the field of microbiology:

[0035] Activation → primary am...

Embodiment 2

[0067] Embodiment 2 A kind of method that utilizes mushroom chaff to prepare the microbial fertilizer that promotes the growth of hickory chick

[0068] Microbial organic fertilizer production process: fermentation is divided into two steps, namely pre-fermentation and post-fermentation. Pre-fermentation is the process of fresh organic fertilizers being carried out in special fermentation bins or fermentation tanks under aerobic conditions and the action of microorganisms; post-fermentation is to accumulate fermented finished products so that the organic substances that have not been completely decomposed continue to decompose and become stable. humus substances.

[0069] Raw material ratio: 50 parts of mushroom chaff, 25 parts of cow dung, 8 parts of humic acid, 3 parts of soybean meal, 3 parts of amino acid, 3 parts of medium and trace elements (superphosphate, MgSO 4 , ZnSO 4 , FeSO 4 , MnSO 4 、CuSO 4 , alginic acid, chitin) as the raw material carrier, adjust the prop...

Embodiment 3

[0075] Embodiment 3: the application method of microbial fertilizer:

[0076] 1) Before sowing, at first the soil is filled with water, so that the 0-40CM deep soil reaches more than 30% of saturated water content; through machine plowing, 500 kg / mu of microbial fertilizer prepared in soil application embodiment 2; 20 days before sowing, the soil containing microbial fertilizers will be plowed again, so that the microbial fertilizers can fully activate the beneficial living bacteria in the soil, and at the same time improve the soil fertility, so as to provide a good growth environment for morels, a saprophytic bacterium, to inhibit miscellaneous bacteria and promote Morel growth targets for increased yield and quality.

[0077] The application effect of the microbial fertilizer in the artificial cultivation of morels can promote the production of morels, has the effect of anti-miscellaneous bacteria pollution, the effect of increasing production can increase the yield by 25-5...

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Abstract

The invention discloses a method for preparing a microbial fertilizer promoting morchella growth by spent mushroom compost of lentinus edodes. The microbial fertilizer comprises the spent mushroom compost of lentinus edodes, cow dung, humic acid, bean pulp, amino acid, medium trace elements and compound microorganism preparations. Fermentation is performed by two steps, primary fermentation includes that fresh organic fertilizers are fermented in a special fermentation bin GALLS or fermentation tank under aerobic condition and under the action of microorganisms. Secondary fermentation includesthat fermented finished products are stacked, the compound microorganism preparations are added to continue decomposing incompletely decomposed organic substances, the organic substances are changedinto stable humic substances, soil beneficial viable bacteria can be sufficiently activated by the microbial fertilizer, soil fertility is improved, good growth environments are provided for morchellaserving as autochthonic saprophytic bacteria, infectious microbes are inhibited, morchella growth is promoted, and yield and quality are improved. The method is a beneficial way for turning waste into wealth, resources of the spent mushroom compost of lentinus edodes are huge in China, environmental problems caused by stacking of the spent mushroom compost of lentinus edodes result in pollution,and the problems of bacteria-forestry contradiction and mushroom residue pollution can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of microbial resource application, and in particular relates to a method for preparing microbial fertilizer for promoting the growth of hickory chick by using shiitake mushroom chaff. Background technique [0002] Morchella spp. taxonomically belongs to Ascomycota, Pezizomycetes, Pezizales, and Morcellaceae. Wild morels are mainly distributed in Yunnan, Sichuan, Qinghai, Henan, Shaanxi, Gansu, Tibet, Xinjiang, Shanxi, Jilin, Liaoning, Jiangsu and other regions in China. Most of the morels are delicious edible fungi, ranking first among the four famous wild fungi in the world. As early as the 1880s, research on the artificial cultivation of morels was widely carried out in Britain, the United States, France, Germany and other countries. Cultivation has experienced the transformation of propagation promotion technology, bionic cultivation technology, mycorrhizal cultivation technology, semi-artificial cultiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C05G3/04C05F17/00A01C21/00C12N1/20C12N1/16C12N1/14C12R1/11C12R1/125C12R1/07C12R1/865C12R1/465C12R1/69C12R1/38C05G3/80
CPCA01C21/005C05B1/02C05F17/50C05G3/80C12N1/14C12N1/16C12N1/20C05C11/00C05D5/00C05D9/02C05F3/00C05F5/00C05F5/002C05F11/00C05F11/02C05F11/08Y02W30/40
Inventor 张季军肖千明张敏李红刘娜王秀兰袁靖冯侠
Owner LIAONING ACAD OF AGRI SCI
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