A method for preparing microalgae
plant-based yogurt by means of using acid- and
aroma-producing microbes. The method comprises: S1, selection of microalgae raw materials: using microalgae with a
protein content of ≥35% in
algae cells as raw materials; S2,
cell disruption: disrupting the
algae cells by means of using a high-pressure
homogenizer to obtain a wall-broken algal
slurry; S3, blending and sterilization: adding
sugar, a
thickening agent and
purified water to the wall-broken algal
slurry, adjusting the
protein concentration of the wall-broken algal
slurry to obtain a blended algal slurry, and performing sterilization treatment; S4,
fermentation: inoculating a mixed strain for two-stage
fermentation, comprising: first inoculating
Clostridium butyricum,
Clostridium kluyveri and
yeast for a predetermined period of time, and then inoculating
lactic acid bacteria for
fermentation; and S5, sub-packaging and
refrigeration: sub-packaging the fermented algal slurry into a packaging container, placing same at 0°C-8°C for
refrigeration and post-
ripening, and thus obtaining the microalgae
plant-based yogurt. By means of fermentation with
clostridia and
yeast, most of algal off-
flavor substances are metabolized, and a richer variety of aromatic substances are produced compared with fermentation with
lactic acid bacteria alone, resulting in an aromatic and palatable microalgae-based yogurt that meets the needs of populations with milk allergies and those pursuing
plant-based foods.