Novel method for extracting fulvic acids from industrially-produced waste edible fungi residues by fermentation

A technology of edible fungi and fulvic acid, applied in the biological field, can solve the problems of multiplication of bacteria, threats to the edible fungus industry, waste of organic resources, etc., and achieve the effect of creating economic value

Inactive Publication Date: 2016-02-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the industrialized production of edible fungus residues in my country is huge, but how to deal with the residues in an environmentally friendly and effective manner has not been well resolved; a large number of residues and waste sticks are piled up on the spot every y

Method used

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  • Novel method for extracting fulvic acids from industrially-produced waste edible fungi residues by fermentation
  • Novel method for extracting fulvic acids from industrially-produced waste edible fungi residues by fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0033] 1 strain

[0034] Bacillus subtilis (Bacillus subtilis) is a strain numbered CGMCC1.210 of the China Microorganism Culture Collection Management Center; Bacillus subtilis CGMCC1.210 has a strong activity of producing α-amylase.

[0035] Trichodermapseudokoningii (Trichodermapseudokoningii) is a strain numbered CMCC3.6612 of the China Industrial Microbiology Culture Collection Management Center; it has a high cellulase activity

[0036] Candida tropicalis is a bacterial strain numbered CMCC2.402 in the China Industrial Microorganism Culture Collection and Management Center; it can assimilate five-carbon sugars.

[0037] 2 specific methods

[0038] (1) Medium: Bacillus subtilis seed medium: LB medium; Candida tropicalis, Trichoderma pseudoconii seed medium: PDA medium;

[0039] (2) Preparation of primary seed liquid: Inoculate Bacillus subtilis in LB medium and incubate at 32°C for 18 hours, inoculate Candida tropicalis and Trichoderma pseudoconii into PDA respectively,...

Embodiment 2

[0057] The processing steps are the same as in Example 1, except that the edible fungi are oyster mushrooms.

Embodiment 3

[0059] The processing steps are the same as in Example 1, except that the edible mushroom is Pleurotus eryngii.

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Abstract

The invention relates to a novel method for extracting fulvic acids from industrially-produced waste edible fungi residues by fermentation. The novel method includes the steps of (1) preparing a primary seed solution; (2) preparing a secondary seed solution; (3) inoculating the secondary seed solution, bacillus subtilis, candida tropicalis and trichoderma pseudokoningii into a 1000-2000L of fermentation tank respectively and fermenting for 10-15 days so as to obtain a fermented seed solution; (4) treating pleurotus ostreatus residues; (5) inoculating the fermented seed solution into the pleurotus ostreatus residues to be inculated, and fermenting at the temperature 20-40 DEG C aerobically for 4-5 days so as to obtain fermented fungi residues; (6) extracting the fulvic acids; (7) performing spray drying so as to obtain flavescent fulvic acid powder. The fulvic acid content can be above 35% after fermentation, and yield of crude fulvic acids can be up to about 30% (dry matter percentage) through extraction.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new method for extracting fulvic acid from waste fungi residues of industrially produced edible fungi. Background technique [0002] Fulvic acid (Fulvic Acids, code name FA) is mainly a complex mixture of macromolecular aromatic-aliphatic hydroxycarboxylic acids containing active functional groups. It has the unique effects of small molecular weight, high active gene, improving soil, increasing ground temperature, promoting root system development, effectively adjusting soil pH, and solving soil compaction. In the case of dry climate, the stomatal opening of crops can be reduced to resist drought and ensure harvest. Its applications are mainly as follows: [0003] Fulvic acid is used in conjunction with other inorganic fertilizers (such as nitrogen, phosphorus, potassium, etc.), which can fix nitrogen, decompose phosphorus, and release potassium slowly, promote the tr...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P1/04C12P1/02C12R1/125C12R1/74C12R1/885
Inventor 李瑾李德舜
Owner SHANDONG UNIV
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