Method for producing fuel ethanol by using chemical method and microorganism to comprehensively treat banana stalk

A fuel ethanol, comprehensive treatment technology, applied in microorganism-based methods, biochemical equipment and methods, biofuels, etc., can solve the problems of long hydrolysis time, high cost, low saccharification rate, etc. significant effect

Inactive Publication Date: 2008-09-10
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overcome the high cost, low saccharification rate and long hydrolysis time of other methods for pretreatment of banana stems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] First, collect the excrement of herbivores, activate it with normal saline for 30-60 minutes, filter the supernatant, add it to the sterilized primary liquid medium containing fiber filter paper, at a temperature of 30°C and a rotation speed of Shake in a shaker at 140r / min for 15 days. Repeat the above steps 3 times to further enrich the strains, and finally filter out the disintegrated filter paper to obtain the original bacterial liquid. Then crush the banana stems, add a certain volume of original microbial bacterial liquid after high-temperature sterilization, shake in a shaker with a pH value of 8.0, a temperature of 30°C, and a rotation speed of 140 r / min for 20 days, remove the dry matter, and separate the obtained Bacteria that have a good degradation effect on banana stems. Then the isolated microbial strains were drawn and purified on the PDA solid plate medium, and the purified strains were stored in a refrigerator below 4°C. Take 2Kg of banana stems crush...

Embodiment 2

[0024] Add the pure strains that have been screened and stored in the refrigerator below 4°C to the pre-high-temperature sterilized culture solution containing nutrients and mineral elements, and adjust the pH of the solution to 8.0 at a temperature of 30°C and a speed of 140r / min Proliferate and cultivate in a shaker for about 24 hours to obtain a microbial bacterial liquid. Banana stem is carried out pretreatment with sodium hydroxide solution, and its step is: get 2Kg fresh banana stem and pulverize to about 3cm long, wide about 0.5cm, add 24Kg mass percent concentration and be 6% sodium hydroxide solution, at 30 ℃ Leave it for 4 hours, and adjust the pH of the pretreatment solution to 7.0. Next, add 0.6Kg microbial bacterial liquid and 0.06Kg yeast. Mix the mixture of bacteria and yeast, the pretreated banana stalks and the pretreatment solution of pH8.0 together, stir evenly, put it into a fermentation container, and seal it for fermentation at 30°C for 20 days. Finally...

Embodiment 3

[0026] Add the pure strains that have been screened and stored in the refrigerator below 4°C to the pre-high-temperature sterilized culture solution containing nutrients and mineral elements, and adjust the pH of the solution to 8.0 at a temperature of 30°C and a speed of 140r / min Proliferate and cultivate in a temperature-controlled shaker for about 24 hours to obtain a microbial bacterial liquid. Banana stem is carried out pretreatment with sodium hydroxide solution, and its step is: get 2Kg fresh banana stem and pulverize to about 3cm long, wide about 0.5cm, add 24Kg mass percent concentration and be 10% sodium hydroxide solution, at 40 ℃ Leave it for 3 hours and adjust the pH to 8.0. Next, add 0.2Kg microbial bacterial liquid and 0.02Kg yeast. Mix the mixture of bacteria and yeast, the pretreated banana stalks and the pretreatment solution of pH8.0 together, stir evenly, put it into a fermentation container, and seal it for fermentation at 30°C for 20 days. Finally, filt...

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Abstract

The invention discloses a method for processing banana stalks with integrated treatment by chemical method and microbiology to prepare fuel ethanol, comprising the following steps of: (1) cutting the banana stalks, adding sodium hydroxide solution with the mass concentration between 1 and 11 percent, hydrolyzing for 1 to 6h under the condition between 25 and 45 DEG C, and regulating the pH value of hydrolysate between 6.0 and 8.0 after hydrolyzing; (2) processing the pretreated banana stalks to be inoculated with microbial solution and added with yeast to lead the mass ratio of the banana stalks to microbial solution to the yeast of 1 to between 0.1 and 0.5 to between 0.01 and 0.05; (3) processing the inoculated banana stalks to be evenly agitated and filled into a fermentation vessel, and processing closed fermentation for 15 to 30 days under the condition between 25 to 30 DEG C; (4) preparing the fuel ethanol by atmospheric distillation, which is to distill the fermented material to acquire the fuel ethanol. The invention processes the banana stalks with the integrated treatment by the chemical method and the microbiology to enable the saccharification technology of the banana stalks to be easily operated so as to improve the saccharification rate, and shortens the period of using the banana stalks to prepare ethanol, thereby reducing the manufacturing cost of ethanol.

Description

technical field [0001] The invention belongs to the technical field of comprehensive utilization of biomass energy and crop fiber, and in particular relates to a method for comprehensively treating banana stems with chemical methods and microorganisms to produce fuel ethanol. Background technique [0002] Biomass is the fourth largest energy source in the world. Since the early 1970s, the development and utilization of biomass energy has become a worldwide hot research topic. Biomass energy is a form of energy in which solar energy is stored in organisms in the form of chemical energy. It is a renewable energy source that is inexhaustible and inexhaustible. It is an important component of the world's energy sustainable development strategy part. Compared with many renewable energy sources, biomass energy has the advantages of large reserves, wide distribution, and obvious environmental protection effects, and has great potential for development. After entering the new cent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 张焜邓强蔡燕飞赵肃清方岩雄黄宝华
Owner GUANGDONG UNIV OF TECH
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