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Preparation process of lactic acid bacteria lysis complex

A preparation process and compound technology, applied in the direction of biochemical equipment and methods, bacteria, microorganisms, etc., can solve the problems of cumbersome production and preparation processes, and achieve the effects of process simplification, efficient preparation, and easy operation

Inactive Publication Date: 2020-06-26
YANGZHOU ZHENWEI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production and preparation process of lactic acid bacteria lysate is cumbersome. In order to obtain high-quality lactic acid bacteria lysate complex, a complete and simplified production process is required.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Lactic acid bacteria lysate complex preparation process of the present invention comprises the following steps:

[0017] 1) Preparation of seed solution: inoculate Lactobacillus plantarum, Bifidobacterium longum, and Lactobacillus paracasei in MRS test tube culture medium, culture at 37°C for 22 hours, and then insert the test tube strains into the liquid MRS medium in the Erlenmeyer flask Fermentation culture, cultured at 37°C for 35h;

[0018] 2) Fermentation: Inoculate each seed solution in a fermenter according to the inoculum amount, and ferment at 37°C for anaerobic fermentation; the inoculation amounts of Lactobacillus plantarum, Bifidobacterium longum, and Lactobacillus paracasei are 3%, 5%, % and 3%;

[0019] 3) Homogeneous wall breaking: pass the fermented lactic acid bacteria fermentation liquid through a high-pressure homogenizer to homogeneously break the wall to obtain the wall breaking liquid; the conditions for the homogeneous wall breaking are: the pre...

Embodiment 2

[0023] Lactic acid bacteria lysate complex preparation process of the present invention comprises the following steps:

[0024] 1) Preparation of seed liquid: inoculate Lactobacillus plantarum, Bifidobacterium longum, and Lactobacillus paracasei in MRS test tube culture medium respectively, culture at 38°C for 26 hours, and then insert the test tube strains into the liquid MRS medium in the Erlenmeyer flask Fermentation culture, cultured at 38°C for 38h;

[0025] 2) Fermentation: Inoculate each seed liquid into a fermenter according to the inoculum amount, and ferment at 38°C for anaerobic fermentation; the inoculum amounts of Lactobacillus plantarum, Bifidobacterium longum, and Lactobacillus paracasei are 4%, 6%, % and 4%;

[0026] 3) Homogeneous wall breaking: pass the fermented lactic acid bacteria fermentation liquid through a high-pressure homogenizer, and homogeneously break the wall to obtain a wall breaking liquid; the conditions for homogeneous wall breaking are: the...

Embodiment 3

[0030] Lactic acid bacteria lysate complex preparation process of the present invention comprises the following steps:

[0031] 1) Preparation of seed liquid: Inoculate Lactobacillus plantarum, Bifidobacterium longum, and Lactobacillus paracasei in MRS test tube culture medium respectively, culture at 37°C for 24 hours, and then insert the test tube strains into the liquid MRS medium in the Erlenmeyer flask Fermentation culture, cultured at 37°C for 36h;

[0032] 2) Fermentation: Inoculate each seed solution in a fermenter according to the inoculum amount, and ferment at 37°C for anaerobic fermentation; the inoculation amounts of Lactobacillus plantarum, Bifidobacterium longum, and Lactobacillus paracasei are 3%, 5%, % and 3%;

[0033] 3) Homogeneous wall breaking: pass the fermented lactic acid bacteria fermentation liquid through a high-pressure homogenizer to homogeneously break the wall to obtain the wall breaking liquid; the conditions for the homogeneous wall breaking a...

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Abstract

The invention provides a preparation process of lactic acid bacteria lysis complex, relates to an extraction technology of a microbial complex, and in particular relates to a preparation process of the lactic acid bacteria lysis complex. The complete simplified process for the extraction of the lactic acid bacteria lysis complex is provided. The preparation process comprises the following steps: preparing a seed solution: inoculating lactobacillus plantarum, bifidobacterium longum and lactobacillus paracasei in MRS test tube culture media respectively, performing culture at 37-38 DEG C for 22-26 h, inoculating the test tube strain to a triangular flask liquid MRS culture medium for fermentation culture, and performing culture at 37-38 DEG C for 35-38 h; performing fermentation: inoculatingthe seed liquids into a fermentor according to the inoculation amounts, and performing anaerobic fermentation; performing homogenization wall breaking: performing treatment on the fermented lactic acid bacteria fermentation liquid in a high-pressure homogenizer, and performing homogenization wall breaking to obtain a broken-wall liquid; performing centrifugation: performing centrifugation on thehomogenized broken-wall liquid in a tubular centrifuge to obtain a broken-wall supernatant after centrifugation; and performing filtration: performing filtration on the centrifuged broken-wall supernatant in a food-grade liquid filter to obtain the lactic acid bacteria lysis complex. The method provided by the invention is simple, the process is simplified, and the operation is easy.

Description

technical field [0001] The invention relates to a microbial compound extraction technology, in particular to a preparation process of a lactic acid bacteria lysed compound. Background technique [0002] Lactic acid bacteria or their fermented biological products have beneficial physiological effects on the host by regulating the function of the host immune system and improving the balance of the host flora, and are considered to have the function of improving the micro-ecological environment of the human intestinal tract. Its functions are: (1) improving the intestinal micro-ecological environment; (2) improving immunity; (3) relaxing the bowel and improving intestinal function; (4) inhibiting the production of endotoxin and delaying aging. As a new type of biological resource, the intracellular product of lactic acid bacteria has brought more positive significance to daily chemical and food through the research and development of its industrialization. However, the product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/06C12R1/25C12R1/01C12R1/225
CPCC12N1/066C12N1/20
Inventor 朱臻
Owner YANGZHOU ZHENWEI BIOTECH CO LTD
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