Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for catalytically synthesizing sorbate with enzyme method

A technology of sorbate and sorbic acid, which is applied in the field of lipase-catalyzed synthesis of sorbate, can solve the problem that the yield is less than 80%, and achieve the effects of simple separation, low environmental pollution and high reaction conversion rate

Active Publication Date: 2016-11-09
浙江英沃迪生物科技有限公司
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is to catalyze the synthesis of ethyl sorbate in the water phase, and the maximum yield is less than 80%.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for catalytically synthesizing sorbate with enzyme method
  • Method for catalytically synthesizing sorbate with enzyme method
  • Method for catalytically synthesizing sorbate with enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In a sealed 10ml Erlenmeyer flask, dissolve 0.04g sorbic acid and 0.4ml absolute ethanol in 3.6ml tert-butanol, add 0.2g lipase Novozyme 435 (enzyme activity 10000U / g) and 0.4g 4A molecular sieve (purchased from Aladdin Reagent (Shanghai) Co., Ltd.), oscillating and reacting at a constant temperature of 30° C. for 10 hours at an oscillating speed of 200 r / min, sampling for gas chromatography analysis, and the conversion rate of sorbic acid was 81.6%.

[0023] Gas chromatograph measurement conditions for sorbic acid Ⅰ or product Ⅱ: HP-5 capillary column; constant temperature of column oven at 150°C; carrier gas: nitrogen; column flow rate: 1ml / min; FID detector.

Embodiment 2

[0025] In a sealed 10ml Erlenmeyer flask, dissolve 0.04g sorbic acid and 0.4ml absolute ethanol in 3.6ml tert-butanol, add 0.2g lipase Novozyme 435 and 0.4g 4A molecular sieve, and shake the reaction at 40°C for 10 hours at a constant temperature. It was 200r / min, sampling was carried out by gas chromatography, and the conversion rate of sorbic acid was 90.5%.

Embodiment 3

[0027] In a sealed 10ml Erlenmeyer flask, dissolve 0.04g sorbic acid and 0.4ml absolute ethanol in 3.6ml tert-butanol, add 0.2g lipase Novozyme 435 and 0.4g 4A molecular sieve, and shake at 50°C for 10 hours at a constant temperature. It was 200r / min, sampling was carried out by gas chromatography, and the conversion rate of sorbic acid was 96.2%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for catalytically synthesizing sorbate with an enzyme method. The method takes sorbic acid and C1 to C4 alcohols as reaction substrates, takes lipase as a catalyst and takes an organic solvent as a reaction medium; a water absorbent is added and an esterification reaction is carried out under conditions of 30 DEG C to 60 DEG C and 200rpm; after the reaction is completed, a reaction solution is separated and purified to obtain the sorbate. The preparation method of the sorbate, disclosed by the invention, has the main beneficial effects that the regioselectivity of enzymes is high and the reaction conversion rate is high; downstream separation is simple, energy consumption is low and environment pollution is small; the preparation method is suitable for industrial production and the esterification rate is 90 percent to 99 percent.

Description

(1) Technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for synthesizing sorbic acid ester catalyzed by lipase. (2) Background technology [0002] Sorbic acid is a kind of unsaturated fatty acid, which has excellent activity of inhibiting yeast, mold and aerobic bacteria, and can inhibit the growth and reproduction of microorganisms, so as to effectively prolong the preservation time of food. However, since sorbic acid is a naturally occurring organic acid, it is easily decomposed and metabolized by microorganisms, and its antibacterial effect is short, and its antibacterial effect is not strong enough. It has limited ability to inhibit microorganisms, and is ineffective against lactic acid bacteria and anaerobic spores. It is used under certain conditions, and the scope of use is greatly limited. In order to improve the antibacterial range of sorbic acid, it is necessary to carry out esterificat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62
CPCC12P7/62
Inventor 郑建永汪钊章银军黄丽娟
Owner 浙江英沃迪生物科技有限公司