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Extraction method of lentinan

A technology of lentinan and extraction method, which is applied in the direction of anti-toxic agent, drug combination, etc., can solve the problems of hindering the development of lentinan industry, low extraction rate of lentinan, and low activity, so as to improve the total antioxidant capacity, increase the extraction rate, Improves the effect of antioxidant and anti-aging benefits

Active Publication Date: 2016-11-16
山东大树达孚特膳食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the extraction rate of lentinan is low and the activity is not high, which greatly hinders the development of lentinan industry

Method used

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  • Extraction method of lentinan

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Take shiitake mushrooms, remove impurities and wash, drain and chop;

[0024] 2) Put the shiitake mushrooms obtained in step 1) into a high-pressure tank, add deionized water 4 times the weight of shiitake mushrooms, pressurize to 18MPa, perform high-pressure treatment for 3 minutes, and release the pressure to obtain a paste-like slurry;

[0025] 3) Add deionized water 12 times the weight of the original shiitake mushrooms to the paste slurry, then add a compound enzyme of 0.03% of the weight of the original shiitake mushrooms, adjust the pH to 5.0, and conduct ultrasonic extraction at 39° C. for 5 minutes at a frequency of 110 kHz, and then Continue to raise the temperature to 50°C for ultrasonic extraction for 10 minutes to obtain the extract, the compound enzyme is a mixture of pectin lyase, cellulase, pepsin and lipase;

[0026] 4) Suction filter the extract, compress the filtrate to 1 / 4 of the volume of the original filtrate, then add an ethanol solution with a...

Embodiment 2

[0028] 1) Take shiitake mushrooms, remove impurities and wash, drain and chop;

[0029] 2) Put the shiitake mushrooms obtained in step 1) into a high-pressure tank, add deionized water 5 times the weight of shiitake mushrooms, pressurize to 16MPa, perform high-pressure treatment for 5 minutes, and release the pressure to obtain a paste-like slurry;

[0030] 3) Add deionized water 10 times the weight of the original shiitake mushrooms to the paste slurry, then add a compound enzyme of 0.05% of the weight of the original shiitake mushrooms, adjust the pH to 4.8, and conduct ultrasonic extraction at 39° C. for 6 minutes at a frequency of 130 kHz, and then Continue to raise the temperature to 49°C for ultrasonic extraction for 9 minutes to obtain the extract, and the compound enzyme is a mixture of pectin lyase, cellulase, pepsin and lipase;

[0031] 4) Suction filter the extract, compress the filtrate to 1 / 3 of the volume of the original filtrate, then add 13 times the weight of ...

Embodiment 3

[0033] 1) Take shiitake mushrooms, remove impurities and wash, drain and chop;

[0034] 2) Put the shiitake mushrooms obtained in step 1) into a high-pressure tank, add deionized water 6 times the weight of shiitake mushrooms, pressurize to 14MPa, perform high-pressure treatment for 7 minutes, and release the pressure to obtain a paste-like slurry;

[0035] 3) Add deionized water 9 times the weight of the original shiitake mushrooms to the paste slurry, then add a compound enzyme of 0.07% of the weight of the original shiitake mushrooms, adjust the pH to 4.5, and conduct ultrasonic extraction at 38° C. for 8 minutes at a frequency of 150 kHz, and then Continue to raise the temperature to 48°C for ultrasonic extraction for 8 minutes to obtain the extract, and the compound enzyme is a mixture of pectin lyase, cellulase, pepsin and lipase;

[0036] 4) Suction filter the extract, compress the filtrate to 1 / 4 of the volume of the original filtrate, add 15 times the weight of the or...

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Abstract

The invention belongs to the technical field of biological extraction, and particular relates to an extraction method of lentinan. The extraction method comprises the following steps of (1) taking shiitake mushrooms, removing impurities, thoroughly cleaning the shiitake mushrooms without the impurities, draining the cleaned shiitake mushrooms, and cutting up the drained shiitake mushrooms; (2) performing high-pressure treatment so as to obtain paste slurry; (3) adding water and compound enzymes to the paste slurry, and performing ultrasonic extraction under certain conditions according to temperature segments; and (4) performing suction filtration, compressing filtrate, performing alcohol deposition, performing alcohol precipitation, performing centrifugation, abandoning supernatant, and performing vacuum freeze drying so as to obtain the lentinan. The lentinan prepared by the extraction method disclosed by the invention is high in extraction rate and purity, and high in oxidation resistance and ageing resistance.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, in particular to a method for extracting lentinan. Background technique [0002] my country has become a major producer of edible fungi. By 2013, the production of edible fungi produced in my country accounted for more than 70% of the world's total output. The production of edible fungi has become the three major rural industries with equal emphasis on planting and breeding. It is an important way for farmers in mountainous or poor areas of our country to get rid of poverty and become rich. However, there are serious phenomena such as large consumption of raw materials, low economic efficiency, and inverted output ratio in the production of edible fungi in my country. Research on a medium formula to improve the yield and quality of edible fungi has become a new demand in the edible fungus market. [0003] Shiitake mushrooms belong to the phylum Fungi, Agaricaceae, Pleurotaceae, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06
CPCC08B37/0003C08B37/0024
Inventor 高枫
Owner 山东大树达孚特膳食品有限公司
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