Traditional Chinese medicine composition for radiotherapy and chemotherapy vomiting reaction reliving and synergetic cancer inhibition
A combination, radiotherapy and chemotherapy technology, applied in the directions of drug combination, digestive system, anti-tumor drugs, etc., can solve the problems of large prescription and high dosage, and achieve the effect of maintaining appetite, enhancing tumor suppressor effect, and reducing vomiting reaction.
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Embodiment 1
[0012] Embodiment 1: preparation of dry extract of composition of the present invention
[0013] Take 160g of ginger, 80g of astragalus, 120g of magnolia bark, 100g of salvia miltiorrhiza, 40g of centella asiatica, 100g of atractylodes rhizome, and 120g of hawthorn, chop and mix them separately, and then add water to decoct. After boiling for half an hour, collect the decoction. The residue is decocted with water, so decocted three times, and the decoction liquid is combined. Concentrate the decoction to make a dry extract.
Embodiment 2
[0014] Embodiment 2: Research on the antiemetic effect of the composition of the present invention
[0015] Effect of the composition of the present invention on pica behavior in rats.
[0016] 1. Experimental materials
[0017] 1.1 Samples: 1. The dry extract of the pharmaceutical composition prepared in Example 1 is prepared into a solution with a certain concentration just before use. Preparation method: Take an appropriate amount of the pharmaceutical composition, weigh it accurately, add a certain amount of distilled water, ultrasonically for 10 minutes, and stir until fully dissolved and suspended; ②Ondansetron injection (specification: 4mL·8mg -1 ; Shandong Qilu Pharmaceutical Co., Ltd.; batch number: 9120815AT). 1.2 Reagents: ① cisplatin (powder injection; specification: 10 mg; batch number: 9131256HD; Shandong Qilu Pharmaceutical Co., Ltd.); ② kaolin (analytical pure, prepared by referring to the method of Takeda N et al., mixing kaolin with 1% gum arabic to prepare...
Embodiment 3
[0026] Embodiment 3: Research on the antitumor effect of the composition of the present invention
[0027] 1. Materials and Reagents
[0028] 1.1 Sample:: 1. The dry extract of the pharmaceutical composition prepared in Example 1 is prepared into a solution with a certain concentration just before use. Preparation method:
[0029] Take an appropriate amount of the pharmaceutical composition, weigh it accurately, add a certain amount of distilled water, ultrasonicate for 10 minutes, and stir until the suspension is fully dissolved; ②Cisplatin (powder injection; specification: 10mg; batch number: 9131256HD; Shandong Qilu Pharmaceutical Co., Ltd.) .
[0030] 1.2 Reagents: RPMI-1640, DMEM (Gibco), calf serum (Vicent, 20130118), Trypsin (Amresco).
[0031] 1.3 Cell line: H22 mouse liver cancer cell line, provided by Shanghai Cell Institute of Chinese Academy of Sciences.
[0032] 1.4 Animals: C57BL / 6 mice, 18-22g, half male and half male, provided by the Comparative Medicine Ce...
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