Photosynthetic bacteria microbial ecosystem for aquiculture and application thereof

A technology for photosynthetic bacteria and micro-ecological preparations, applied in bacteria, microorganisms, microorganism-based methods, etc., can solve the problems of small number of viable bacteria, poor bacterial stability, and unsatisfactory quality, and achieve good degradation effect and obvious degradation. effect of effect

Active Publication Date: 2016-11-23
九江大北农水产科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the market is full of various photosynthetic bacterial preparations, most of which exist in liquid and powder form. According to the analysis of various products on the market in the laboratory, their quality is not satisfactory, mainly for the liquid bacterial preparations. The color is not bright, The stability of the bacteria is poor, the number of viable bacteria is small, and some photosynthetic bacteria have not even been found. The urgent demand of the market and the chaotic state of uneven quality of photosynthetic bacteria products on the market provide us with a great opportunity. Therefore, the development of It is of great significance to produce excellent photosynthetic bacteria preparations

Method used

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  • Photosynthetic bacteria microbial ecosystem for aquiculture and application thereof
  • Photosynthetic bacteria microbial ecosystem for aquiculture and application thereof
  • Photosynthetic bacteria microbial ecosystem for aquiculture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 Preparation of Rhodopseudomonas palustris bacteria liquid

[0027] 1. Formula of Rhodopseudomonas palustris expansion medium ( / L):

[0028] CaCl 2 0.1g, KH 2 PO 4 0.5g, NH 4 Cl 1g, MgCl 2 ·6H 2 O 0.5g, NaCl 1g, NaAc·3H 2 O 1g, sodium succinate dihydrate 1g, yeast powder 0.3g, peptone 0.3g

[0029] 2. Expand the culture medium step

[0030] (1) Weigh each component according to the medium formula, dissolve it in water, divide the medium into 5L alcohol-sterilized PET plastic buckets, 4.5L per bucket, inoculate Rhodopseudomonas palustris CGMCC No.9136500mL, Cover. Place it in a cardboard box and cultivate it under incandescent light for 4 days, which can be used as the seed solution for the next step of large-scale cultivation.

[0031] (3) Weigh each component required for 1 ton of culture solution, dissolve it completely with a small amount of tap water first, then fully mix it with the remaining tap water in a large bucket, inoculate 10 buckets o...

Embodiment 2

[0032] Example 2 Degradation ability test of Rhodopseudomonas palustris CGMCC No.9136 to nitrite

[0033] 1. Experimental method

[0034] (1) Make a nitrite standard curve: prepare different concentrations of nitrite solutions and place them in a microplate reader to measure OD 550 , made into salt concentration and OD 550 The corresponding standard curve.

[0035] (2) Culture medium for nitrous reduction experiment: prepare 3° salt water and distribute it in 150mL small triangular flasks, 50mL / bottle, add 0.05g of pond bottom mud to each bottle. 121°C, high-pressure steam sterilization for 20 minutes.

[0036] (3) Set dark conditions and light conditions, set up an experimental group and a control group under each condition, and each strain of bacteria has 3 replicates. The experimental group is added with 50 μL of photosynthetic bacteria seed solution, placed in dark and light conditions respectively, and cultured at 30°C .

[0037] (4) Measure the nitrite content every...

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Abstract

The invention relates to the technical field of microecologics, in particular to rhodopseudomonas palustris for improving the aquiculture environment. The preservation number of the rhodopseudomonas palustris is CGMCC No.9136. The microecologics adopting the CGMCC No.9136 has a good effect on degrading nitrite in the culture environment, and the nitrate content can be degraded by 98% within 24 h.

Description

technical field [0001] The invention belongs to the technical field of microecological application, and in particular relates to a microecological preparation of Rhodopseudomonas palustris and its application in aquaculture. Background technique [0002] In the aquaculture industry, the cleanliness, richness and beauty of the water body are important indicators to measure the quality of the aquaculture environment. Clear means to keep the water body clear; rich means that the water body is rich in algae and zooplankton to provide sufficient bait for aquatic animals; beauty means that the water body environment and aquatic animals form an ecological balance. At present, due to the high-density farming in ponds, the excrement and residual bait of farmed animals continue to accumulate, harmful microorganisms and algae multiply, and the water body is severely hypoxic, which leads to the serious phenomenon of fish and shrimp floating heads and stealing dead. The use will cause t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
Inventor 汪攀王蕊李亚刘婷何增国朱传忠郑慧
Owner 九江大北农水产科技有限公司
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