Composition for preventing adhesion, containing surface-modified chondrocyte-derived extracellular matrix membrane as active ingredient

A technology for preventing adhesion and cartilage cells, applied in medical preparations containing active ingredients, organic active ingredients, drug combinations, etc., can solve the problems of hindering wound healing, difficult to maintain the effect of anti-adhesion agents, and causing inflammatory reactions, etc. Excellent anti-blocking effect, excellent anti-blocking effect, increased anti-blocking effect

Active Publication Date: 2016-11-23
AJOU UNIV IND ACADEMIC COOP FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also, this anti-adhesion agent can be recognized as a foreign substance and trigger an inflammatory response or otherwise prevent wound healing
Also, although adhesions may occur for a long time after surgery, the currently used anti-adhesion agents are difficult to maintain their effect because they are broken down quickly

Method used

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  • Composition for preventing adhesion, containing surface-modified chondrocyte-derived extracellular matrix membrane as active ingredient
  • Composition for preventing adhesion, containing surface-modified chondrocyte-derived extracellular matrix membrane as active ingredient
  • Composition for preventing adhesion, containing surface-modified chondrocyte-derived extracellular matrix membrane as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] making chondrocyte-derived extracellular matrix membrane

[0027] Cells from sterile porcine knee cartilage tissue were obtained and transplanted at a high concentration to obtain a membrane morphology. The membrane obtained by the above method is decellularized and washed with an enzyme such as deoxyribonuclease I (DNase I) to obtain a chondrocyte-derived extracellular matrix membrane. The main components are collagen and proteoglycans (refer to Korean Patent Publication No. 10-0816395) ( figure 1 ).

Embodiment 2

[0028] Surface modification of chondrocyte-derived extracellular matrix membrane

[0029] The film obtained in Example 1 was put into ethyl (dimethylaminopropyl) carbodiimide (ethyl (dimethylaminopropyl) carbodiimide; EDC) / N-hydroxysuccinimide (N-Hydroxysuccinimide; NHS) Solutions were prepared at 50 mM in distilled water and processed with shaking at 250 rpm for 12 hours and at room temperature. Then, poly-L-lysine (poly-L-lysine; PLL) was prepared in distilled water at 10 ug / ml, put into the EDC / NHS treated membrane and shaken at 250 rpm for 24 hours and treated at room temperature. After the treatment, the films were washed three times in triple distilled water and dried for 10 minutes each time. In order to measure the surface potential difference of the surface-modified film, it is fixed on a glass plate, and monitoring particles that can move due to electricity are placed in 10mM sodium chloride, and the fixed film is covered and the current flows. From this, the ch...

Embodiment 3

[0031] Fibroblast attachment experiment

[0032] In this example, using the cartilage-derived extracellular matrix membranes prepared in the cell culture dish, Example 1 and Example 2, the difference in attachment rate of fibroblasts that play a role in the adhesion mechanism was confirmed by the following method.

[0033] The cartilage-derived extracellular matrix membrane prepared in Example 1 and the modified membrane prepared in Example 2 were attached to different 24-well dishes (well dish) with a diameter of 5 mm, dried and fixed, and carried out Ethylene oxide gas (EOgas) sterilization. And transplant 2×10 in the dishes coated with each membrane and in the 24-well plates (well plate) not coated. 4Fibroblasts were allowed to attach for 24 hours. Then, non-attached cells were washed with PBS buffer, and MTT assay (MTT assay) was performed to confirm cells attached to each surface (measured at absorbance at 460 nm).

[0034] Such as image 3 As shown, as a result, t...

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PUM

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Abstract

The present invention relates to a composition for preventing adhesion, containing a surface-modified chondrocyte-derived extracellular matrix membrane as an active ingredient, and it was observed that the efficiency of adhesion of fibrocytes to a chondrocyte-derived extracellular matrix material in vitro is low and an excellent adhesion prevention effect is exhibited in a subcutaneous adhesion animal model using a mouse and in an intestinal adhesion model using a rat. In addition, it was ascertained that the adhesion prevention effect remarkably increases when the surface charge of the present material is modified to be neutral by using poly-L-lysine (PLL), and thus the chondrocyte-derived extracellular matrix material can be applied as an adhesion barrier with excellent in vivo stability and an adhesion prevention effect.

Description

technical field [0001] The present invention relates to the use of a composition containing a surface-modified chondrocyte-derived extracellular matrix membrane as an active ingredient for preventing adhesion. Background technique [0002] Adhesion refers to the phenomenon that the tissue surfaces of organs that need to be separated from each other are connected and fused into fibrous tissue due to surgery or inflammation, and complications such as small bowel obstruction, acquired female infertility, ectopic pregnancy, chronic abdominal pain, and reoperation occur due to this . Such adhesions occur in 50-90% of surgical procedures, and as many as 34.6% of patients undergo reoperation because of adhesions. Adhesions are similar to the healing process exhibited after trauma, so methods to prevent adhesions without interfering with the healing process are particularly important. Adhesion is the phenomenon that during the healing process of the surgical site, the normally dep...

Claims

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Application Information

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IPC IPC(8): A61K35/32A61K35/12A61K31/198A61K9/70A61P43/00
Inventor 闵炳显朴索拉崔秉显宋宝拉
Owner AJOU UNIV IND ACADEMIC COOP FOUND
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