Poultry feed additive
A feed additive, poultry technology, applied in the direction of animal feed, animal feed, animal husbandry, etc., can solve the problem of undetermined Pediococcus pentosaceus, etc., and achieve the effects of improving meat production performance, increasing action time, and enhancing immunity
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Embodiment 1
[0029] Mix the cultured Pediococcus pentosaceae and Leuconostococcus mesentericus bacteria liquid according to the ratio of 0.4:1, then centrifuge and concentrate, add biological selenium, sodium alginate, calcium chloride, skimmed milk powder and lactose in proportion, after coating and curing , collected microcapsule particles by centrifugation at 350r / min at low speed for 10min, and after washing 3 times, a small sample was taken out to measure the embedding yield; the remaining products were pre-cooled at -20°C for 10min, frozen at -80°C overnight, and then freeze-dried for 24h. The product is packed in a sealed bag and stored at room temperature. The specific ratio of biological selenium, sodium alginate, calcium chloride, skimmed milk powder and lactose is shown in Table 1. The probiotic microcapsules are uniform and round particles, and the diameter of the probiotic microcapsules is 0.6-1.5 mm.
[0030] Weigh a certain amount of polylactic acid particles into the organi...
Embodiment 2
[0035] Biological selenium is prepared by comprising the following steps,
[0036] Step 1, configure the medium, including glucose 5.0g, casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, potato 50g, ascorbic acid 0.5g, sodium acetate 5.0g, Tween 80 1.0g, dihydrogen phosphate Potassium 2.0g, triammonium citrate 3.7g, magnesium sulfate heptahydrate 0.2g, manganese sulfate 0.05g, calcium carbonate 20.0g, agar 20g, pH natural, dilute to 1L, sterilize at 115°C for 30min;
[0037] Step 2: Add sodium selenite to the medium, the selenium concentration is 80 μg / mL, inoculate Saccharomyces cerevisiae and Candida in the medium at a ratio of 1:1, and inoculate in a rotary shaker at 30°C and 140r / min , shaking culture for 12h;
[0038]Step 3: Put the fermentation broth into a dialysis bag after ultrasonic crushing, tie the upper end tightly, dialyze in distilled water, add 1mL of 150g / L cysteine solution in distilled water, and continuously drop methylene blue solution until...
Embodiment 3
[0040] Oligosaccharide-trans-2-hexenal is prepared by the following method: weigh oligosaccharide and trans-2-hexenal in proportion, dissolve oligosaccharide and trans-2-hexenal respectively in acetone, Add the oligosaccharide-acetone solution into a three-necked flask with a thermometer, add an appropriate amount of DMAP, and slowly add trans-2-hexenal dropwise under the condition of heating and refluxing, and after heating and refluxing for 20 hours, cool naturally, and add Excessive distilled water, after the product is fully precipitated, the precipitate is collected by filtration and then recrystallized with ethanol, the yield is 82.1%.
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