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Method for identifying peanut black rot resistant variety at seedling stage

A technology of black rot and black rot bacteria, applied in the agricultural field, can solve the problems of weak basic disease research, unsystematic research of resistance evaluation of peanut varieties and germplasm resources, etc., and achieve high repeatability, simple method and good stability Effect

Inactive Publication Date: 2016-12-07
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since this disease was only discovered in my country in recent years, the basic research on the disease is relatively weak, and there is no systematic research on the resistance evaluation of existing peanut varieties and germplasm resources.

Method used

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  • Method for identifying peanut black rot resistant variety at seedling stage
  • Method for identifying peanut black rot resistant variety at seedling stage
  • Method for identifying peanut black rot resistant variety at seedling stage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The experiment of inoculation conditions was carried out with Fuyu four-grained red peanut variety (purchased from Xingkubao Peanut Purchasing Department, Sanjingzi Town, Fuyu County, Jilin Province) as the material. details as follows:

[0024] 1. Preparation of mycelia suspension

[0025] The peanut black rot bacteria stored at 4°C were inoculated on a PDA medium plate, and cultured in a constant temperature incubator at 25°C for 5 days. Cut 10 pieces of mycelium with a hole punch with a diameter of 1 cm, put them into a 300 mL Erlenmeyer flask containing 150 mL of PD culture solution, and place them in a constant temperature shaker at 25 °C and 180 r / min for 5 d. Filter the bacterial solution with sterilized four-layer gauze, pick out the residual agar block, scrape the mycelia on the gauze, put the mycelium on sterile filter paper, squeeze and absorb the water, weigh 5.0g of mycelium and add it to 95mL 4 ℃ sterile water, homogenate for 2min. Microscopically inspe...

Embodiment 2

[0060] With 128 peanut varieties (provided by Professor Zheng Yixiong of Zhongkai Agricultural Engineering College, see Table 6) as materials, the concentration of inoculated mycelia suspension was 0.5% (mass / volume), soaking time was 10min, and sterile water was used as a control. Evaluate varieties for disease resistance. Each variety was replicated 3 times, with 10 plants per replicate. After soaking and inoculating, put the peanut sprouts into a tray lined with double-layer wet gauze, cover with double-layer wet gauze, keep moist at a constant temperature of 25°C for 2 days, then insert the peanut sprouts into the hydroponic cotton, and place them in the light for cultivation at 25°C Cultivated in the room. Pull out the plants after 10 days, observe the roots according to the grading standards, record the root rot grade and the number of infected plants, calculate the disease index, and evaluate the disease resistance of the tested varieties or materials.

[0061] The st...

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Abstract

The invention belongs to the technical field of agriculture and particularly discloses a method for identifying a peanut black rot resistant variety at a seedling stage. 0.5-5% of peanut black rot hyphe suspension is adopted to soak peanut buds; after the peanut buds are soaked for a period of time, moisture retention is performed for 2 d and then water culture is performed; that is, an indoor inoculation and identification method is adopted, and the peanut variety resistance can be identified at the seedling stage. The method is simple, convenient and efficient, obtained results are good in stability, the repeatability is high, and the theoretical basis is provided for peanut black rot resistance breeding and field application of the field.

Description

technical field [0001] The invention relates to the technical field of agriculture, and more specifically, relates to a method for identifying peanut black rot resistant varieties at the seedling stage. Background technique [0002] Peanut black rot (Cylindrocladium black rot of peanut, referred to as CBR) is an important disease that seriously affects peanut production. Peanut black rot fungus infects the underground tissues of peanuts such as needles, stem bases, roots and fruit pods. The infected parts turn black and rot. die. So far, countries that have reported occurrence of peanut black rot include the United States, Brazil, Japan, South Korea, North Korea, India, Iran, Cameroon, and Australia. In 2009, peanut black rot was first discovered in Guangdong, my country, and then Fujian and Jiangxi reported the occurrence of the disease. [0003] The breeding of disease-resistant varieties is one of the key points in the research on the prevention and control of peanut b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01C1/00
CPCA01G31/00A01C1/00
Inventor 向梅梅袁汇涛张云霞董章勇施祖荣
Owner ZHONGKAI UNIV OF AGRI & ENG