Effective part of Corydalis conspersa as well as preparation method and application of effective part

An effective part, the technology of Corydalis variegata, applied in the field of plant extracts, can solve problems that have not been seen yet

Inactive Publication Date: 2016-12-07
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the research and application of Viola variegata on inhibiting CDC25 and HDAC1 enzymes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiments 1 to 3 are specific implementations of the active part of Viola variegata and its preparation method of the present invention, wherein embodiment 1 is the best embodiment.

[0023] Example 1

[0024] The preparation method comprises the following steps: pulverize the whole plant of Corydalis variegata, ultrasonically extract it with an ethanol solution with a concentration of 75% to 85% by volume for 1 hour, then filter, reflux the filter residue for 3 times, each time for 0.5 hour, combine the extracts, concentrate Dry to obtain the medicinal extract; disperse the medicinal extract with distilled water to obtain a medicinal extract dispersion, then sequentially use petroleum ether, ethyl acetate and n-butanol as solvents to extract the medicinal extract dispersion, and take the ethyl acetate extract and the n-butanol extract respectively The solvent is volatilized to obtain the ethyl acetate fraction and the n-butanol fraction of Corydalis variegata; after ...

Embodiment 2

[0026] The preparation method comprises the following steps: pulverize the whole plant of Corydalis variegata, ultrasonically extract it with an ethanol solution with a concentration of 85% to 95% by volume for 3 hours, then filter, and extract the filter residue by reflux for 3 times, each time for 1 hour, combine the extracts, concentrate Dry to obtain the medicinal extract; disperse the medicinal extract with distilled water to obtain a medicinal extract dispersion, then sequentially use petroleum ether, ethyl acetate and n-butanol as solvents to extract the medicinal extract dispersion, and take the ethyl acetate extract and the n-butanol extract respectively The solvent is volatilized to obtain the ethyl acetate fraction and the n-butanol fraction of Corydalis variegata; after the extract dispersion is extracted, the remaining liquid is concentrated and dried to obtain the aqueous phase extraction fraction of Corydalis variegata.

Embodiment 3

[0028] The preparation method comprises the following steps: pulverize the whole plant of Corydalis variegata, ultrasonically extract it with an ethanol solution with a concentration of 65% to 75% by volume for 0.5 hours, then filter, and extract the filter residue twice by refluxing for 2 hours each time, combine the extracts, concentrate Dry to obtain the medicinal extract; disperse the medicinal extract with distilled water to obtain a medicinal extract dispersion, then sequentially use petroleum ether, ethyl acetate and n-butanol as solvents to extract the medicinal extract dispersion, and take the ethyl acetate extract and the n-butanol extract respectively The solvent is volatilized to obtain the ethyl acetate fraction and the n-butanol fraction of Corydalis variegata; after the extract dispersion is extracted, the remaining liquid is concentrated and dried to obtain the aqueous phase extraction fraction of Corydalis variegata.

[0029] The petroleum ether extracts obtain...

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PUM

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Abstract

The invention provides an effective part of Corydalis conspersa as well as a preparation method and an application of the effective part and belongs to the technical field of plant extracts. The effective part of the Corydalis conspersa is selected from one of an ethyl acetate extract of the Corydalis conspersa, an n-butyl alcohol extract of the Corydalis conspersa and an aqueous extraction extract of the Corydalis conspersa or a mixture of two or more of the extracts of the Corydalis conspersa in any mass ratio. The preparation method comprises steps as follows: the herb of the Corydalis conspersa is ground, an ethanol solution with volume percentage concentration being 65%-95% is added for reflux extraction, an obtained extracting solution is concentrated and dried, and extractum is obtained; distilled water is added to the extractum, the extractum is dispersed, an extractum dispersion liquid is obtained and is extracted with petroleum ether, ethyl acetate and n-butyl alcohol as solvents sequentially, and after solvents are volatilized, the ethyl acetate extract of the Corydalis conspersa and the n-butyl alcohol extract of the Corydalis conspersa are obtained; the extractum dispersion liquid after extraction is concentrated and dried, and the aqueous extraction extract of the Corydalis conspersa is obtained. According to the application of the effective part of the Corydalis conspersa in preparation of drugs for inhibiting CDC25 enzyme and HDAC1 enzyme, the effective part of the Corydalis conspersa can be used for preparing antitumor drugs.

Description

technical field [0001] The active part of Viola variegata and its preparation method and application belong to the technical field of plant extracts. Background technique [0002] Corydalis conspersa Maxim. is a plant of the genus Corydalis Papaveraceae [see: Flora of China. Volume 32. Beijing: Science Press, 1999: 266], which is a traditional medicinal plant in Tibetan medicine. Distributed in southwestern Gansu, central and southern Qinghai, northwest and western Sichuan, and eastern and central Tibet in China. It grows on rocky river banks and alpine gravel at an altitude of (3800-5700) meters. "Chinese Tibetan Medicine" records that its whole plant is used for plague, fire and burns, and fever of Chiba disease. Indications for febrile infectious diseases, gastritis, gastric ulcer, cold and fever, external treatment of sores. [0003] Malignant tumor is one of the major diseases that seriously endanger human health and has become the leading cause of human death. The p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66A61P35/00
CPCA61K36/66A61K2236/333A61K2236/39A61K2236/55
Inventor 林鹏程吴疆吴江却生包婷雯
Owner QINGHAI UNIV FOR NATITIES
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