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Immune enhancer for pig vaccine, and preparation method and application thereof

An immune enhancer and vaccine technology, applied in the field of veterinary biological products, can solve the problems of no effective vaccine prevention and control of infectious diseases, reduced vaccine protection, low vaccine immune efficacy, etc., to improve the level of cellular immune response, easy to store, good safety effect

Inactive Publication Date: 2016-12-07
WUHU YINGTE FEIER BIOLOGICAL PROD IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional vaccines and new vaccines have also encountered some intractable problems: vaccines have low immunity to certain groups of people; repeated immunizations are required to produce better immunity; vaccine protection is prone to seriously decrease over time; There are no effective vaccines against infectious diseases

Method used

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  • Immune enhancer for pig vaccine, and preparation method and application thereof
  • Immune enhancer for pig vaccine, and preparation method and application thereof
  • Immune enhancer for pig vaccine, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of recombinant porcine interferon α, recombinant porcine interferon γ, and recombinant porcine IL-2

[0037] The preparation of recombinant porcine interferon α, recombinant porcine interferon γ, and recombinant porcine IL-2 comprises the following steps:

[0038] 1) Preparation of recombinant porcine interferon α

[0039] Construction of engineering Escherichia coli containing recombinant porcine interferon α target gene → engineering bacteria fermentation → bacterial liquid crushing → centrifugation to obtain the supernatant containing the target protein → protein purification → intermediate product inspection; for the preparation method, please refer to the published on October 22, 2008 Chinese patent CN101289666.

[0040] 2) Preparation of recombinant porcine interferon gamma

[0041] Construction of engineering Escherichia coli containing recombinant porcine interferon gamma target gene → engineering bacteria fermentation → bacterial liquid crushing →...

Embodiment 2

[0067] Immunopotentiator for preparing porcine vaccine

[0068] The new immunopotentiator for porcine vaccines of this embodiment includes porcine genetically engineered cytokine complexes and lyoprotectants.

[0069] The porcine genetically engineered cytokine complex comprises the following components in parts by weight: 50 parts by weight of recombinant porcine interferon α, 25 parts by weight of recombinant porcine interferon γ, 25 parts by weight of recombinant porcine interferon α, and 25 parts by weight of recombinant porcine interferon α The nucleotide sequence of the coding gene is shown in SEQ ID No.1, the nucleotide sequence of the coding gene of the recombinant porcine interferon gamma is shown in SEQ ID No.2, and the coding of the recombinant pig IL-2 The nucleotide sequence of the gene is shown in SEQ ID No.3.

[0070] The lyoprotectant includes skimmed milk powder, glucose and serum albumin, and the concentration of each component in the immune enhancer is as f...

Embodiment 3

[0078] Immunopotentiator for preparing porcine vaccine

[0079] The new immunopotentiator for porcine vaccines of this embodiment includes porcine genetically engineered cytokine complexes and lyoprotectants.

[0080] The porcine genetically engineered cytokine complex comprises the following components in parts by weight: 60 parts by weight of recombinant porcine interferon α, 20 parts by weight of recombinant porcine interferon γ, 20 parts by weight of recombinant porcine IL-2, the recombinant porcine interferon α The nucleotide sequence of the coding gene is shown in SEQ ID No.1, the nucleotide sequence of the coding gene of the recombinant porcine interferon gamma is shown in SEQ ID No.2, and the coding of the recombinant pig IL-2 The nucleotide sequence of the gene is shown in SEQ ID No.3.

[0081] The lyoprotectant includes skimmed milk powder, glucose and serum albumin, and the concentration of each component in the immune enhancer is as follows: skimmed milk powder 5g...

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Abstract

The invention provides an immune enhancer for pig vaccine, and a preparation method and application thereof. The immune enhancer for pig vaccine comprises a pig gene engineering cell factor compound and a freeze-drying protective additive, wherein the pig gene engineering cell factor compound comprises the following ingredients in parts by weight: 50 to 70 parts of recombinant porcine IFN-Alpha, 15 to 25 parts of recombinant porcine IFN-Gamma and 15 to 25 parts of recombinant porcine IL-2. The immune enhancer has the following effects that after the combined use of the immune enhancer and the vaccine, the vaccine protective period specificity antibody level is improved; the antibody generating window period is shortened by 30 to 50 percent; the secretory volume of specific humoral immunity response markers 1L-4 can be improved by 1 to 5 times through being compared with that during the single use of the vaccine; the cellullar immunologic response level of the pig vaccine can be improved; the secretory volume of the cellullar immunologic response marker interferon Gamma is improved by 1 to 5 times through being compared with that during the single use of the vaccine; the safety is high; the immune enhancer can be prepared into a water soluble state; the problems that on the existing market, the vaccine immunologic adjuvant has the injection abscess, nodule and ulceration, so that the acute or chronic inflammation reaction and the like can be caused are solved.

Description

technical field [0001] The invention relates to an immunopotentiator for porcine vaccines, a preparation method and application thereof, and belongs to the technical field of veterinary biological products. Background technique [0002] Vaccine immunization is generally considered to be one of the most effective and economical means to prevent and control livestock and poultry infectious diseases. However, traditional vaccines and new vaccines have also encountered some intractable problems: vaccines have low immunity to certain groups of people; repeated immunizations are required to produce better immunity; vaccine protection is prone to seriously decrease over time; There are no effective vaccines against infectious diseases. Therefore, many vaccines, especially new vaccines such as subunit vaccines, DNA vaccines and peptide vaccines, must produce stronger and longer-lasting immune responses to meet the needs. Immunopotentiators can mobilize, regulate, and improve the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P37/04
CPCA61K39/39A61K2039/55522A61K2039/55533A61K2039/57A61K2039/575
Inventor 王明丽赵俊甘霖赵雨周炜符修乐李树启符先宝戚仕梅高耀辉赖鹏飞汪良青马腾飞王利利
Owner WUHU YINGTE FEIER BIOLOGICAL PROD IND RES INST CO LTD
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