Upland cotton transformation event ICR24001 and specificity identification method thereof

A cotton and transgenic technology, applied in the field of upland cotton transformation event ICR24001 and its specific identification, can solve the problems of small planting area, difficult aggregation of upland cotton yield traits, sea-island cotton fiber traits, and trait separation

Active Publication Date: 2016-12-07
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional breeding method uses hybridization to improve the fiber quality of cotton. Sea-island cotton is the representative cotton of high-quality long fiber and one of the four major cultivated varieties. However, because its output cannot match that of upland cotton, it is now planted in a relatively small area in the world. Few, from the genome level, sea island cotton and upland cotton are both allotetraploid, and interspecific hybridization can be carried out between them, but existing research shows that the offspring of the hybrids of the two have serious trait separation, and it is difficult to aggregate land cotton. Yield traits of cotton and fiber traits of sea-island cotton

Method used

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  • Upland cotton transformation event ICR24001 and specificity identification method thereof
  • Upland cotton transformation event ICR24001 and specificity identification method thereof
  • Upland cotton transformation event ICR24001 and specificity identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Embodiment 1, the acquisition of transgenic GhKCS2 cotton and the analysis of agronomic traits

[0087] 1. Obtaining of transgenic GhKCS2 cotton

[0088] 1. Construction of recombinant vector

[0089] The expression cassette A was inserted between the EcoRI and PstI restriction sites of the pCambia2300 vector (the plasmid was purchased from the BioVector Plasmid Vector Strain Cell Gene Collection Center under the NTCC Type Culture Collection Center, the article number is: Biovectorpcambia2300), and the pCambia2300 vector was maintained. Other sequences remained unchanged, and the recombinant vector (SEQ ID NO: 4) was obtained. The nucleotide sequence of the above expression cassette A is shown in the 234th-2974th nucleotides in Sequence Table 1. Expression cassette A comprises the promoter, GhKCS2 gene and NOS terminator from the CAMV35S of cauliflower mosaic virus successively from upstream; shown; the nucleotide sequence of the GhKCS2 gene is shown in the 1110-2699...

Embodiment 2

[0123] Example 2. Acquisition and identification of traits of ICR24001 breeding offspring and analysis of their agronomic traits

[0124] 1. Acquisition of traits in ICR24001 breeding offspring

[0125] 1. Hybridization

[0126] The pollen of the first cotton is removed by hand or human action in the afternoon before flowering, and the stigma of the flower is covered with a wax tube to prevent foreign pollen from contacting the stigma. When the pollen of the second cotton loosens in the next morning, pass Humans manually collect pollen from the second cotton and contact the pollen with the style or stigma of the first plant to complete the hybridization process. Wherein, when the first cotton is ICR24001 in the embodiment, the second cotton is other cotton, or the second cotton is ICR24001, and the first cotton is other cotton.

[0127] The hybrid bolls are harvested in the floc opening stage, and the cotton is naturally dried, ginned, and towed with concentrated sulfuric ac...

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Abstract

The invention discloses an upland cotton transformation event ICR24001 and a specificity identification method thereof. A GhKCS2 gene is overexpressed in upland cotton CCRI24 to obtain transgenic cotton ICR24001, an exogenous DNA fragment of the transgenic cotton is inserted into the 68405357th-68405382th locus interval of a No. A11 chromosome of a target cotton genome to replace 24 bp base sequences in the 68405357th-68405382th locus interval of the No. A11 chromosome so as to obtain cotton. It is proved through tests that the transgenic cotton ICR24001 is not only higher than an acceptor material in fiber length, specific strength, uniformity and seed cotton yield and is also higher than the acceptor material in plant height, leaf length, leaf width, petiole length, petiole width, fruit branch number, boll forming number, single boll weight, ginning outturn and the like, and a foundation is laid for research of cultivation of the transgenic cotton having excellent fiber quality.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an upland cotton transformation event ICR24001 and a specific identification method thereof. Background technique [0002] The transformation event is a molecular structure composed of the upstream and downstream flanking regions of the exogenous gene at the genome insertion site and the exogenous gene. Usually, a plant transformed with an exogenous gene can obtain a population of transformants comprising a large number of independent events, each of which is unique. The expression of exogenous genes in plants is influenced by the chromosomal location where the exogenous genes are inserted. This may arise from the influence of chromatin structure or transcriptional regulatory elements near the integration site. The expression levels of the same gene in different transformation events can vary widely, and there may also be differences in the spatial or temporal patterns ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/11C12Q1/68A01H5/00A01H1/02
Inventor 李付广秦文强杨召恩张朝军武芝侠张雪妍杨作仁鲁丽丽王晔王倩华孔德培龚骞王玉芬
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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