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Virus-free rapid propagation method of purple sweet potato Zhezi No. 1

A purple sweet potato, No. 1 technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of rare treatment methods, achieve the effects of eliminating adverse reactions, shortening the detoxification culture time, and improving the detoxification efficiency

Inactive Publication Date: 2016-12-14
TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, the general method of detoxification and rapid propagation of Zhezi No. 1 purple sweet potato adopts the method of shoot apex meristem culture or heat treatment for detoxification and rapid propagation, which has a certain effect. The treatment method of poisonous rapid multiplication is rare

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A virus-free and rapid propagation method of Zhezi No. 1 purple sweet potato, the specific steps are:

[0018] (1) Stripping of the shoot tip of Zhezi No. 1 purple potato seedlings:

[0019] Cut out the top buds of purple sweet potato seedlings with a length of 0.6cm under sterile conditions, remove the leaves visible to the naked eye, and then peel off the cones of the shoot apex meristem with a length of 0.5mm under the observation of a solid microscope, and inoculate them into the shoot apex for induction culture On the base, the culture medium is packed in a 100mm petri dish, and the capacity of each dish is 25ml, and each petri dish is inoculated with 3 shoot tips;

[0020] (2) Virus-free culture of purple potato seedling shoot tips of Zhezi No. 1:

[0021] In the artificial climate box, the shoot tips inoculated on the medium were subjected to variable temperature heat treatment culture, and the treatment time was 31 days; the variable temperature heat treatment ...

Embodiment 2

[0028] A virus-free and rapid propagation method of Zhezi No. 1 purple sweet potato, the specific steps are:

[0029] (1) Stripping of the shoot tip of Zhezi No. 1 purple potato seedlings:

[0030] Under sterile conditions, cut the top buds of purple potato seedlings with a length of 1 cm, remove the leaves visible to the naked eye, and then peel off the cones of the shoot apex meristem with a length of 0.9 mm under the observation of a solid microscope, and inoculate them into the shoot apex induction medium Above, the culture medium is packed in 100mm petri dishes, each dish has a capacity of 25ml, and each petri dish is inoculated with 3 shoot tips;

[0031] (2) Virus-free culture of purple potato seedling shoot tips of Zhezi No. 1:

[0032] In the artificial climate box, the shoot tips inoculated on the medium were subjected to variable temperature heat treatment culture, and the treatment time was 32 days; the variable temperature heat treatment culture included dark tre...

Embodiment 3

[0039] A virus-free and rapid propagation method of Zhezi No. 1 purple sweet potato, the specific steps are:

[0040] (1) Stripping of the shoot tip of Zhezi No. 1 purple potato seedlings:

[0041] Under sterile conditions, cut out the top buds of purple sweet potato seedlings with a length of 0.8 cm, remove the leaves visible to the naked eye, and then peel off the cones of the shoot apex meristem with a length of 0.7 mm under the observation of a solid microscope, and inoculate them into the shoot tip for induction culture On the base, the culture medium is packed in a 100mm petri dish, and the capacity of each dish is 25ml, and each petri dish is inoculated with 3 shoot tips;

[0042] (2) Virus-free culture of purple potato seedling shoot tips of Zhezi No. 1:

[0043] In the artificial climate box, the shoot tips inoculated on the medium were subjected to variable temperature heat treatment culture, and the treatment time was 31 days; the variable temperature heat treatmen...

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Abstract

The invention relates to a virus-free rapid propagation method of purple sweet potato Zhezi No. 1, comprising the specific steps of (1), stripping stem-tip merismatic cones from purple sweet potato seedlings under aseptic condition, and inoculating to a stem-tip induction medium; (2), in an artificial climate box, subjecting the step tips inoculated to the medium to variable temperature thermal treatment and cultivation and then to cultivation in a normal environment; (3), selecting robust purple sweet potato aseptic seedlings, cutting to obtain stem segments with uniform size, axillary buds and zero root knots, inoculating to a multiplication medium, culturing in the artificial climate box, selecting rooted purple sweet potato seedlings, exercising them in a greenhouse, washing the medium at roots, and transplanting to sandy loam for growth. In detoxification of purple sweet potato Zhezi No. 1, stem-top culture and variable temperature thermal treatment are combined, virus-free culture time is shortened, detoxification efficiency is improved, detoxification technology for purple sweet potato Zhezi No. 1 is optimized, and a stable and efficient technical system for virus-free rapid propagation of purple sweet potato Zhezi No. 1 is established.

Description

technical field [0001] The invention relates to the field of detoxification of purple sweet potatoes, in particular to a method for detoxification and rapid propagation of No. 1 purple sweet potato. Background technique [0002] Zhezi No. 1 purple potato is different from ordinary sweet potatoes. It is not only rich in functional ingredients such as vitamins, anthocyanins and selenium, but also has anti-cancer, anti-bacterial, anti-oxidation, anti-hyperglycemic, anti-mutant, enhanced memory and improved liver function. It is a natural food that integrates medicinal, health care and edible. Zhezi No. 1 purple sweet potato is sought after and concerned by consumers and researchers because of its high health value, convenient source and low cost. It is an economic crop with strong application value and development prospects. Zhezi No. 1 purple sweet potato is asexually propagated with vegetative organs such as tubers and stems. During the process of introduction, reproduction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 王立国郭子江张嘉李建军朱永伶芮淑会王鸿鸣李彦阳
Owner TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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