Application of ascaroside deltaC7 in control of Monochamus ahernatus Hope and interception of pine wood nematode propagation

A technology of Monochamus alternatus and pine xylophilus, applied in application, nematicides, insecticides, etc., can solve problems such as inability to emerge, delay in emergence time of Monochamus alternatus, inability to form and cut off propagation and diffusion, etc., to achieve Easy to promote and apply, obvious control effect, simple operation

Inactive Publication Date: 2016-12-21
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, interfering with and regulating the development of Monochamus alternatus larvae can delay or prevent the eclosion of Monochamus alternatus, thus making L. Ⅳ Unable to form and cut off the way of its spread

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In the area where B. xylophilus occurs, before the pupation of Monochamus alternatus begins at the end of April and the beginning of May, 40 Pinus massoniana plants containing multiple monochamus alternatus larvae were selected. The identification method is as follows: pine trees with pine wood nematode damage do not flow fat after drilling, there are traces of monochamus alternatus spawning grooves, and some pine needles lose green and yellow. 20 strains are used as the experimental group, and 20 strains are used as the control group. On the sunny side of the tree body, the xylem monochamus alternata bores into the hole with an electric drill to drill a hole with a diameter of 1 cm and a depth of 2 cm, which communicates with the pupal chamber of the monochamus alternatus larvae. The dose of ascaroside △C7 was directly added to the wells of the experimental group at a dose of 6nM / well, and the dosage of ascaroside △C7 was 30nM / m 3 Pine tree body. In the control group,...

Embodiment 2

[0023] Ascaroside ΔC7 was dissolved in methanol and adsorbed on a rubber septa (0.5 cm diameter, 1 cm length). In the area where B. xylophilus occurs, 40 Pinus massoniana plants containing multiple monochamus larvae were selected. The identification method is as follows: pine trees with pine wood nematode damage do not flow fat after drilling, there are traces of monochamus alternatus spawning grooves, and some pine needles lose green and yellow. 20 strains are used as the experimental group, and 20 strains are used as the control group, and holes with a diameter of 1 cm and a depth of 2 cm are drilled with an electric drill in the xylem of the tree body on the sunny side of the tree. In the experimental group, a number of silicone rubber plugs containing 1 μl of ascaroside C9 were scattered and inserted into the holes (after mixing 5 μl of ascaroside △C7 and 100 μl of methanol, take 20 μl and drip it into the silicone rubber plug, and use it after the solvent evaporates), and...

Embodiment 3

[0025] Ascaroside △C7 was dissolved in ethanol and adsorbed on a silicone rubber stopper (0.5 cm diameter, 1 cm length). In the area where B. xylophilus occurs, 40 Pinus massoniana plants containing multiple monochamus larvae were selected. The identification method is as follows: pine trees with pine wood nematode damage do not flow fat after drilling, there are traces of monochamus alternatus spawning grooves, and some pine needles lose green and yellow. 20 strains are used as the experimental group, and 20 strains are used as the control group, and holes with a diameter of 1 cm and a depth of 2 cm are drilled with an electric drill in the xylem of the tree body on the sunny side of the tree. In the experimental group, a number of silicone rubber plugs containing ascaroside △C7 were scattered and inserted into the holes (take 20 μl of 3 μM ascaroside △C7 ethanol solution and drop it into the silicone rubber plug, and it can be used after the solvent evaporates). The dosage o...

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Abstract

The invention relates to an application of ascaroside deltaC7 in control of Monochamus ahernatus Hope and interception of pine wood nematode propagation. A method for controlling Monochamus ahernatus Hope by using ascaroside deltaC7 is characterized in that the ascaroside deltaC7 is placed in pine trees containing larvae of Monochamus ahernatus Hope. The method can be used in control of woodland Monochamus ahernatus Hope and treatment of pine wood nematode infected pine trees, and can solve the problems of large quantity of Monochamus ahernatus Hope populations, heavy damages and rapid diffusion of pine wood nematodes by Monochamus ahernatus Hope in the outbreak site of the pine wood nematodes.

Description

technical field [0001] The invention relates to the application of ascaroside ΔC7 in the prevention and control of Monochamus alternatus and the transmission of pine wood nematode. Background technique [0002] Pine wood nematode disease, known as the "cancer" of pine trees, is the most dangerous forest disease in the world. It is a devastating disease of pinaceae plants. The host plant can die within 2-3 months after being infected with the disease, and 3- The entire pine forest can be destroyed within 5 years, which is extremely harmful. Since the first discovery of pine wood nematode disease in Nanjing in 1982 in my country, the epidemic has spread rapidly and spread to 14 provinces and cities including Jiangsu, Anhui, Guangdong, Guangxi, Zhejiang, Fujian, Shanghai, Chongqing, Hubei, Shandong, and 86 counties. . In the past 28 years, more than 500 million pine trees have been killed, more than 5 million mu of pine forests have been destroyed, and hundreds of billions of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P7/04A01P5/00
CPCA01N43/16
Inventor 赵莉蔺孙江华
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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