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A kind of culture medium and culture method for prolonging the preservation period of banana tissue culture seedlings

A culture method and culture medium technology, which are applied in the field of culture medium and culture to prolong the storage period of banana tissue culture seedlings, and achieve the effects of strong operability, simple and effective method, and safe and reliable production

Active Publication Date: 2018-10-02
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of culture medium and culture method that prolongs the shelf life of banana tissue culture seedlings, to solve the problem of off-season and peak season encountered in the annual production of banana tissue culture plantlets production factory, realize the annual balanced production of banana tissue culture seedlings , it can not only produce normally in the off-season, but also avoid overload production in the peak season, which is convenient for production management and improves production efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Material: The experimental material is banana (Musa nana Lour.) adventitious bud propagation material.

[0017] The sucking buds of bananas were selected as explants, and after routine disinfection, the terminal buds were inoculated on the adventitious bud induction medium, cultured for about 45 days to obtain the adventitious buds, and the adventitious buds were cut and inoculated on the proliferation medium to obtain a large number of banana adventitious buds. Bud propagation material.

[0018] 2. Drug purchase

[0019] Buy from related companies the medicines for the ingredients of MS medium required for the production of banana tissue culture seedlings, including macroelements, trace elements, organic matter, agar, etc., and purchase plant growth regulators indolebutyric acid (IBA) and naphthalic acid (NAA) As well as medicines that extend the shelf life of the ingredients in the mother liquor.

[0020] 3. Mother liquor preparation

[0021] The formula of bana...

Embodiment 2

[0031] Embodiment 2 is basically the same as Embodiment 1, the difference is:

[0032]The formula of the banana rooting medium in embodiment 2 is: take MS medium as basic medium, also contain IBA2.0mg / L, NAA0.2mg / L, sucrose 30g / L, agar 7g / L, pH 6.0. The formula for extending the shelf life mother liquor is: containing ammonium nitrate 16.5g / L, potassium nitrate 19.0g / L, potassium dihydrogen phosphate 2.0g / L, magnesium sulfate heptahydrate 3.5g / L and calcium chloride 3.5g / L. In the medium for prolonging the shelf life of banana tissue culture plantlets per liter of preparation, it added 100ml of mother liquor for extending the shelf life on the basis of the banana rooting medium. That is, the medium for prolonging the storage period of banana tissue culture seedlings is based on the banana rooting medium, and then add ammonium nitrate 1.65g / L, potassium nitrate 1.90g / L, potassium dihydrogen phosphate 0.20g / L, magnesium sulfate heptahydrate 0.35g / L and calcium chloride 0.35g / L,...

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Abstract

The invention discloses a culture medium and culture method for prolonging the storage life of banana tissue culture seedlings. The culture medium for prolonging the storage life of the banana tissue culture seedlings is obtained by adding ammonium nitrate of 1.50 g / L-1.65 g / L, potassium nitrate of 1.80 g / L-1.90 g / L, monopotassium phosphate of 0.15 g / L-0.20 g / L, magnesium sulfate heptahydrate of 0.3 g / L-0.35 g / L and calcium chloride of 0.3 g / L-0.35 g / L on the basis of a banana rooting culture medium. The culture medium is inoculated with banana adventitious buds to be subjected to rooting culture, the storage life of the banana tissue culture seedlings can be prolonged, and annual balanced production of the banana tissue culture seedlings is achieved. The culture medium has the advantages of being simple, easy to operate, safe and reliable, and the adopted added ingredients cannot generate toxic or side effect to the cultured material; the method is simple and effective and has the advantages of being high in operability, production efficiency and application value and the like.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium and a culture method for prolonging the storage period of banana tissue culture seedlings. Background technique [0002] Plant tissue culture has been widely used in many fields of agricultural production, and has brought profound impact to modern agriculture in terms of seedling detoxification production, rapid propagation of seedlings, large-scale application of new plant resources, and breeding of new plant varieties. It is a model of the application of agricultural biotechnology in modern agriculture and an important part of the modern seed industry. At present, plant tissue culture seedlings in my country have been widely promoted and applied in flowers, fruit trees, shade plants, medicinal plants, etc. The annual trade volume of plant tissue culture seedlings in the world has exceeded 15 billion U.S. dollars, and every year Speeds o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴坤林曾宋君张建霞郑枫段俊
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI