A method for identifying the sex and gonad maturity of Apostichopus japonicus

A technology of imitating sea cucumber and maturity, applied in application, climate change adaptation, fish farming, etc., can solve the problem of increasing labor and production cost of artificial ripening, increasing economic loss of artificial breeding of sea cucumber, and living sex table of sea cucumber. Problems such as difficulty in observation and identification, to achieve the effects of good operability, reduced parent loss, and technical mastery

Inactive Publication Date: 2019-03-29
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the difficulty in the apparent sex identification of living sea cucumbers and the difficulty in distinguishing males and females, it is necessary to cultivate and mature a large number of parent bodies to ensure the breeding effect when artificially breeding sea cucumbers. Artificial cleaning of fertilized eggs; if there are many females, the fertilization rate will be low and the emergence rate will be reduced, which will greatly increase the labor and production costs in the artificial maturation process
At the same time, the gonad maturity is difficult to judge, and the method of dissecting the parent body is used to observe the gonad maturity of sea cucumber, which increases the economic loss in the process of artificial breeding of sea cucumber

Method used

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  • A method for identifying the sex and gonad maturity of Apostichopus japonicus
  • A method for identifying the sex and gonad maturity of Apostichopus japonicus
  • A method for identifying the sex and gonad maturity of Apostichopus japonicus

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Negative pressure tissue harvesters, such as figure 1 As shown, it includes a push rod 1, a piston 2, a cylinder 3, a fixed sleeve 4, a collection tube 5, an outer sleeve 6 and a collection tube protection sleeve 7; wherein the push rod 1 and the piston 2 are connected and placed in the cylinder 3, The collection tube 5 is a side suction type, one end of the collection tube is sharp, and there is a small hole 8 on one side of the sharp end of the collection tube, and the lower edge of the small hole is a sharp blade structure; The sharp end of the tube is far away from the cylinder, the outer sleeve 6 is wrapped around the outside of the collection tube 5, and the outer sleeve can slide up and down along the collection tube, and the collection tube protection sleeve 7 is located outside the outer sleeve 6 to protect the collection tube 5 from external force damage. The effect of the collection tube specification of the negative pressure tissue harvester used on the sex ...

Embodiment 2

[0025] Example 2 Effects of Different Development Stages on Sex Identification of Apostichopus japonicus

[0026] Utilize the method of the present invention (acquisition tube specification outer diameter 0.81mm, internal diameter 0.5mm), in November 2015, March, May and August 2016 respectively to the sea cucumber (256 ± 15g) in Weihai, Yantai culture pond Gender was identified, and 30 relatives were taken at each time. Record the identification results, dissect the sea cucumber after the identification, and verify the experimental results. The experimental results are shown in Table 2.

[0027] Table 2 Effects of different developmental stages on sex identification of Apostichopus japonicus

[0028]

[0029] According to the experimental results, after repeated certifications, the method of the present invention has an accuracy rate of 100% for sex identification of the developed gonads of the japonicus japonicus.

Embodiment 3

[0030] Example 3 Effects of Different Collection Locations on Sex Identification of Apostichopus japonicus

[0031] Utilize the method of the present invention (acquisition tube specification outer diameter 0.81mm, inner diameter 0.5mm), identify the sex of sea cucumber from the three positions of the dorsal front, middle and rear of the gonad of sea cucumber respectively. Collect the gonads from the dorsal side toward the anus at the middle part of the side, that is, 2-3cm behind the gonads of the japonicus japonicus. Each collection site detects 30 japonicus japonicus with a size of 236±27g. Record the experimental results and dissect the japonicus japonicus after the identification , to verify the experimental results, the experimental results are shown in Table 3.

[0032] According to the experimental results, after repeated certifications, when the method of the present invention is used to identify the sex of the sea cucumber, the collection from the dorsal middle of th...

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Abstract

The invention discloses an identification method for the sex and the gonad maturity degree of apostichopus japonicus, and belongs to the technical field of aquaculture. Parts of gonad tissue of anesthetized apostichopus japonicus are obtained through a negative-pressure tissue collector, the sex and the gonad maturity degree of apostichopus japonicus are identified according to the colors of female and male glands of apostichopus japonicus and the species, the size, the quantity and the structural characteristics of germ cells of female and male glands of apostichopus japonicus. The sex and the gonad maturity degree of apostichopus japonicus are identified with the method, a trauma is small, apostichopus japonicus parents do not need to be dissected, and losses of parents and the economic losses are reduced.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for identifying the sex and gonad maturity of sea cucumbers. Background technique [0002] Imitation sea cucumber, also known as sea cucumber, is one of the best quality among more than 20 kinds of edible sea cucumbers in China. It is listed as one of the "Eight Treasures of Seafood" and has been widely cultivated in my country. It is necessary to identify the male and female sex and gonad maturity of sea cucumbers in the process of artificial breeding of sea cucumbers, breeding of fine varieties and scientific experiments. However, there is no significant difference in appearance between male and female of A. japonicus, and the maturity of gonads cannot be identified from the appearance. Therefore, the identification of sex and gonad maturity of A. japonicus is a difficult point in the process of artificial breeding of A. japonicus. In the past, the sex...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/30A01K61/90
CPCY02A40/81
Inventor 李凤辉陈四清卢斌孙慧玲刘长琳谭杰
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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