Tumor microvascular self-assembly generation system

Abstract

The invention discloses a tumor microvessel self-assembly generation system. The system comprises a tumor microsphere column and a pulsation system; the tumor microsphere column comprises a perfusion column and tumor microspheres located in the perfusion column, and the two ends of the perfusion column are each provided with a micropore filter membrane; the pulsation system comprises a peristaltic pump, a pulse piston pump, a rotating culture support, a liquid storage bottle, an oxygenator, a filter and a buffer bottle; the perfusion column, the liquid storage bottle, the oxygenator and the buffer bottle are sequentially communicated end to end to form a circulating channel. In the working process, the peristaltic pump is started to start to conduct perfusion, a fluid in the buffer bottle forms fluid circulating through a first pulse pipeline, when the pulse piston pump conducts extraction operation, more fluids can swarm into the perfusion column in a pulsed mode to form a pulsed fluid to pass through the perfusion column, then a pulsed fluid caused by cardiac impulse is simulated, and fluid flow and fluid shear force are applied to the tumor microspheres in the perfusion column; oxygen is supplied and carbon dioxide is removed by combining the oxygenator which simulates a lung air exchange device, and then occurrence of the tumor microvessels in a body is finally simulated.

Description

technical field

[0001] The invention belongs to the field of medical experiments, in particular to a tumor microvessel self-assembly generation system. Background technique

[0002] The growth of solid tumors is vascular-dependent, and tissues with a diameter of more than 2 mm require blood vessels to supply nutrients. The same is true for angiogenesis in brain tumors. Studying the angiogenesis mechanism of brain tumors is the focus and difficulty of studying tumor progression and antitumor drugs targeting tumor blood vessels. The difficulty is that there is no ideal model of tumor angiogenesis in vitro. Contents of the invention

[0003] The object of the present invention is to provide a tumor microvessel self-assembly generation system, which can solve at least one of the above-mentioned problems.

[0004] According to one aspect of the present invention, a tumor microvessel self-assembly generation system is provided, including a tumor microsphere column and a pulsa...

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