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Method for extracting ginkgo seed bioactive polypeptides

A technology of biologically active polypeptides and ginkgo, which is applied in the field of extracting biologically active polypeptides from ginkgo, can solve the problems of destroying the physiological activity of polypeptides, unstable polypeptide structures, acid-base and other chemical pollution, etc., so as to improve the extraction rate, facilitate human body absorption, increase The effect of skin nutrition

Active Publication Date: 2017-01-04
徐州银杏源生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for extracting ginkgo biologically active polypeptides, which solves the damage caused by the chemical extraction method to the polypeptides, resulting in unstable polypeptide structures, destroying the physiological activity of polypeptides, producing toxic by-products, and chemical pollution such as acid and alkali. question

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0029] A method for extracting ginkgo bioactive polypeptides, comprising the following steps:

[0030] Step 1, crushing: boil the ginkgo kernels in boiling water for 5 minutes, use a Chinese herbal medicine grinder to crush the ginkgo kernels after drying, and pass through a 200-mesh sieve to obtain 200-mesh ginkgo powder;

[0031] Step 2, enzymatic hydrolysis: dissolve the ginkgo powder in step 1 with water, wherein the solid-to-liquid ratio of ginkgo powder to water is 1g:20mL, stir and mix well, adjust the pH to 5.5, add wall-breaking enzyme and pectin while stirring Enzyme, cellulase and protease, enzymatic hydrolysis at 55°C for 4 hours to obtain an enzymatic hydrolysis solution, and then subject the enzymatic hydrolysis solution to inactivation of enzymes at high temperature to obtain an enzyme inactivation solution;

[0032] Among them, the method of inactivating the enzyme at high temperature is: incubating the enzymatic hydrolysis solution at 100°C for 5 minutes;

[...

Embodiment 2

[0041] A method for extracting ginkgo biologically active polypeptides, comprising the following steps:

[0042]Step 1, crushing: boil the ginkgo kernels in boiling water for 4 minutes, and use an ultrafine pulverizer to crush the ginkgo kernels after drying, and pass through a 500-mesh sieve to obtain 500-mesh ginkgo powder;

[0043] Step 2, enzymatic hydrolysis: dissolve the ginkgo powder in step 1 with water, wherein the solid-to-liquid ratio of ginkgo powder to water is 1g:25mL, stir and mix well, adjust the pH to 6, add wall-breaking enzyme and pectin while stirring Enzyme, cellulase and protease, enzymatic hydrolysis at 55°C for 3 hours to obtain an enzymatic hydrolysis solution, and then subject the enzymatic hydrolysis solution to inactivation of enzymes at high temperature to obtain an enzyme inactivation solution;

[0044] Among them, the method of inactivating the enzyme at high temperature is: incubating the enzymatic hydrolysis solution at 95°C for 5 minutes;

[...

Embodiment 3

[0053] A method for extracting ginkgo bioactive polypeptides, comprising the following steps:

[0054] Step 1, crushing: boil the ginkgo kernels in boiling water for 3 minutes, and use a Chinese herbal medicine grinder to crush the ginkgo kernels after drying, and pass through a 1000-mesh sieve to obtain 1000-mesh ginkgo powder;

[0055] Step 2, enzymatic hydrolysis: dissolve the ginkgo powder in step 1 with water, wherein the solid-to-liquid ratio of ginkgo powder to water is 1g:30mL, stir and mix to adjust the pH value to 7.0, add wall-breaking enzyme and pectin while stirring Enzyme, cellulase and protease, enzymatic hydrolysis at 58°C for 1 hour to obtain an enzymatic hydrolysis solution, and then subject the enzymatic hydrolysis solution to inactivation of enzymes at high temperature to obtain an enzyme inactivation solution;

[0056] Among them, the method of inactivating the enzyme at high temperature is: incubating the enzymatic hydrolysis solution at 100°C for 7 minut...

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Abstract

The invention discloses a method for extracting ginkgo seed bioactive polypeptides. The method comprises the following steps: pulverization: pulverizing to obtain ginkgo seed powder; enzymolysis: dissolving the ginkgo seed powder in water, uniformly stirring and mixing, regulating the pH value, adding a wall-breaking enzyme, pectinase, cellulase and proteinase while stirring, carrying out enzymolysis to obtain an enzymolysis solution, and carrying out enzyme inactivation by high temperature to obtain an enzyme inactivation solution; two-step extraction: regulating the temperature of the enzyme inactivation solution to 3-5 DEG C, keeping for 1-3 hours, hydrolyzing, separating to obtain a primary supernate and a primary residue, carrying out water extraction and separation on the primary residue, and collecting a secondary supernate; concentration: merging the primary and secondary supernates, and concentrating to obtain a concentrated solution; and drying: drying the concentrated solution to obtain the ginkgo seed bioactive polypeptides. The method disclosed by the invention prevents the active polypeptide structure from being damaged, and effectively enhances the extraction rate. The molecular weight of most small-molecule active polypeptides is less than 1000Da, so the bioactive polypeptides can be absorbed by the human body more easily.

Description

technical field [0001] The invention belongs to the technical field of ginkgo product processing, and in particular relates to a method for extracting ginkgo bioactive polypeptides. Background technique [0002] Biologically active peptides are short peptide compounds that have recently been found to have optimized functions for cell functions or metabolic activities of living organisms. Compared with full-length proteins, the excellent physical and chemical properties of biologically active peptides, such as good solubility, hydrophilicity, low viscosity, low foaming, etc., all make the bioavailability of active peptides higher, even higher than that of amino acids. It is easy to absorb and has the effect of regulating the physiological functions of the human body. It has some biological activities that the original protein or its component amino acids do not have. The biological functions of bioactive peptides include immune regulation, anti-hypertension, antibacterial, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14
CPCC07K1/145C12P21/06
Inventor 范文杰孙俊松
Owner 徐州银杏源生物工程有限公司
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