Unlock instant, AI-driven research and patent intelligence for your innovation.

Multiple myeloma drug resistance gene mutation detection kit and application method

A multiple myeloma detection kit technology, applied in the fields of medicine and molecular biology, can solve the problem of being unable to inhibit the self-ubiquitination of CRBNYW/AA, and achieve clear detection results, simple detection process, and strong purpose Effect

Inactive Publication Date: 2017-01-04
北京海思特医学检验实验室有限公司
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mutation of two key amino acid residues of CRBN (Y384A / W386A double mutation, denoted as CRBNYW / AA) can avoid the binding of Thalidomide but retain the self-ubiquitination activity, and Thalidomide cannot inhibit the self-ubiquitination of CRBNYW / AA

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multiple myeloma drug resistance gene mutation detection kit and application method
  • Multiple myeloma drug resistance gene mutation detection kit and application method
  • Multiple myeloma drug resistance gene mutation detection kit and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0078] The specific implementation is as follows:

[0079] In step S1, the blood of the patient is collected as the sample to be tested, and CD138 magnetic beads are used to sort and screen positive lymphoma cells. Genomic DNA can be extracted using a conventional kit column extraction method and operated according to the instructions of the kit used to collect 50 μL A DNA solution with a concentration of 50-200ng / μL can be used directly for detection or stored at -20°C;

[0080] In step S2, a solution is prepared using the specific primers in the kit, wherein the concentration of the specific primers is 5 pmol / μL;

[0081] The amplification system of PCR amplification is shown in Table 3 below:

[0082] components Volume (μL) Template DNA 1 PCR Mix Reagent 12.5 upstream primer 1 downstream primer 1 Deionized water 9.5 total capacity 25

[0083] Common PCR instrument can be used for PCR amplification, and the reaction proce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a multiple myeloma drug resistance gene mutation detection kit and an application method and aims at obtaining new genetic support for multiple myeloma target treatment medication by conducting mutation detection on eight genes, namely CRBN, DDB1, CUL4B, IRF4, NR3C1, XBP1, PSMG2 and PSMB5, relevant to multiple myeloma drug resistance. The invention provides the kit which comprises reagents of specific primers of the eight genes and a detection method in which the kit is applied. Comprehensive and accurate detection can be conducted according to high-mutation sites of all the genes, and the detection result has strong guiding significance for clinics. The kit can be used for rapid and precise treatment of multiple myeloma, avoid meaningless treatment medication and play a very important role in the field of medical detection.

Description

technical field [0001] The invention relates to the technical fields of medicine and molecular biology, in particular to a kit for detecting multiple myeloma drug resistance-related gene mutations, and also relates to an application method of the kit in the detection of the above-mentioned gene mutations. Background technique [0002] Multiple myeloma (Multiple Myeloma, MM) is a plasma cell cancer characterized by the infiltration of abnormal plasma cells in the bone marrow. It is the most common blood cancer, accounting for about 1% of all cancers. It ranks second among common hematologic malignancies. Although new treatment methods have achieved great results, the prognosis of myeloma patients has been significantly improved, and the average survival time of patients has been extended from the past 3 to 4 years to 5 to 7 years, but myeloma is still an incurable tumor. [0003] The pathogenesis of MM is the mutation of genetic genes during the differentiation of B lymphocy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q1/6858C12Q2600/106C12Q2600/156C12Q2531/113C12Q2563/107C12Q2565/125
Inventor 傅廷娇陈欣张娟李悠南程宇王绪华梁超陈忠黄士昂
Owner 北京海思特医学检验实验室有限公司