Determination method for activity of superoxide dismutase in tobacco leaves damaged by pesticides

A technology of dismutase activity and superoxide, applied in the field of determination of superoxide dismutase activity, can solve the problems of inaccurate determination, less understanding of SOD influence and change, etc., and achieve the effect of accurate measurement

Inactive Publication Date: 2017-01-04
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention proposes a method for measuring the superoxide dismutase activity of tobacco leaves infringed by pesticides, which solves the problem of the lack of understanding of the influence and change of SOD in tobacco leaves infringed by pesticides (quinclorac) in the prior art, and the determination of not precise enough

Method used

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  • Determination method for activity of superoxide dismutase in tobacco leaves damaged by pesticides
  • Determination method for activity of superoxide dismutase in tobacco leaves damaged by pesticides
  • Determination method for activity of superoxide dismutase in tobacco leaves damaged by pesticides

Examples

Experimental program
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Embodiment

[0034] Tobacco seeds use the variety K326. Greenhouse sowing, seedling cultivation, transplanting at the stage of five to six true leaves, and measuring the SOD activity of pesticide-infested tobacco leaves

[0035] (1) Reagent preparation

[0036] 0.05mol L-1 phosphate buffer (pH7.8);

[0037] Extraction medium: 50mmol L-1 pH7.8 phosphate buffer (containing 1% polyvinylpyrrolidone);

[0038] 130mmol·L-1 methionine (Met) solution: Weigh 1.399gMet, dissolve with phosphate buffer and dilute to 100mL;

[0039] 750μmol L-1 NBT: Weigh 0.06133g NBT, dissolve it with phosphate buffer solution and dilute to 100mL, and store in the dark;

[0040] 100μmol L-1EDTA-Na2: Weigh 0.037224g EDTA-Na2, and make it to 1000mL;

[0041] 20μmol·L-1 riboflavin solution: weigh 0.0075g riboflavin, dilute to 100mL, and store in the dark

[0042] (2) Preparation of enzyme solution

[0043] Take the fourth unfolded tobacco leaf, remove the midrib, accurately weigh 0.3g (three parts in total) into a ...

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Abstract

The invention provides a determination method for the activity of superoxide dismutase in tobacco leaves damaged by pesticides. The determination method comprises the following steps that 1, reagents are prepared, wherein the phosphate buffer, the extracting medium, the methionine solution, NBT, EDTA-Na2 and the riboflavin solution are prepared; 2, an enzyme solution is prepared from the tobacco leaves; 3, the activity of a superoxide dismutase sample is determined; 4, a tobacco is transplanted into pesticidal soil of which the quinclorac adding concentrations are 1.04*10<-3> mg / kg, 2.08*10<-3> mg / kg, 4.17*10<-3> mg / kg, 8.33*10<-3> mg / kg and 1.67*10<-2> mg / kg in the period that the tobacco has 5-6 true leaves, and the activity of the superoxide dismutase in the tobacco leaves is measured on the 7 day, the 14 day, the 21 day, the 28 day and the 35 day by adopting the step 1 to the step 3 separately. Accordingly, the problems that in the prior art, few influences that the SOD is subjected to and few changes of the SOD in the tobacco leaves damaged by quinclorac are known, and determination is not precise enough are solved.

Description

technical field [0001] The invention relates to the field of chemical detection and analysis, in particular to a method for measuring the activity of superoxide dismutase in tobacco leaves damaged by pesticides. Background technique [0002] Quinclorac is an effective selective herbicide for controlling barnyardgrass in paddy fields. It is a hormone-type quinoline carboxylic acid herbicide. The symptoms of weed poisoning are similar to those of auxins. It is mainly used to control barnyard grass and has a long application period. 1-7 leaf stages are effective. Rice is safe. [0003] Relevant studies have shown that quinclorac, as a hormone herbicide, can stimulate the accumulation of ethylene in plants. The target site of quinclorac is located in the biosynthesis of the cell wall, and it acts as a cell wall synthesis inhibitor in grasses, and the sensitivity of different grasses' action sites to quinclorac. Ethylene can inhibit the activity of cell wall synthetase and thu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/3103G01N21/78
Inventor 陈泽鹏
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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