Nanosilver-coated polyethylene glycol and gelatin hydrogel impregnated polyurethane nanofibrous materials
A polyethylene glycol ester and polyethylene glycol technology, applied in the field of polyurethane nanofibrous omentum and abdominal hernia repair, can solve the problems of implantation failure of repair materials and hinder the development of abdominal hernia repair mesh
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Embodiment 1
[0037] Embodiment 1: the preparation of PU nanofiber mesh
[0038] First, 750 mg of polyurethane (PU) was dissolved in 5 ml of DMF. After dissolution, the solution was placed in a 10 ml syringe equipped with a syringe pump (PH 2000 Infusion). The positive lead from a high voltage (20KV) power supply (GAMMA, High Voltage Research) was then connected to the pin via an alligator clip. On the other side, a 15 x 15 cm stainless steel mesh was used to collect the polyurethane nanofibers. The distance from the needle to the omentum was 15 cm. Iron gauze is grounded for safety. The pump rate of the PU solution was set at 1 ml / h.
Embodiment 2
[0039] Example 2: Preparation of Methacrylated Gelatin (MA-G)
[0040] 1 g of gelatin was dissolved in 10 ml of PBS solution at 50° C.; then, 1 ml of methacrylic acid anhydride was added to the gelatin solution, and reacted at 50° C. for 1 hour with stirring. The product MA-G was then obtained by dialysis against double distilled water for 3 days and lyophilization.
Embodiment 3
[0041] Example 3: Preparation of nanosilver-coated, hydrogel-impregnated polyurethane omentum
[0042] A 3*3cm PU membrane with a thickness of 50 μm was immersed in a solution of 100 μl MA-gelatin (50 mg / ml) and 4 μl APS (200 mg / ml) for 15 seconds, and the other PU membrane was immersed in 100 μl PEGda (200 mg / ml). ml) and 0.5 μl of TEMED for 15 seconds. Then, one piece of PU nano-omentum was stacked on another; PEGda and MA-gelatin were gelled under the catalysis of APS and TEMED, and the two PU omentums were glued together. Then, the PU omentum was immersed in a dopamine solution (2 mg / ml in 10 mM Tris, pH 8.5) for 12 hours. Finally, the omentum was re-coated with AgNO 3 solution (1 mg / ml) for 16 hours to obtain the nanosilver coated, polyethylene glycol (PEG) ester and gelatin hydrogel impregnated polyurethane (NSPU) omentum of the present invention. In the in vivo tests for ventral hernia repair, the PEG side of the omentum was used as the inner side and the gelatin sid...
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