Oudemansiella raphanipes liquid strain culture medium and preparation method thereof
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A technology for liquid strains and culture medium, which is applied to the field of liquid strain culture medium of red soil chicken fir and its preparation, can solve the problems of difficulty in meeting market demands and high prices of wild mushrooms, achieve significant economic and social benefits, and common raw materials , the effect of strong growth
Inactive Publication Date: 2017-01-11
云南福保农业科技开发有限公司
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Red earth chicken fir is the top grade of edible fungi, with tender meat, pleasant appearance, delicious taste, high nutritional and health value, taste, quality and eating method are similar to those of
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Embodiment 1
[0016] 1. Prepare the raw material of the liquid culture medium, its material and weight components are: 50 parts of corn distiller's grain waste liquid, 50 parts of red soil leaching liquid;
[0017] 2, the preparation of red soil leachate, the process method is:
[0018] Take 1 part of red soil raw soil below 20-30 cm below the ground surface, add 20 parts of water, boil for 25 minutes, filter through nine layers of gauze, and the filtrate is the red soil leachate;
[0019] 3. Prepare the liquid culture medium, the process method is:
[0020] Add the required substances according to the requirements of the raw material composition of the liquid culture medium. The red soil extract should be shaken well and added while it is hot. After stirring well, adjust the pH value to 5.0-7.5 with citric acid or slaked lime; put the prepared culture material into In culture bottles or fermenters, sterilize at 121°C for 45 minutes, and make liquid medium after cooling;
[0021] The prep...
Embodiment 2
[0025] 1. Prepare the raw material of the liquid culture medium, its material and weight components are: 90 parts of corn distiller's grain waste liquid, 10 parts of red soil leaching liquid;
[0026] 2, the preparation of red soil leachate, the process method is:
[0027] Take 5 parts of red soil raw soil below 20-30 cm below the ground surface, add 50 parts of water, boil for 20 minutes, filter through nine layers of gauze, and the filtrate is the red soil leachate;
[0028] 3. Prepare the liquid culture medium, the process method is:
[0029] Add the required substances according to the requirements of the raw material composition of the liquid culture medium. The red soil extract should be shaken well and added while it is hot. After stirring well, adjust the pH value to 5.0-7.5 with citric acid or slaked lime; put the prepared culture material into In a culture bottle or a fermenter, sterilize at 126°C for 30 minutes, and make a liquid medium after cooling;
[0030] The...
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Abstract
The invention relates to an Oudemansiella raphanipes liquid strain culture medium and a preparation method thereof. The liquid strain culture medium is prepared by using corn distiller's grain liquor as a major component and adding a small volume of red soil extract to the corn distiller's grain liquor. The corn distiller's grain liquor contains 90% to 96% of water, the rest 4% to 10% of dry matters are rich in carbon source, nitrogen source, vitamins and the like which are essential to the growth and development of edible fungi, and the red soil extract is rich in mineral substances and micro-elements, so that nutrients which are essential to the growth of Oudemansiella raphanipes liquid strains can be provided. According to the Oudemansiella raphanipes liquid strain culture medium, the amount of components of the formula is small, the raw materials are common and are readily available, and the cost is low. The preparation method of the culture medium is simple in operation, so that popularization and application are easy. Through culturing the Oudemansiella raphanipes liquid strains by using the culture medium, the pollution caused by the corn distiller's grain liquor is avoided, the cost is greatly reduced, meanwhile, Oudemansiella raphanipes mycelia are robust in growth, high in kink force, pure white in color and high in growth speed, the mushroom emergence is easy, the quality is good, and the efficiency is high, so that problems in strain supply during the large-scale cultivation and industrialized development of Oudemansiella raphanipes are solved, a scientific and technological basis is laid for the sustainable utilization of precious wild mushroom resources, and the economic and social benefits are remarkable.
Description
technical field [0001] The invention belongs to the technical field of domestication and cultivation of wild edible fungi, in particular to a kind of red soil chicken fir (Ganoderma leucocontextum) Liquid culture medium and preparation method thereof. Background technique [0002] Yunnan Red Soil Gallinarum ( Oudemansiella raphanipes ), Chinese name: oosporus small Oddum mushroom, commonly known as water chicken fir, dew chicken fir, oil chicken fir, long-rooted mushroom, long-rooted money fungus, etc., is a famous wild edible fungus in Yunnan. The fruiting body is medium to large, the mushroom body is large, the stipe is white, thick and long, the surface of the cap is taupe, the cap is thick, and the quality is good. It only grows in high-altitude areas above 1,500 meters above sea level in Yunnan. In summer and autumn, it often grows on the wet woodland with red soil with weak acidity, so it is called red soil chicken fir. It is a medium-low temperature fungus with ...
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