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Method for preparing wild rosa SOD complex enzyme

A production method and compound enzyme technology, applied in the direction of biochemical equipment and methods, enzymes, oxidoreductases, etc., can solve the problems that ordinary people are difficult to use, chemical substance pollution, expensive, etc., to maintain rationality and Effects of naturalness, eradication of pollution, and improvement of production efficiency

Inactive Publication Date: 2017-01-11
武熙熙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Using the above method to extract SOD not only has high cost, poor stability, and is not easy to store. With the spread of international mad cow disease and the prevalence of various blood-borne diseases, the use of SOD extracted from blood has brought many risk factors to people. This process has chemical The potential pollution of the substance, and the price is too expensive for ordinary people to use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of preparation method of wild Rosa roxburghii SOD compound enzyme, its technical feature is that its technological process is:

[0020] (1) After cleaning, cleaning, crushing, pressing and centrifuging the prickly pears, keep the pH value of the original solution at 3.8-5;

[0021] (2) Heat the stock solution to 45°C for ultrafiltration, the molecular weight cut-off is 20,000 Daltons, and the retained solution is set aside; the passing solution is then concentrated by reverse osmosis, the retained solution is set aside, and the passing solution is discarded;

[0022] (3) Warm up the ultrafiltration retentate to 50°C and add 2.8% trehalose to react with stirring for 20 minutes;

[0023] (4) The reverse osmosis concentrate and the reacted ultrafiltration retentate are filtered, and the two liquids are mixed;

[0024] (5) The mixed liquid is vacuum concentrated to a solid content of 30%, and the concentration temperature is 50°C;

[0025] (6) The concentrate is fi...

Embodiment 2

[0027] A kind of preparation method of wild Rosa roxburghii SOD compound enzyme, its technical feature is that its technological process is:

[0028] (1) After cleaning, cleaning, crushing, pressing and centrifuging the prickly pears, keep the pH value of the original solution at 3.8-5;

[0029] (2) Heat the stock solution to 55°C for ultrafiltration, the molecular weight cut-off is 20,000 Daltons, and the retained solution is set aside; the passing solution is then concentrated by reverse osmosis, the retained solution is set aside, and the passing solution is discarded;

[0030] (3) Warm up the ultrafiltration retentate to 60°C and add 3.5% trehalose to react with stirring for 30 minutes;

[0031] (4) The reverse osmosis concentrate and the reacted ultrafiltration retentate are filtered, and the two liquids are mixed;

[0032] (5) The mixed liquid is vacuum concentrated to a solid content of 30%, and the concentration temperature is 60°C;

[0033] (6) The concentrate is fi...

Embodiment 3

[0035] A kind of preparation method of wild Rosa roxburghii SOD compound enzyme, its technical feature is that its technological process is:

[0036] (1) After cleaning, cleaning, crushing, pressing and centrifuging the prickly pears, keep the pH value of the original solution at 3.8-5;

[0037] (2) Heat the stock solution to 50°C for ultrafiltration, the molecular weight cut-off is 20,000 Daltons, and the retained solution is set aside; the passing solution is then concentrated by reverse osmosis, the retained solution is set aside, and the passing solution is discarded;

[0038] (3) Warm up the ultrafiltration retentate to 55°C and add 3% trehalose to react with stirring for 25 minutes;

[0039] (4) The reverse osmosis concentrate and the reacted ultrafiltration retentate are filtered, and the two liquids are mixed;

[0040] (5) The mixed liquid is vacuum concentrated to a solid content of 30%, and the concentration temperature is 55°C;

[0041] (6) The concentrate is fill...

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Abstract

The invention discloses a method for preparing a wild rosa SOD complex enzyme. According to the technical scheme, the method comprises the following steps: cleaning and washing rosa fruits; breaking cell walls with high pressure; preparing SOD-containing stock juice; centrifugally separating at a high speed; performing ultra-filtration; performing reverse osmosis concentration; stabilizing an SOD crude enzyme; concentrating in vacuum; and lyophilizing to obtain the rosa SOD complex enzyme. The method has a reasonable process flow, easily selected processing equipment, an easily controlled production control point and an easily formulated and implemented industrial scheme, and has good application prospects in medicines, health foods and cosmetics.

Description

technical field [0001] The invention relates to the technical field of biological food, in particular to a preparation method of wild Rosa roxburghii SOD compound enzyme. Background technique [0002] SOD is the most popular topic in the international medical circle in the 20th century. A large number of medical studies have proved that SOD is an important human superoxide anion free radical O2- scavenger, which can treat various diseases caused by superoxide anion free radicals produced by human metabolism in clinical practice, such as various inflammations, rheumatism, Rheumatoid causes autoimmune diseases, alcoholic liver poisoning, atherosclerosis, coronary heart disease, acute myocarditis, pulmonary edema, emphysema, Parkinson's disease, acute and chronic renal failure and uremia, diabetes, and is especially found to be effective in preventing cancer Cell proliferation, transfer, anti-radiation, immune enhancement and special effects in anti-aging and beauty. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 武熙熙
Owner 武熙熙
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