Method for identifying cotton seedling-stage salt tolerance

Abstract

The invention provides a method for identifying cotton seedling-stage salt tolerance. A non-invasive microtest technique is utilized to determine the Na<+> ion flow in the cotton seedling hypocotyl and mesophyll tissues under salt stress, and the expression condition of the GhSOS1 gene in the cotton seedling hypocotyl and mesophyll tissues under salt stress is combined to distinguish the cotton germplasm materials with different salt tolerances. After four cotton species with significant differences in salt tolerance are grown in a sandy soil culture medium in an artificial culture box, various physiologic indexes of various species under salt stress are researched, and the non-invasive microtest technique and qRT-PCR (quantitative reverse transcription-polymerase chain reaction) technique are adopted to determine the variations of the Na<+> ion flow and Na<+> transport gene GhSOS1 under salt stress are determined. The results indicate that the cotton species with different salt tolerances under salt stress have obvious differences in the Na<+> absorption dynamic variation trend, and the differences between the Na<+> ion flow and Na<+> equilibrium related gene can be utilized to quickly and accurately identify the salt tolerance of the cotton species. Thus, the method has a certain practical value.

Description

technical field [0001] The invention relates to the fields of electrophysiology, molecular biology and plant genetic breeding, in particular to a method for identifying the salt tolerance of cotton seedlings. Background technique [0002] Saline-alkali land and secondary saline-alkali land have become a worldwide problem and are important factors that limit crop growth and yield. my country is one of the countries with large saline-alkali land in the world. The area of ​​salinized soil accounts for about 1 / 4 of the existing cultivated land in my country, and the area continues to expand, which has seriously threatened my country's food security and ecological environment. Therefore, an accurate understanding of the physiology of plants to salt stress Response has become a growing focus (Sarura et al., 2014). Cotton is one of the crops with strong salt tolerance, and it has gradually become a new dominant crop in saline-alkali land because of its better salt tolerance. At pr...

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Problems solved by technology

There are many identification and evaluation methods for salt tolerance (Liu Guoqiang et al., 1993; Sun Xiaofang et al., 2001; Zhang Guowei et al., 2011; Wang Junjuan et al., 2011), such as germination stage screening method, seedling stage identification method, nutrient solution screening method, field identification method, etc. ; but most of the salt tolerance identification methods are complicated, the measurement period is long, and the difference in the treatment conditions, evaluation indicators and classification standards in the actual utilization process often leads to different resistance levels of the same material, which limits the application in actual production (Zhang Peng et al., 2013; Jiang Jinghan et al., 2013; Peng Zhen et al., 2014; Liu Xue et al., 2015)

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