Compound microorganism preparation for preventing and treating powdery mildew and preparation method thereof

A technology of composite microorganisms and microbial preparations, which is applied in the field of composite microbial preparations for the prevention and treatment of cucumber powdery mildew and its preparation, can solve the problems of easy resistance, limitation, and easy loss of resistance in chemical control, and achieve high efficacy and pertinence The effect of strong and low usage

Inactive Publication Date: 2017-02-01
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the difficulty of breeding for disease resistance and easy loss of resistance; chemical control has the problems of easy generation of drug resistance and pollution to humans and the environment, and its application is limited by conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A compound microbial preparation for preventing and treating powdery mildew, characterized in that the raw material ratio of the compound microbial preparation is: 30 parts of Streptomyces rugosa fermentation broth, 20 parts of Streptomyces phloem fermentation broth, and 30 parts of Bacillus mohaiwei fermentation broth , 30 parts of Bacillus lysinus fermented liquid.

[0032] The preparation method of the composite microbial preparation for preventing and treating powdery mildew comprises the following steps:

[0033] Step 1: Preparation of Streptomyces rugosa fermentation broth

[0034] Take out the preservation tube of Streptomyces rugosa, scratch the plate with Gaoshi No. 1 solid medium for recovery, culture at 30°C for 7 days, pick a single colony under the plate and inoculate it with 150 ml of Gaoshi No. 1 solid medium. In the eggplant bottle, cultivate it in an incubator at 30°C for 6-8 days. After the bacterial lawn is covered with eggplant bottle, a large amoun...

Embodiment 2

[0053] Embodiment 2 Composite microbial preparation antibacterial activity detection test

[0054] The composite microbial preparation prepared in Example 1 was diluted 100 times and 200 times, and the impact of the two microbial preparations on the growth of powdery mildew mycelium was detected by the plate confrontation method. An Oxford cup was placed in the center of the PDA plate, and 100 uL of the microbial preparation dilution was added dropwise, and the cucumber powdery mildew bacterial cake (5 mm in diameter) cultivated for 2 days was placed symmetrically around it. The treatment of dripping water was used as the control, and each treatment was repeated three times. After culturing at a constant temperature of 28°C for 5 days, the inhibition of the growth of mycelia by the microbial preparations was observed, and the diameter of the inhibition zone was measured.

[0055] Test results: After diluting the compound microbial preparation of the present invention 200 time...

Embodiment 3

[0057] Experimental Effects of Compound Microbial Preparations for Controlling Cucumber Powdery Mildew in Greenhouses

[0058] (1) Test treatment:

[0059] Test group: The method for preventing and treating cucumber powdery mildew of the composite microbial preparation prepared in Example 1 is to spray 4L / acre each time on the foliage, and the dilution factor is 150 times, and it is used continuously for 2 times, once every 7 days;

[0060] Chemical agent: 56% azoxystrobin chlorothalonil 600 times solution, used twice continuously;

[0061] Blank control: clear water

[0062] (2) Test method: From January to July 2015, it was carried out in a cucumber greenhouse in Cang County, Cangzhou City, Hebei Province. At the beginning of the experiment, powdery mildew on cucumber leaves moderately occurred in the whole shed. Three rows per plot, four replicates, arranged in random blocks. The composite microbial preparation prepared in Example 1 was inoculated with a knapsack electr...

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Abstract

The invention discloses a compound microorganism preparation for preventing and treating powdery mildew and a preparation method thereof, and particularly discloses a compound microorganism preparation for preventing and treating powdery mildew of cucumbers and a preparation method thereof. Raw materials of the compound microorganism preparation include Streptomyces plicatus fermentation liquor, Streptomyces phaeofaciensMaeda fermentation liquor, Bacillus mojavensis fermentation liquor and Lysinibacillus sphaericus fermentation liquor. The strains of the compound microorganism preparation can generate multiple antibiotics such as pheofacin, amicetin, amicecin B and plicacetin and perform a super-strong effect on pathogenic bacteria of powdery mildew of cucumbers. The four strains used in the compound microorganism preparation can be directly separated from soil, have a rhizosphere growth promoting effect, can reproduce and grow on the surfaces of crops or in plants or in soil, and also can directionally secrete some secondary metabolites towards the rhizosphere of plants, improve nutrient absorption of plants, simulate plant growth and perform a fertilizer effect. The compound microorganism preparation is safe to human and livestock and environmentally friendly.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological control and application of microbial agents, and in particular relates to a compound microbial agent for preventing and treating cucumber powdery mildew and a preparation method thereof. Background technique [0002] Erysiphales are specialized plant pathogenic fungi, belonging to Ascomycotina, Pyrenomycetes, Erysiphales, Erysiphaceae, mostly ectoparasitic, mycelium All or most of them are exposed on the surface of the leaves, stems, shoots, buds, flowers and fruits of the host plant, and produce a large amount of white powdery matter visible to the naked eye consisting of mycelia, conidiophores and conidia. [0003] There are 18 genera and 435 species of powdery mildew in my country, which are various, widely distributed and seriously harmful. After the occurrence of powdery mildew, the growth of the affected part of the host is inhibited, and the greening and yellowing gradually...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/465C12R1/07
CPCA01N63/00A01N63/10C12N1/20
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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