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Procalcitonin immunofluorescence quantitation test strip and preparation method thereof

A procalcitonin, immunofluorescence technology, applied in the field of immunological detection, can solve the problem of high requirements for specialization of venues, equipment and personnel, and achieve the effects of good storage stability, high accuracy and good precision

Active Publication Date: 2017-02-01
ZHONGSHAN CHUANGYI BIOCHEM ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for quantitative detection of PCT mainly include enzyme-linked fluorescence method and electrochemiluminescence method. However, these methods require large-scale instruments and require high professional requirements for venues, equipment and personnel.

Method used

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  • Procalcitonin immunofluorescence quantitation test strip and preparation method thereof
  • Procalcitonin immunofluorescence quantitation test strip and preparation method thereof
  • Procalcitonin immunofluorescence quantitation test strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation process of procalcitonin immunofluorescence quantitative test strip is as follows:

[0025] (1) The preparation of preferred stock solution: the mass concentration of PB is 20mM, the percentage concentration of BSA is 1.8%, the percentage concentration of Tween-80 is 0.5%, the percentage concentration of glucose is 0.5%, the percentage concentration of glycine The percentage concentration of PEG4000 is 1%, the percentage concentration of PEG20000 is 1.5%, and the percentage concentration of Proclin300 is 0.03%. After mixing according to the above formula, filter and sterilize to prepare the storage solution;

[0026] (2) Preparation of the sample pad: soak the glass fiber membrane with the sample pad treatment solution for 10 minutes, place in a drying room at 37°C and 30% humidity, and dry for 3 hours to prepare the sample pad for use;

[0027] (3) Preparation of the bonding pad: Soak the glass cellulose membrane with the bonding pad treatment solution ...

Embodiment approach

[0041] The procalcitonin immunofluorescence quantitative test strip prepared in Example 1 is used to establish a standard curve and detect it. The implementation method is as follows:

[0042](1) Establish a standard curve: mix and dilute to 0.1ng / mL, 0.25ng / mL, 0.5ng / mL, 2ng / mL, 10ng / mL with Roche procalcitonin concentration of 54ng / mL and 0.1ng / mL standard , 20ng / mL, 40ng / mL, 50ng / mL eight concentrations. Add 80 μL of prepared test strips to immunofluorescence analysis for detection, and repeat 3 times for each concentration to obtain the average value. Take the ratio of the T peak area of ​​the detection line and the C peak area of ​​the quality control line as the ordinate, and the theoretical value of the standard product as the abscissa, to obtain the linear regression equation;

[0043] standard curve as figure 1 As shown, the linear relationship y=0.699x+0.0459, R 2 =0.9904, this equation can be used to calculate the content of procalcitonin in the sample, to achiev...

Embodiment 3

[0047] Prepare the control storage solution, the specific formula is: the mass concentration of PB is 50mM, the percentage concentration of BSA is 1%, the percentage concentration of Tween-80 is 1%, the percentage concentration of glucose is 1%, and the percentage concentration of glycine 0.5%, the percentage concentration of PEG4000 is 2%, and the percentage concentration of Proclin300 is 0.3%.

[0048] The preferred storage solution prepared in Example 1 (hereinafter referred to as storage solution 2) is compared with the reference storage solution (hereinafter referred to as storage solution 1) for stabilizing performance and precision, and the implementation method and results are as follows:

[0049] Using storage solution 1 and storage solution 2, the procalcitonin (PCT) primary antibody-fluorescent microspheres labeled with the same process were sprayed and dried, assembled into 40 test strips, put in aluminum foil bags, and sealed with a desiccant. One bag was stored i...

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Abstract

The invention discloses a procalciton inimmunofluorescence quantitation test strip and a preparation method thereof. Compared with a test strip prepared by a common stock solution, the procalcitonin immunofluorescence quantitation test strip prepared by the invention is excellent in storage stability and better in precision, the peaking of a detected sample has a higher signal and a smoother base line, and the accuracy is higher.

Description

technical field [0001] The invention relates to the field of immunological detection, in particular to a procalcitonin immunofluorescence quantitative test strip and a preparation method thereof. Background technique [0002] Procalcitonin (PCT) is a calcitonin propeptide substance without hormonal activity, which is secreted and expressed by inner nerve cells and decomposed into calcitonin (CT), carboxy-terminal peptide and amino-terminal peptide by enzymatic cleavage. The content of PCT in normal human serum is extremely low. When the body has a systemic inflammatory response syndrome, such as bacterial infection, pancreatitis, and burns, all the splicing products of CT propeptide substances in the blood are abnormally elevated, of which PCT is the most important product of. Therefore, the blood PCT level is positively correlated with the severity of infection and injury, and has become an effective indicator for the diagnosis and curative effect monitoring of sepsis, sev...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/543G01N33/577G01N33/68
CPCG01N33/533G01N33/543G01N33/577G01N33/68G01N2333/585G01N2800/26
Inventor 何林辉李瑞机何平
Owner ZHONGSHAN CHUANGYI BIOCHEM ENG
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