Electrochemical immunosensor for detecting alpha fetoprotein and preparation method thereof

An immunosensor, alpha-fetoprotein technology, applied in scientific instruments, instruments, measuring devices, etc., can solve the problems of complex preparation process, time-consuming processing process, long analysis time, etc., and achieve high sensitivity, excellent adsorption, and regulation performance. effect with structure

Inactive Publication Date: 2017-02-08
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The pretreatment process of these methods is time-consuming, the analysis time is long, the requirements for equipment are extremely high, and specialized technicians are required. The most important thing is that these traditional electrochemical analysis processes usually use enzymes as signal probes, but enzymes are used as signal probes. Labeling, the preparation process is complicated, expensive, and the enzyme is easily inactivated, resulting in instability

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  • Electrochemical immunosensor for detecting alpha fetoprotein and preparation method thereof
  • Electrochemical immunosensor for detecting alpha fetoprotein and preparation method thereof
  • Electrochemical immunosensor for detecting alpha fetoprotein and preparation method thereof

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Embodiment 1

[0042] 1. Preparation method of electrochemical immunosensor:

[0043] (1) HAuCl 4 / N-GS / CS composite preparation:

[0044] a. Dissolve 0.7 mg CS in 1 ml of acetic acid solution with a mass fraction of 1%, adjust the pH value to 4.5-5.0 with saturated sodium hydroxide, and stir magnetically for 2 hours to prepare a CS solution;

[0045] b. Dissolve 0.3 mg of N-GS into the above CS solution, and sonicate for 60 minutes to prepare N-GS / CS solution;

[0046] c. Finally, 0.3mgHAuCl 4 Crystals were added to the N-GS / CS solution, and magnetically stirred for 60 minutes to prepare HAuCl 4 / N-GS / CS composite material suspension;

[0047] (2) Preparation of Au / N-GS / CS composite modified electrode:

[0048] Immerse the cleaned glassy carbon electrode into the HAuCl prepared in step (1) 4 / N-GS / CS composite material suspension, use platinum sheet electrode as counter electrode, saturated calomel electrode as reference electrode, under working potential -1.5V, constant potential dep...

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Abstract

The invention discloses an electrochemical immunosensor for detecting alpha fetoprotein and a preparation method thereof. The electrochemical immunosensor comprises a working electrode, a reference electrode and a counter electrode, wherein the working electrode is obtained through firstly modifying a nanogold / nitrogen doped graphene / chitosan composite material on the surface of a substrate electrode, and then respectively fixing an alpha fetoprotein antibody and bovine serum albumin. The preparation method of the electrochemical immunosensor comprises the following steps of (1) preparing a chloroauric acid / nitrogen doped graphene / chitosan composite material; (2) preparing a nanogold / nitrogen doped graphene / chitosan composite material modified electrode;(3) building the electrochemical immunosensor. The electrochemical immunosensor for detecting the alpha fetoprotein provided by the invention has the advantages of high sensitivity, wide select range, high stability, simplicity, quickness and the like.

Description

technical field [0001] The invention relates to the technical fields of biological immunity technology and electrochemical immunosensor, in particular to a preparation method of an electrochemical immunosensor for detecting alpha-fetoprotein. Background technique [0002] Alpha-fetoprotein (AFP) is a glycoprotein mainly derived from embryonic liver cells. The content of AFP in the serum of normal people is less than 20 μg / L, but the content of AFP in patients with liver cancer will increase significantly. Therefore, The detection of AFP is of great significance for the diagnosis of early liver cancer. There are many methods for detecting AFP, including enzyme-linked immunoassay (ELISA), fluorescent immunoassay (FIA), radioimmunoassay (RIA), indirect blood enzyme method and agar double diffusion method. The pretreatment process of these methods is time-consuming, the analysis time is long, the requirements for equipment are extremely high, and specialized technicians are req...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N33/574G01N33/68
CPCG01N27/3278G01N33/57484G01N33/68G01N2333/471
Inventor 卢丽敏徐景坤贾海燕邱新兰
Owner JIANGXI AGRICULTURAL UNIVERSITY
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