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Indoor open alga culture method

A technology of medium and medium components, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve problems such as separation from sterilizing pots and incubators, slow growth of microalgae, etc., and achieve a solution space Limited, solve the effect of low yield, ensure rapid growth and stability

Inactive Publication Date: 2017-02-15
TIANJIN OCEAN PAL CAROL BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the present invention aims to propose a method for cultivating algae indoors, which not only realizes the problem of algae leaving the sterilizer and incubator, solves the problem of slow growth of microalgae, but also ensures that the algae are transferred to the outside Cultivation stability, the present invention is suitable for small and medium-sized enterprises to cultivate algae and stabilize the water environment for aquaculture water bodies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In step 3), inoculate the algae species into the seawater obtained in step 2), the volume content is 11%, in step 4), in the solution prepared in step 3) that has been inoculated with algae, add biotin at a concentration of 0.5g / 50ml 1ml, the growth rate of algae reached a maximum of 91% on the first day, one day earlier than the biotin-free group, and reached 2,000,000 cells / mL on the third day.

[0027] The culture environment of the algae is as follows: the temperature is 27° C.; the light intensity of the incandescent lamp is 800 Lux; the light time is 14 hours (8:00 am to 5:00 pm), and Pyramis sp. is used.

Embodiment 2

[0029] In step 3), the algae species are inoculated into the seawater obtained in step 2), and the volume content is 14%. In step 4), in the solution prepared in step 3) that has been inoculated with algae, the concentration of vitamin B12 added is 0.5g / 50ml , 1ml; can promote the pigment content in each algae cell to increase by 22%.

[0030] The culture environment of the algae is as follows: the temperature is 28° C.; the light intensity of the incandescent lamp is 1500 Lux; the light time is 14 hours (8:00 am to 5:00 pm), and Pyramis sp. is used.

Embodiment 3

[0032] In step 3), the algae species are inoculated into the seawater obtained in step 2), and the volume content is 12%. In step 4), in the solution prepared in step 3) that has been inoculated with algae, 0.4 g of glucose is added to increase the concentration of algae. 1.19 times increase in six days.

[0033] The culture environment of the algae is as follows: the temperature is 26° C.; the light intensity of the incandescent lamp is 1200 Lux; the light time is 14 hours (8:00 am to 5:00 pm), and Pyramis sp. is used.

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Abstract

The invention provides an indoor open alga culture method. Micro-algae are subjected to indoor open culture by using a culture solution, and sodium bicarbonate and nutritional substances are added, wherein the concentration of sodium bicarbonate is 0.0005-0.003g / L, and the culture solution is seawater containing f / 2 culture medium components. According to the method, quick growth of indoor micro-algae is realized, and the micro-algae are enabled to quickly grow indoors through different drug stimulation factors to meet production demands and fertilizer-water work of an outdoor water environment, so that the highest concentration value of the algae is reached in 5 days, and the problems of low yield and slow growth of the micro-algae open in the nature and the long-distance transport expense of a large amount of seawater are solved and settled.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a method for cultivating algae indoors with an open top. Background technique [0002] Microalgae are single-celled or microscopic algae composed of several cells. Rich in protein, vitamins, polyunsaturated fatty acids and other substances. It is generally used as an open feed for aquaculture, especially in the artificial fish fry stage in the early stage of marine fish and shrimp larvae. Generally, there is no pure algae liquid in the market, and it is generally concentrated algae liquid or algae preserved by adding some special substances. During the long transportation journey, the transportation cost and a large amount of labor costs are increased, and if it is not properly preserved, it is easy to cause the algae liquid to deteriorate and cause a waste of resources. Contents of the invention [0003] In view of this, the present invention aims to propose a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 柴英辉孙朦朦于宏周文礼李连星
Owner TIANJIN OCEAN PAL CAROL BIOTECH
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