Genes highly expressed in tongue squamous carcinoma para-carcinoma tissue and applications of genes

A technology of tongue squamous cell carcinoma and genes, which is applied in the application field of genes in the diagnosis of tongue squamous cell carcinoma and the preparation of anti-tongue squamous cell carcinoma preparations, to achieve the effects of improving work efficiency, good stability, and rapid and accurate quantification

Inactive Publication Date: 2017-02-15
BEIJING MEDINTELL BIOMED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular targeted therapy of oral and tongue cancer is still in the experimental research stage, and it is very important to correctly select the specific molecular target

Method used

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  • Genes highly expressed in tongue squamous carcinoma para-carcinoma tissue and applications of genes
  • Genes highly expressed in tongue squamous carcinoma para-carcinoma tissue and applications of genes
  • Genes highly expressed in tongue squamous carcinoma para-carcinoma tissue and applications of genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The collection and preparation of embodiment 1 material

[0037] Tongue squamous cell carcinoma and paracancerous tissue samples were taken from patients with tongue squamous cell carcinoma undergoing radical resection, and normal mucosal tissues were taken from patients with trauma. The specimens were collected within 30 minutes of the surgical procedure, and then collected under the guidance of pathologists. The sample size of each case is about 1.0cm×l.0cm×l.0cm. The cancer tissue is the central area of ​​the tumor when the material is collected; the corresponding paracancerous tissue is the tissue 1-2cm away from the edge of the cancer tissue; the normal mucosa is excluding submucosal Epithelial tissue, the specimens were derived from surgical specimens of oral trauma. All specimens were placed in 1.5ml EP tubes and stored in liquid nitrogen tanks. All specimens were collected from patients who had not undergone any form of anti-tumor treatment and had no history ...

Embodiment 2

[0039] Embodiment 2 database and biological analysis method

[0040] RNA-seq read mapping

[0041] First, the low-quality reads were removed to obtain clean reads, and then TopHat v1.3.11 was used to match the clean reads with the UCSC H. sapiens reference genome (hg19), the human reference genome sequence (hgl9, updated on March 20, 2009) from Downloaded from UCSC database. The Hgl9 genome includes 25 reference sequences (reference assemblies) and 68 variable sequences (alternative assemblies). The pre-built index of H. sapiens UCSC hg19 version is downloaded from the TopHat homepage and used as a reference genome. When using TopHat to match the genome, each read (default to 20) is allowed to have multiple matching sites and a maximum of 2 mismatches . TopHat builds a library of possible splicing sites based on exon regions and GT-AG splicing signals, and maps reads that have not mapped to the genome to the genome based on these splicing site libraries. The system default...

Embodiment 3

[0049] Example 3 Expression of LCE3E gene and GSDMA gene in tongue squamous cell carcinoma tissue and adjacent tissue

[0050] 1. Materials and methods

[0051] 1. Materials

[0052] See Example 1 for the collection criteria. 29 cases of tongue squamous cell carcinoma tissues and paracancerous tissues were collected and grouped and numbered.

[0053] 2. Method

[0054] 2.1 Extraction of RNA

[0055] The RNA was extracted according to Invitrogen's Trizol operating instructions, and stored in a -80°C ultra-low temperature freezer after extraction.

[0056] 2.2 Reverse transcription

[0057] By vazyme (HiScript TM Q RT Super Mix for qPCR) reverse transcription kit instructions. System: 4 μl of 4×gDNA wiper Mix; 2 μl of template RNA; RNase free ddH2O to make up to 20 μl. Gently pipette to mix, 42°C for 2 minutes.

[0058] Reverse transcription reaction system: 16 μl of the reaction solution in step 1 and 4 μl of 5×qRT SuperMix II, gently blow and mix with a pipette, incub...

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Abstract

The invention provides genes highly expressed in the tongue squamous carcinoma para-carcinoma tissue and applications of the genes, and in particular relates to the LCE3E gene and the GSDMA gene and applications of expression products of the LCE3E gene and the GSDMA gene in diagnosing tongue squamous carcinoma and preparing biological medicines for treating tongue squamous carcinoma. According to the invention, based on the high-throughput sequencing and in combination with the molecular biology experiment, the screened molecular diagnosis and treatment markers, namely, the LCE3E gene and the GSDMA gene for tongue squamous carcinoma are verified, therefore, an experimental foundation is provided for the clinical applications of the LCE3E gene and the GSDMA gene in tongue squamous carcinoma, and thus a novel target gene is provided for the early diagnosis and treatment of tongue squamous carcinoma.

Description

technical field [0001] The present invention relates to the field of gene diagnosis and treatment, in particular to a gene highly expressed in adjacent tissues of tongue squamous cell carcinoma and its application, and more specifically to the application of the gene in diagnosing tongue squamous cell carcinoma and preparing anti-tongue squamous cell carcinoma preparations. Background technique [0002] Squamous cell carcinoma of the oral mucosa is the most common oral malignancy and one of the ten most common cancers in the world. In recent years, the incidence of oral cancer in my country has been increasing year by year and tends to be younger. Tongue cancer is one of the common malignant tumors in the oral cavity, and tongue cancer ranks first among oral cancers in my country. Tongue cancer refers to malignant tumors that originate in the front swimming part of the tongue, and more than half of them occur in the edge of the middle 1 / 3 of the tongue. Tongue cancer is ea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574A61K31/7088A61K45/00A61P35/00
CPCC12Q1/6886A61K31/7088A61K45/00C12Q2600/158G01N33/57407
Inventor 李海岩肖枫
Owner BEIJING MEDINTELL BIOMED CO LTD
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