Method for analyzing genetic integrity of soybean germplasm
A completeness and germplasm technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as decline in vitality, improve accuracy and reliability, avoid system errors, ensure accuracy and reliability effect
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Embodiment 1
[0034] Example 1 Obtaining of Soybean Different Vitality Groups
[0035] 1. Test material: The soybean landrace variety Da soybean seeds were used as the test material.
[0036] 2. Test method:
[0037] (1) The seeds of the landrace soybean cultivar Rhubarb soybean with consistent sowing, field management, harvest and maturity are used as test materials, and at least 10,000 seeds are used.
[0038] (2) Before the artificial aging of the seeds, put them into an artificial climate box to balance the water content of the seeds. The balance conditions are that the relative humidity is 45%, the temperature is 25° C., and the time is 15 days, so that the water content of the treated seeds is 7.4% ± 0.1 %, if the water content of the seeds does not reach this standard, continue to balance under the same conditions.
[0039] (3) After the moisture balance of the seeds meets the requirements, they are vacuum-sealed and packaged in aluminum foil bags, and divided into several parts on a...
Embodiment 2
[0042] Example 2 Using Morphological Markers to Analyze the Genetic Integrity of Soybean
[0043] 1. Analyzing samples
[0044] The treatment population of the soybean different vigor that embodiment one obtains, with reference to the standard condition in the GB / T3543.4 crop seed inspection procedure, carries out the germination test, and counts indicators such as germination potential, germination rate, germination index and vigor index (Table 1 ). Soybean parental populations with different vigor were regenerated in the field, and 200 plants were planted in each population, and the individual plants were numbered to obtain their offspring populations. 11 morphological marker traits were surveyed on 30 individual plants of each parent population during the reproductive renewal period.
[0045] After harvesting the parent populations with different vigor, individual plants are threshed to obtain the seeds of the progeny populations, and the individual plants of each populat...
Embodiment 3
[0057] Example 3 Analysis of Soybean Genetic Integrity Using SSR Molecular Markers
[0058] 1. Analyzing samples
[0059] With the analysis sample of embodiment two, different vigor parent populations and their progeny populations are during the field reproduction renewal period, get 96 individual plants (including 30 individual plants in embodiment two), collect young leaves, -80 ℃ Save it for future use. There is also a one-to-one correspondence between the single plant where the progeny collects leaves and its parent.
[0060] 2. Genomic DNA extraction
[0061] SDS method was used to extract the genomic DNA of each population of the above-mentioned parent and offspring. The extraction steps are:
[0062] (1) Put young soybean leaves into 2.0mL centrifuge tubes with tweezers, add a steel ball with a diameter of 5mm in each tube, and proceed to the next step or -80°C for later use;
[0063] (2) Quickly put the centrifuge tube into liquid nitrogen to cool down, put it int...
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