Application of nucleolin to preparation of diagnostic reagent and therapeutic for intestinal cancer
A technology of diagnostic reagents and diagnostic kits, which is applied in the field of therapeutic drugs, preparations or kits for the detection or diagnosis of human intestinal cancer, and can solve the problems of early detection of colorectal cancer, difficulty in early diagnosis, low specificity and sensitivity, etc.
Inactive Publication Date: 2017-02-15
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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Problems solved by technology
In current clinical practice, the more common and routine examination is serum blood examination, commonly used serum tumor markers related to the gut, such as serum carcinoembryonic antigen (CEA), colon cancer-associated antigen (CCA), carbohydrate antigen 19-9 (CA19-9) etc. have low specificity and sensitivity for the diagnosis of colorectal cancer; at the same time, because colorectal cancer may have no obvious clinical symptoms in the early stage, these have caused certain difficulties to the early detection and early diagnosis of colorectal cancer; therefore , looking for effective markers of colorectal cancer, and accurately judging the clinical stage and prognosis of the disease has become a hot spot for researchers in this field
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Embodiment 1
[0032] Example 1 ELISA detection of nucleolin in the plasma samples of patients with intestinal cancer and normal controls
[0033] Reagents: ELISA kits were purchased from Uscn Life Science Company.
[0034] Patients and samples:
[0035] The preoperative blood samples of 30 patients newly diagnosed with colorectal cancer between January 2013 and February 2015 were randomly selected, and the blood samples of 28 healthy patients were selected as controls. All blood sample collection protocols were approved by the Ethics Committee of Huashan Hospital.
[0036] Quantitative determination of nucleolin content in human plasma by double antibody ELISA method:
[0037] Perform corresponding operations according to the kit instructions;
[0038] 1. Specimen Collection and Maintenance
[0039] Centrifuge the patient's blood sample at 1000×g for 20 minutes, take the supernatant (plasma) and store it at -20°C or -80°C;
[0040] 2. Operation steps:
[0041] 1) Preparation of standa...
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The invention relates to multi-functional ribosomal protein nucleolin and application thereof, specifically to application of nucleolin as a molecular marker for the intestinal cancer of human beings and application of nucleolin to preparation of a preparation or kit used for detecting and diagnosing the intestinal cancer of human beings and a therapeutic for the intestinal cancer, belonging to the field of biotechnology. According to the invention, ELISA detection is carried out on both the blood plasma sample of a patient with the intestinal cancer and a normal control blood plasma sample; detection results show that the level of nucleolin in the blood plasma of the patient with the intestinal cancer is substantially higher than the level of nucleolin in the normal control blood plasma sample and that nucleolin is highly expressed in the intestinal cancer and secreted to blood; thus, nucleoli can be used as a noninvasive marker for diagnosis of the intestinal cancer. The invention further provides application of nucleolin to preparation of a diagnostic reagent and kit for the intestinal cancer and a drug with nucleolin as a target and for treatment of the intestinal cancer. The invention provides a novel assistant determination means for diagnosis of a colorectal cancer.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a multifunctional non-ribosomal protein nucleolin and its application, in particular to the application of nucleolin as a human intestinal cancer molecular marker and its preparation or reagent for detecting or diagnosing human intestinal cancer box and its use in therapeutic medicine. [0002] Background technique: [0003] In recent years, with the improvement of people's living standards and changes in eating habits, the incidence and mortality of colorectal cancer are also increasing year by year. Studies have shown that the prognosis of colorectal cancer is closely related to the stage of the disease, early diagnosis and early treatment can significantly improve the prognosis and prolong the survival period. In current clinical practice, the more common and routine examination is serum blood examination, commonly used serum tumor markers related to the gut, such as serum carcinoem...
Claims
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Login to View More IPC IPC(8): G01N33/574
CPCG01N33/57446
Inventor 刘杰张骏郑万威
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV