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Preparation method for phosphate buffer tablet used for elution of IMAC-Cu protein chip

A protein chip and phosphate technology, applied in biological testing, material inspection products, etc., can solve the problems of limited popularization and high price of IMAC-Cu protein chip, and achieve the goal of reducing repetitive labor, facilitating industrial production, and extending shelf life. Effect

Inactive Publication Date: 2017-02-15
TIANJIN GUANGFU TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is especially used in the screening of malignant tumor markers, but because the IMAC-Cu protein chip is expensive and disposable, it limits its popularization and brings a lot of inconvenience

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method (pH is 7.4) of IMAC-Cu protein chip phosphate elution buffer tablet, the steps are as follows:

[0022] (1) Potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride, Tween, and cross-linked amylose in sequence by weight ratio are 27:14.3:500:20:5:33.8. For example, weigh 14.0 g of disodium hydrogen phosphate and 5 g of Tween, grind them first, and mix them evenly. Dry in vacuum and grind finely. Then mix with potassium dihydrogen phosphate 27g, sodium chloride 500g, potassium chloride 20g, cross-linked amylose 33.8g, mix and stir evenly.

[0023] ⑵Compressed into tablets on a tablet machine, the compression pressure is 4 tons, 200 tablets are pressed, each tablet weighs 3g,

[0024] (3) When in use, each tablet is dissolved in 500ml of distilled water, which is the pH7.4 PBS-T elution buffer solution.

Embodiment 2

[0026] A preparation method (pH is 7.2) of IMAC-Cu protein chip phosphate elution buffer tablet, the steps are as follows:

[0027] (1) Potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride, Tween, and cross-linked amylose in the order of weight ratio 20:110:500:20:5:3. For example, weigh 110g of anhydrous disodium hydrogen phosphate and 5g of Tween, grind them first, and mix them evenly. Dry in vacuum and grind finely. Then mix with potassium dihydrogen phosphate 10g, sodium chloride 500g, potassium chloride 20g, cross-linked amylose 20g, mix and stir evenly;

[0028] ⑵Compressed into tablets on a tablet machine, the compression pressure is 3 tons, 200 tablets are pressed, each tablet weighs 3.4g, containing potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride, Tween, crosslinking Amylose;

[0029] (3) When used, each tablet is dissolved in 5010ml distilled water, which is the pH7.2 PBS-...

Embodiment 3

[0031] A preparation method (pH is 7.6) of IMAC-Cu protein chip phosphate elution buffer tablet, the steps are as follows:

[0032] (1) Disodium hydrogen phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride, Tween, and cross-linked amylose, the weight ratio of which is 22:0.7:500:20:5:21.3. Grind first and mix well. Dry in vacuum and grind finely. Then mix with potassium dihydrogen phosphate 1.0g, sodium chloride 500g, potassium chloride 20g, cross-linked amylose 21.3g, mix and stir evenly.

[0033] ⑵Compressed into tablets on a tablet machine, the compression pressure is 2 tons, 200 tablets are pressed, each tablet weighs 3.8g, containing potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride, Tween, crosslinking amylose, than

[0034] (3) When in use, each tablet is dissolved in 500ml of distilled water, which is the pH7.6 PBS-T elution buffer solution.

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PUM

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Abstract

The invention relates to a preparation method for a phosphate buffer tablet used for elution of an IMAC-Cu protein chip. The preparation method is characterized by comprising the following steps: (1) purifying and drying main and accessory solids and weighing particles with particle sizes in a range of 100 to 200 meshes, wherein the main solids are composed of potassium dihydrogen phosphate or sodium dihydrogen phosphate, disodium hydrogen phosphate and Tween and the accessory solids are composed of sodium chloride, potassium chloride and cross-linked amylose; and (2) subjecting a fixed amount of Tween and a fixed amount of anhydrous disodium hydrogen phosphate to grinding, full mixing, vacuum drying and porphyrization at first, and then uniformly mixing the obtained mixture with fixed amounts of potassium dihydrogen phosphate or sodium dihydrogen phosphate, sodium chloride, potassium chloride and cross-linked amylase under stirring. The tablet prepared in the invention is easy to store and keep, convenient to carry and transport, convenient for hermetic package, prolonged in shelf life and reduced in usage and production cost.

Description

technical field [0001] This patent belongs to the technical field of chemical reagents, relates to auxiliary reagents used in biology, medicine and chemical experiments, especially a preparation method of IMAC-Cu protein chip phosphate (PBS-T) elution buffer tablet. Background technique [0002] MAC-Cu protein chip is a new generation of biochip technology. It has great advantages in the fields of disease diagnosis, prevention and new drug development. It is especially used in the screening of malignant tumor markers, but because the IMAC-Cu protein chip is expensive and disposable, it limits its popularization and brings a lot of inconvenience. Contents of the invention [0003] The purpose of the present invention is to provide a kind of preparation method of IMAC-Cu protein chip phosphate elution buffer tablet, adopts tablet technology, prepares the phosphate (PBS-T) elution that is fully suitable for IMAC-Cu protein chip elution requirement Debuffered tablet. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 赵鹏贾伟青
Owner TIANJIN GUANGFU TECH DEV
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