Peony polysaccharide and preparation method and application thereof

A peony and polysaccharide technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., can solve problems such as destruction, cell and tissue damage, and achieve cost reduction, leaching speed, and increase difficulty. Effect

Inactive Publication Date: 2017-03-08
ZHENGZHOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, too much active oxygen free radicals will have a destructive effect, leading to damage to normal cells and tissues of the human body, thus causing various diseases

Method used

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  • Peony polysaccharide and preparation method and application thereof

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preparation example Construction

[0019] The preparation method of peony polysaccharide provided in the embodiment of the present invention uses peony unique to my country as a raw material. Its petals are large, fragrant, and bright in color. It has the reputation of "national beauty and heavenly fragrance", representing luxury and dignity. Specifically, the tree peony in the embodiment of the present invention, for example, can choose one of the leaves or seed coats of "Feng Dan" in Tongling Fenghuang Mountain as raw materials, or both leaves and seed coats can be used as raw materials at the same time. During the preparation process, the raw materials can be pulverized first to increase the contact area between the raw materials and the extractant and increase the leaching speed. Wherein, the crushed granularity of leaves and seed coats can be, for example, 400-600 μm. Preferably, the crushed granularity of leaves and seed coats can also be, for example, 420-580 μm. larger. The raw materials are preferably...

Embodiment 1

[0033] The leaves and seed coats of fresh Fengdan were crushed to particle sizes of 400 μm and 420 μm respectively, and methanol-water solution was used as the extractant, wherein the volume percentage of methanol was 80%. According to the ratio of solid to liquid: 5g: 80mL, the extractant is mixed with the pulverized leaves and seed coats to obtain the extract. The first extract was obtained by leaching in a water bath for 100 min under the condition of 60°C. The first extract was centrifuged at 3000 r / min for 35 min, and the centrifuged supernatant was collected to obtain the first isolate. The first isolate was mixed with a 6% trichloroacetic acid solution by mass percentage at a feeding volume ratio of 1mL:6mL, refrigerated in a refrigerator at 2°C for 48h, then centrifuged at 3000r / min for 30min to remove For the protein part, the supernatant after this centrifugation was collected to obtain a crude polysaccharide solution. Add dehydrated alcohol to the crude polysaccha...

Embodiment 2

[0035] Remove impurities and wash the leaves and seed coats of fresh Fengdan, dry them at 20°C and crush them to particle sizes of 600 μm and 580 μm respectively, using absolute ethanol-water solution as the extractant, wherein the volume percentage of absolute ethanol is 70 %. According to the ratio of solid to liquid: 5g: 400mL, the extractant is mixed with the pulverized leaves to obtain the extract. The first extract was obtained by leaching in a water bath for 30 minutes under the condition of 85°C. The first extract was centrifuged at 5000 r / min for 10 min, and the supernatant was collected to obtain the first isolate. The precipitate remaining after centrifugation was mixed with absolute ethanol-water solution at a material-to-liquid ratio of 5 g: 400 mL, and leached in a water bath for a second time at 85° C. for 30 min to obtain a second extract. The second extract was centrifuged at 5000 r / min for 10 min, and the supernatant was collected to obtain the second isola...

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Abstract

The invention relates to peony polysaccharide and a preparation method and application thereof and belongs to the field of natural product application. The preparation method includes following steps: smashing a peony raw material until grain size of the same is 400-600um; extracting and separating the smashed peony raw material, and collecting supernatant after separation to obtain a coarse extract, wherein extraction and separation are conducted according to a following mode: mixing an extractant with the smashed peony raw material according to a material-liquid ratio of 5-20g:80-400ml, extracting for 30-100min, filtering, and collecting the supernatant; mixing the coarse extract with a protein modifier, standing at 2-8 DEG C for 12-48h, centrifuging, and collecting a supernatant after centrifuging to obtain a coarse polysaccharide solution; adding an anhydrous alcohol solution into the coarse polysaccharide solution to obtain liquid to be concentrated, and concentrating the liquid to obtain the peony polysaccharide. The preparation method is simple and easy to operate. The peony polysaccharide prepared by the method has good effect on scavenging free radicals and can be applied in free radical scavengers.

Description

technical field [0001] The invention relates to the application field of natural products, and in particular to a tree peony polysaccharide and its preparation method and application. Background technique [0002] Free radicals, also known as "free radicals" in chemistry, refer to atoms or groups with unpaired electrons formed by homolytic covalent bonds of molecules of compounds under external conditions such as light and heat. Free radical reactions play an important role in combustion, gas chemistry, polymerization reactions, plasma chemistry, biochemistry, and various other chemical disciplines. Sunlight radiation, air pollution, smoking, pesticides, etc. in the external environment will cause the human body to produce more active oxygen free radicals, causing nucleic acid mutations, which is the root cause of human aging and disease. [0003] Active oxygen free radicals in the body have certain functions, such as immune and signal transduction processes. However, too ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06A61K31/715
CPCA61K31/715C08B37/00C08B37/0003
Inventor 刘瑞霞王国霞陈丽培刘宇邈张净南
Owner ZHENGZHOU NORMAL UNIV
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