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Paper chip gray value recognition method and application thereof

A recognition method and gray value technology, which are used in character and pattern recognition, analysis by chemical reaction of materials, instruments, etc., can solve the problem of difficult to carry, easy to carry, easy to operate, expensive structure of urine analyzer, difficult to analyze and detect. Work completion and other issues to achieve the effect of saving time, reducing costs, and making the production process fast and simple

Inactive Publication Date: 2017-03-08
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing urinalysis techniques require specific analytical instruments for the analysis of specific components such as urine sugar and uric acid, and professional analytical technicians are required to complete a series of operations. The detection process is complex and time-consuming
In addition, specific urine analyzers are expensive and complex in structure, and it is difficult to ensure the completion of analysis and testing in the field and in areas with limited conditions, and it is difficult to meet the requirements of portability, rapid analysis, and easy operation.

Method used

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  • Paper chip gray value recognition method and application thereof
  • Paper chip gray value recognition method and application thereof
  • Paper chip gray value recognition method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Determination of uric acid by APP combined with hand-painted paper chips

[0055] (1) Take an ordinary writing water pen and use a syringe to absorb the ink in the refill.

[0056] (2) Take 0.15g of vacuum silicone grease and add it to 12.5mL n-heptane, ultrasonically dissolve the vacuum silicone grease, then add 0.5g of alkyl ketene dimer (AKD) to the above solution, ultrasonically dissolve, and dissolve the The mixture is used as hydrophobic agent. Use a syringe to draw 0.1 mL of the above hydrophobic agent solution and inject it into a modified pen.

[0057] (3) Use the modified pen above to make a mold with an orifice plate with a diameter of 8mm, draw the pattern directly on Whatman#1 filter paper, then put it in an oven, and dry it at 100°C for 3 minutes to make a paper-based microfluidic chip success, such as figure 2 shown.

[0058] (4) Take uric acid detection reagents uric acid oxidase (80U / mL), horseradish peroxidase (340U / mL), 4-aminoantipyrine (4mM / L),...

Embodiment 2

[0062] APP combined with hand-painted paper chips to measure urine sugar

[0063] (1) Take an ordinary writing water pen and use a syringe to absorb the ink in the refill.

[0064] (2) Take 0.15g of vacuum silicone grease and add it to 12.5mL n-heptane, ultrasonically dissolve the vacuum silicone grease, then add 0.5g of alkyl ketene dimer (AKD) to the above solution, ultrasonically dissolve, and dissolve the The mixture is used as hydrophobic agent. Use a syringe to draw 0.1 mL of the above hydrophobic agent solution and inject it into a modified pen.

[0065] (3) Use the modified pen above to make a mold with an orifice plate with a diameter of 8mm, draw the pattern directly on Whatman#1 filter paper, then put it in an oven, and dry it at 100°C for 3 minutes to make a paper-based microfluidic chip success, such as figure 2 shown.

[0066] (4) Take urine glucose detection reagent glucose oxidase (80U / mL), horseradish peroxidase (340U / mL), KI each 1μL dropwise to the cent...

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Abstract

The invention discloses a paper chip gray value recognition method and an application thereof. Urine sugar and uric acid in urine are mainly used as landmarks; after a drawn paper chip is loaded with a corresponding color reagent, the related contents, such as urine sugar, uric acid and the like, in the urine are detected to obtain corresponding relations between the uric acid and urine sugar concentrations and an RGB value and a gray value; and then related content concentration of the urine is obtained by photographing and analysis through compiled gray value recognition software. The paper chip gray value recognition software provided by the invention has the characteristics of simplicity and rapidness, low cost, high stability, time-saving, convenience and the like, and is particularly suitable for daily personal diagnosis and underdeveloped areas.

Description

technical field [0001] The invention relates to the technical field of a paper-based microfluidic chip and its grayscale recognition. Background technique [0002] The content of uric acid in urine is related to the rate of nucleic acid catabolism in the body, and the increase of its content can be used as an important indicator for the diagnosis of diseases such as gout, chronic leukemia, and renal function decline. The urine sugar in urine is closely related to the blood sugar content, and the detection of the urine sugar concentration has certain reference significance for the monitoring of blood sugar. Most of the existing urine analysis techniques require specific analytical instruments for the analysis of specific components such as urine sugar and uric acid, and professional analytical technicians are required to complete a series of operations. The detection process is complicated and time-consuming. In addition, specific urine analyzers are expensive and complex in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06K9/38G06K9/40G06K9/44G01N21/78
CPCG01N21/78G06V10/34G06V10/28G06V10/30
Inventor 尚晓叶张天越王少男陈诚张敏胡坪
Owner EAST CHINA UNIV OF SCI & TECH
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