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Phlebopus portentosus strain

A technology of porcini and porcini is applied in the field of PP002 strains of porcini pubescens, which can solve the problems of long period of fruiting cultivation and the like, and achieve short fruiting cultivation time, high application value, and fruiting. neat effect

Active Publication Date: 2017-03-15
YUNNAN INST OF TROPICAL CROPS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But its fruiting cultivation period is long

Method used

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  • Phlebopus portentosus strain
  • Phlebopus portentosus strain

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Example 1: ITS sequencing identification

[0019] Extraction and detection of genomic DNA: use the plant genomic DNA extraction kit of OMEGA to extract the total DNA of the sample. Electrophoresis to detect the extraction of genomic DNA: mix the extracted DNA sample with 10×Loading Buffer (bromophenol blue added) at a ratio of 10:1 and load it on a 1% agarose gel, electrophoresis at 150V for 10 minutes, and Detection under UV light.

[0020] PCR amplification of the ITS fragment: PCR amplification of the ITS of strain pp002 was performed using universal primers ITS4 and ITS5. The 50 μL PCR amplification reaction system is: 1 μL of 50 ng / μL template; 25 mM MgCl 2 5 μL; 1 μL of 10 mM dNTPs; 1 μL of 6 μM pre- and post-primers; 0.2 μL of 5 U / μL DNA Taq enzyme; 5 μL of 10 PCR reaction buffer; 35.8 μL of deionized water. The PCR amplification reaction uses touchdown PCR (Touchdown PCR) reaction, and the reaction program is: 95°C pre-denaturation for 5 min, followed by 94°...

Embodiment 2

[0022] Example 2: SSR marker identification

[0023] After screening, five SSR primers in Table 1 were used to identify the strain pp002 and other six Dictyostelium bruceae strains (pp10003, 15010, 15011, ZW1, 16003, 16012). Entrust Kunming Chiqing Biotechnology Co., Ltd. to synthesize primers and add FAM fluorescent labels to the forward primers.

[0024] Table 1. SSR primers

[0025]

[0026] The extraction and detection of genomic DNA, PCR amplification method is the same as embodiment 1. PCR products were genotyped using an ABI3730 sequencer. For the off-machine data of the ABI 3730 sequencer, the software Gene mapper 4.1 was used to analyze the exact position of the data with reference to the core base repeat number of the primer correspondence. NTSYSpc-2.10 software was used to calculate the genetic similarity coefficient, and the unweighted pairwise arithmetic mean method (UPGMA) was used for cluster analysis.

[0027] According to the UPGMA clustering results, t...

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Abstract

The invention relates to a phlebopus portentosus PP002 strain, and belongs to the technical field of edible fungi. The phlebopus portentosus PP002 belongs to boletinellaceae, phlebopus and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on October 13, 2016, the preservation number is CGMCC No.13171, and the address of the preservation unit is the institute of microbiology of Chinese academy of sciences at number 3, number 1 yard, Beichen west road, Chaoyang district, Beijing city. The phlebopus portentosus PP002 strain has the advantages that liquid strain prepared from the strain is golden yellow in liquor color, small and dense in fungus ball and high in spawn running speed and can be used for inoculation after being cultured for 6-7 d; when the liquid strain is used for bag material cultivation, the fruiting culture time is short, order fruiting is achieved, the fruiting body yield is high, large-scale phlebopus portentosus production can be achieved, and a high application value is achieved; a fruiting body has the morphological characteristics that the pileus is brown, the stipe is yellow, the height is 4.5 cm to 6.5 cm, and the base is slightly expanded.

Description

technical field [0001] The invention relates to a Phlebopus portentosus PP002 bacterial strain, which belongs to the technical field of edible fungi. Background technique [0002] Phlebopus portentosus belongs to the family Boletinellaceae, Phlebopus genus. Abroad, it is distributed in tropical regions such as Thailand, Sri Lanka, Vietnam, Indonesia, Brazil, Mexico, Australia, and New Zealand. Domestically, it is mainly distributed in Yunnan, and it is also distributed in Guangxi and Hainan. Boletus dulcis contains 17 kinds of amino acids (including 7 kinds of essential amino acids), low in fat, high in dietary fiber, rich in a variety of essential trace elements (such as iron, zinc, copper, manganese) and high in potassium, low sodium etc. [0003] The strain of Dictyostelium palnus is obtained by natural breeding method, using wild Dictyostelium boletus fruiting bodies to separate, purify, screen for fruiting, and then directly use it for the cultivation of liquid strai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 何明霞曹旸张春霞许欣景刘静高锋王文兵方艺伟杨天伟田耀华王云
Owner YUNNAN INST OF TROPICAL CROPS
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