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Method of inhibiting fusarium to generate deoxynivalenol toxin by procyanidine and application of method

A technology of deoxynivalenol and proanthocyanidins, which is applied in the field of food safety to achieve the effect of ensuring safety and reducing accumulation

Inactive Publication Date: 2017-03-15
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, once the toxins exist in large quantities, it is difficult to completely remove them with existing methods

Method used

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  • Method of inhibiting fusarium to generate deoxynivalenol toxin by procyanidine and application of method
  • Method of inhibiting fusarium to generate deoxynivalenol toxin by procyanidine and application of method
  • Method of inhibiting fusarium to generate deoxynivalenol toxin by procyanidine and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Procyanidin A 2 Preparation isolation and structure identification.

[0027] 1. Material:

[0028] Fungus: F. proliferatum (F. proliferatum), isolated from starfruit, morphologically and molecularly identified, was found to infect a variety of plants.

[0029] Potato dextrose agar medium (PDA): Take 200g of peeled potatoes, cut into small pieces, add 1000mL of water and boil for 30min, filter out the potato pieces, make up the filtrate to 1000mL, add 20g of glucose and 15g of agar, and dissolve it. Sterilize at 121°C for 20 minutes after packaging.

[0030] Czapek’s agar medium (CA): sodium nitrate 3.0g / L, dipotassium hydrogen phosphate 1.0g / L, magnesium sulfate (MgSO 4 ·7H 2 O) 0.5g / L, potassium chloride 0.5g / L, ferrous sulfate 0.01g / L, sucrose 30g / L, agar 20g / L, dissolve the above components in water according to their content, and sterilize at 121°C for 20 minutes.

[0031] Czapek’s broth medium (CB): sodium nitrate 3.0g / L, dipotassium hydrogen phosphate 1.0g / L, ma...

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Abstract

The invention discloses a method of inhibiting fusarium to generate deoxynivalenol (DON) toxin by procyanidine and application of the method. The method and the application thereof have the advantages that the procyanidine is added according to the quantity in the method, toxin yield of the fusarium can be lowered by 30-80%, toxin accumulation can be reduced, and accordingly, food safety is guaranteed.

Description

[0001] Technical field: [0002] The invention belongs to the field of food safety, and specifically relates to a method and application for inhibiting the production of deoxynivalenol toxin by Fusarium by using proanthocyanidins. [0003] Background technique: [0004] Grain and fruits are the main food sources that people depend on for survival. In recent years, with the improvement of logistics fresh-keeping technology, food and fruit have been transported to thousands of households over long distances. However, from the process of harvesting, transportation, shelf sales to consumer logistics, mechanical damage is easily suffered, plus the storage environment The high temperature, high humidity and high carbon dioxide are easy to breed pathogenic bacteria. In addition, food is prone to pathogenic bacteria in the secondary processing process, such as vegetable pickling and fruit juice extraction. In addition to causing food spoilage, these pathogens also produce mycotoxins. Comm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20
Inventor 简琪洁屈红霞李涛涛龚亮周宜洁蒋跃明刘娟吴启贤
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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