Device and method for preparing nanotip transfer pipette

Abstract

The invention discloses a device and a method for preparing a nanotip transfer pipette, and belongs to the fields of nanofabrication. The device comprises a capillary permeability verification device, a capillary drawing system, a capillary etching and a current loop detecting system. The device can be used to prepare a permeable nanotip from a glass capillary or a fused quartz capillary with varied internal and external diameter ratios, and the main breakthrough is that the device is suitable for the capillary with a very small internal and external diameter ratio. The permeable nano transfer pipette tip with the external diameter of the conical tip less than 1 [mu]m is prepared, so that the liquid at picoliter or even femtoliter level can be accurately transferred. The method combines wet etching and current detecting; compared with a traditional preparation method and the conventional other methods, the method has the advantages of simplicity, convenience, high flexibility and high controllability, and moreover, the liquid transferring accuracy is greatly improved; and as the size of the prepared tip is relatively small, the damage to an operation object is greatly reduced.

Description

technical field [0001] The invention relates to a method and system for preparing a nano-tip pipette integrating micro-nano capillary drawing, wet etching and current detection, belonging to the field of nano-processing. Background technique [0002] With the rapid development of the miniaturization of analytical systems and the miniaturization of analytical samples in analytical chemistry, nano-tip pipettes have gradually entered the field of vision of researchers. Manipulation becomes reality. At the same time, nanotip pipettes have also received attention in the biological field, especially in micromanipulation techniques. Micromanipulation technology is now widely used in many important fields of experimental biology research, such as microseparation of cells and chromosomes, nuclear transfer, micromanipulation of embryos, microinjection and microfertilization, etc. During the operation, the damage of the pipette tip to the cells is inevitable, especially for smaller c...

AI Technical Summary

Problems solved by technology

[0005] The manufactured capillary with the outer diameter of the tip at the nanometer level is called a nano-tip pipette. The disadvantages of this production are: the tip size of the capillary is large, and it is impossible to obtain a small or small size; the steps are relatively complicated

On this basis, in order to obtain a smaller-sized tip, some researchers used the method of ion beam bombardment etching to obtain a smaller-sized permeable tip by using the drawn capillary tip, but the cost of this method is relatively high. , most laboratories cannot make

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