Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Perforin chaperonin polypeptide and application thereof

A chaperone and perforin technology, applied in the field of perforin chaperone polypeptides, can solve the problems of immature development of perforin chaperone polypeptides, and achieve the effect of improving survival rate and promoting apoptosis

Inactive Publication Date: 2017-03-15
SUZHOU PULUODA BIOLOGICAL SCI & TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no well-developed perforin chaperone polypeptide available for the treatment of malignant tumors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Effect of perforin chaperone polypeptide on apoptosis of human liver cancer stem cells cultured in vitro.

[0015] The logarithmic growth of human liver cancer stem cells was added to a 96-well culture plate at 1.0×105, and cultured for 24 hours. Different concentrations of the experimental drug perforin chaperone polypeptide (Shanghai Sangong Synthetic) and positive drug were added to the experimental wells and positive drug control wells, respectively. The control drug paclitaxel; the blank group was added the same volume of solvent. Five replicate wells were set up in each well. After culturing for 48 hours, acridine orange (AO)-ethidium bromide (EB) fluorescent double staining method was used; terminal deoxynucleotidyl transferase-mediated DNA fragment end labeling method (TUNEL Method) According to the instructions of TUNEL-POD kit of Boster Bioengineering Company. The results showed that the apoptosis rate of liver cancer stem cells treated with perforin chaperon...

Embodiment 2

[0017] IC50 of perforin chaperone polypeptide on the growth and survival of human liver cancer cells cultured in vitro.

[0018] Using the MTT colorimetric method. The logarithmic growth of human liver cancer cells was added to a 96-well culture plate at 1.0×105 and cultured for 24 hours. Different concentrations of the experimental drug perforin chaperone polypeptide (Shanghai Sangong Synthetic) and positive drug were added to the experimental wells and positive drug control wells respectively. The control drug paclitaxel; the blank group was added the same volume of solvent. Set up five duplicate wells in each hole. After culturing for 48 hours, MTT was added to each hole. After 4 hours of action, DMSO was added, incubated for 30 minutes, and the absorbance A value was measured at 620 nm in a microplate reader. According to the formula, the growth inhibition rate of human liver cancer cells = (1- Absorbance value of the experimental group / absorbance value of the control gro...

Embodiment 3

[0020] IC50 of perforin chaperone polypeptide on the growth and survival of human liver cancer stem cells cultured in vitro.

[0021] Using the MTT colorimetric method. The logarithmic growth of human liver cancer stem cells was added to a 96-well culture plate at 1.0×105, and cultured for 24 hours. Different concentrations of the experimental drug perforin chaperone polypeptide (Shanghai Sangong Synthetic) and positive drug were added to the experimental wells and positive drug control wells, respectively. The control drug paclitaxel; the blank group was added the same volume of solvent. Set up five duplicate wells in each well, culture for 48 hours, add MTT to each well, add DMSO after 4 hours of action, incubate for 30 minutes, measure the absorbance A value at 620nm in a microplate reader, according to the formula human liver cancer stem cell growth inhibition rate = (1-experimental Absorbance value of the group / absorbance value of the control group) × 100%. The calculat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of medicine, and particularly relates to a polypeptide which is capable of promoting activity of perforin and treating malignant tumor. Sequences of the polypeptide are brand new sequences, are capable of inhibiting proliferation activities of a hepatoma carcinoma cell and a liver cancer stem cell simultaneously at the same time, and can promote apoptosis of the liver cancer stem cell. In an in-vivo test, a survival rate of a nude mouse bearing the liver cancer and the tumor is improved, and thus the perforin chaperonin polypeptide has potential new drug development value.

Description

technical field [0001] The invention relates to a perforin chaperone polypeptide and its application, in particular to a polypeptide having the activity of promoting perforin and treating malignant tumors. Background technique [0002] Cellular immunity plays an important role in the anti-tumor effect. Cytotoxic T cells (CTLs) and NK cells are important effector cells of cellular immunity. The main way for CTL and NK cells to kill target cells is to kill target cells through secreted perforin and granzymes. Together, the granzymes programmed cell death. In studies with perforin-deficient mice, it was found that the perforin-mediated target cell lysis pathway plays a more important role in tumor regression. [0003] Perforin (perforin, belonging to the pore-forming protein, PFP) is a glycoprotein with a molecular weight of 67kD that exists in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and NK cells, also known as C9 Related protein or cytolysin (cytolysin). W...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47A61K38/16A61P35/00
CPCA61K38/00C07K14/4705
Inventor 罗瑞雪
Owner SUZHOU PULUODA BIOLOGICAL SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com