Co-dominant SSR markers closely linked to tobacco TMV resistant gene N and application of co-dominant SSR markers

Abstract

The invention discloses co-dominant SSR markers closely linked to tobacco TMV resistant gene N and application of the co-dominant SSR markers. Serial numbers of the co-dominant SSR markers are TM508-007 and TM508-118, and nucleotide sequences of amplified products of the co-dominant SSR markers are shown as SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4 respectively. The application refers to application of the co-dominant SSR markers in detecting whether tobacco genome DNA contains the tobacco TMV resistance gene N or not. The co-dominant SSR markers have the advantages of stability, reliability, simplicity, convenience, quickness and low cost, thereby being applicable to N gene molecular marker assisted selection in tobacco TMV disease resistant breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a co-dominant SSR marker closely linked with tobacco TMV resistance gene N and application thereof. Background technique [0002] Tobacco mosaic is caused by Tobacco Mosaic Virus (TMV), the first single-stranded positive-sense virus identified (Beijerinck MW, Uebereincontagiumvivumfluidumalsursachederfleckenkrankheit der tabaksblatter.VerhKonAkadWetensch, 1898, 5: 3-21) , belonging to the mosaic virus family (Dawson WO and Lehto KM, Regulation of tobamovirus gene expression, Adv. Virus. Res., 1990, 8: 307-342), with infectious activity (Erickson FL, Holzberg S, Calderon-Urrea A, Handley V , Axtell M, Corr C, and Baker B, Thehelicase domain of the TMV replicase proteins induces the N-mediated defense response in tobacco. Plant J., 1999, 18: 67-75). Because the host is mainly Solanaceae crops including tobacco, it seriously affects the yield and quality of tobacco leaves, ...

AI Technical Summary

Problems solved by technology

However, all the above-mentioned molecular markers developed based on the N gene are dominant markers, which have serious defects in the actual tobacco breeding practice: 1) cannot distinguish all genotypes of the N gene in the material, that is, the above-mentioned dominant markers only It can tell whether the material contains the resistance gene N, but cannot further distinguish homozygous resistance (NN) and heterozygous resistance (Nn); In the N gene detection of N gene markers, if no characteristic (target) band appears, it cannot be determined that the test material is susceptible, and there is a high possibility of PCR amplification failure caused by experimental factors; 3) The detection system is cumbersome and has Some markers are designed based on the full-length cDNA of the N gene, so the actual detection involves the extraction of RNA, the synthesis of cDNA, and the PCR amplification of long fragment DNA.

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