Schwanniomyces occidentalis source nucleic acid feed additive and application thereof

A feed additive and yeast technology, applied in the direction of application, animal feed, additional food elements, etc., to achieve the effect of reducing breeding costs, significant economic benefits, and improving animal health

Active Publication Date: 2017-03-22
播恩集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The purpose of the present invention is to provide a yeast-derived nucleic acid feed additive and its application in the preparation of animal breeding feed, which solves the problem of using agricultural and sideli

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation of embodiment 1 bacterial classification

[0044] Source of the strain: the strain of Schwanniomyces occidentalis was purchased from China Food Fermentation Research Institute, China Industrial Microorganism Culture Collection Center, No. 32911.

[0045] (1) Inoculate Schwann yeast on plate medium, culture at 30° C. for 24 hours, and obtain activated cells. Plate medium composition: 3.0g yeast extract, 1.0g malt extract, 3.0g soluble starch, 5.0g peptone, 10.0g glucose, 20.0g agar, 1.0L distilled water, pH6.5. Use after autoclaving at 121°C.

[0046] (2) Seed culture: inoculate the bacteria in step (1) into the seed medium, and culture at 30° C. and 200 rpm for 24 hours to obtain seed liquid. The composition of the seed medium: 3.0g of yeast extract, 1.0g of malt extract, 3.0g of soluble starch, 5.0g of peptone, 10.0g of glucose, 1.0L of distilled water, pH6.5. It is used after autoclaving at 121°C.

[0047] (3) Fermentation culture: inoculate the seed...

Embodiment 2

[0048] DNA and RNA content determination in the wet thalline of embodiment 2

[0049] (1) The DNA extraction method is as follows:

[0050] Reagents: SE buffer (1M sorbitol, 0.1M EDTA, pH7.5, solvent is water); lysozyme (50mg / ml); 20% PVP; proteinase K buffer (10mM Tris, pH7.6, 0.5% SDS , 1 mM EDTA, the solvent is water).

[0051] Dissolve 0.2 g of the wet bacteria obtained in Example 1 in 590 μL of SE buffer and mix well, add 10 μL of lysozyme, react at 37 ° C for 1 hr, centrifuge at 12000 rpm for 8-10 min, collect the precipitate and dissolve it in 100 μL of proteinase K buffer, add Mix well in 0.5 μL proteinase K (1 mg / mL aqueous solution), react at 37°C for 1 hour, add 1.2ml of SDS / NaCl (1% SDS, 100 mM NaCl), 200 μL 20% PVP, mix well, and react at 65°C for 30 minutes. Add an equal volume of saturated phenol / chloroform / isoamyl alcohol mixture, mix well, centrifuge at 12000rpm for 4-5min, and repeat once. Take the supernatant, add ethanol for precipitation, dry, add 1 μL ...

Embodiment 3

[0061] Embodiment 3 preparation of yeast source nucleic acid feed additive

[0062] Composition of solid-state fermentation medium: bran 500g, corn flour 550g, soybean meal 500g, molasses 250g, water 1800g, pH natural. The above-mentioned raw materials can be purchased in the market.

[0063] The fermented liquid containing the wet thalline prepared in Example 1 was inoculated into the solid-state fermentation medium in an amount of 10% (the weight percent of the fermented liquid containing the thalline and the solid substrate), and fermented and cultivated at 30° C. for 48 h. Turn the material once every 12 hours. After the fermentation is over, the fermentation product is steam-dried to a moisture content of ≤11.5%, and then crushed to a fineness of 40-60 mesh to obtain 1756 g of yeast-derived nucleic acid feed additive. Sampling, RNA and DNA were extracted from the ground sample according to the method in Example 2, and the total amount of nucleic acid was calculated to be...

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PUM

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Abstract

The present invention discloses a schwanniomyces occidentalis source nucleic acid feed additive and an application thereof. The additive is prepared by the method: a fermentation liquid obtained by a fermentation culture of schwanniomyces occidentalis is used as an enzyme source, the schwanniomyces occidentalis is inoculated into a solid fermentation medium, the solid fermentation culture is conducted at a condition of 30 DEG C and 200 rpm, after the end of the culture, fermentation products are dried to a moisture content of less than or equal to 12%, and the dried fermentation products are crushed to obtain the schwanniomyces occidentalis source nucleic acid feed additive. The method improves the utilization rate of starch resource and at the same time produces the safe schwanniomyces occidentalis feed additive. The feed additive is rich in amylase, nucleic acids and other substances, can be used for animal breeding, can improve animal health levels, reduces farming costs, and brings significant economic benefits to enterprises.

Description

[0001] (1) Technical field [0002] The invention relates to a feed additive, in particular to a yeast-derived nucleic acid feed additive and its application in preparing animal breeding feed. [0003] (2) Background technology [0004] Nucleic acid is an important biological macromolecule substance, and nucleotide is the basic unit. Nucleotides are involved in many metabolic processes of life activities and play an important role in cell metabolism. Nucleotides are low-molecular-weight compounds that encode genetic information to regulate energy metabolism, transmit cell signals, and serve as coenzymes and other important physiological and biochemical functions. Cell proliferation is essential to an organism and is important for its biological function, and is completely dependent on these nucleotides, since approximately 3 billion nucleotides are required for the division of just one cell. [0005] Nucleic acid plays an extremely important role in the growth and health of a...

Claims

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Application Information

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IPC IPC(8): A23K20/153C12N1/16C12R1/645
CPCC12N1/16
Inventor 朱廷恒汪坤王渭霞吕梦甜潘虹章亭洲
Owner 播恩集团股份有限公司
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