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Preparation method of radix aconiti lateralis and rhizoma pinelliae alkaloid extract

An extract and alkaloid technology, which is applied in the field of preparation of aconite and pinellia alkaloid extracts, can solve problems such as inconvenient application of aconite pinellia decoction, and achieve the effects of good extraction and enrichment effect, improved quality, and good quality.

Inactive Publication Date: 2017-03-22
成都景睿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to overcome the inconvenient deficiency of Fuzi Banxia Decoction in the prior art, and provide a preparation method of Fuzi Banxia alkaloid extract

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Prepare an aqueous extract

[0026] Take 1 kg of raw aconite, slice it, soak it in 10 liters of water, decoct it for 3 hours, and filter to collect the filtrate. Add 10 liters of water to the filter residue, continue decocting for 2 hours, and collect the filtrate by filtration. Add 3 liters of clean water to the filter residue, continue to boil on high heat for 30 minutes, collect the filtrate by filtration, and combine the above filtrates. Concentrate to 5ml of water extract corresponding to 1g of raw medicinal material.

[0027] Purification of aqueous extract to obtain aconitum pinellia alkaloid extract

[0028] Put the above-mentioned water extract on a D101 macroporous adsorption resin column according to the conventional method, wash it with 3 liters of water to remove impurities, and then use 70 (v)% (the same below) hydrochloric acid ethanol-water solution with pH=4 to elute. Collect the ethanol eluate, add appropriate amount of sodium bicarbonate to adju...

Embodiment 2

[0030] Purification of aqueous extract to obtain aconitum pinellia alkaloid extract

[0031] The aconite aqueous solution prepared by the method of Example 1 was put on a HPD100 type macroporous adsorption resin column, washed with 4 liters of water to remove impurities, and then eluted with 65 (v)% hydrochloric acid ethanol-water solution with pH=5, collected Add appropriate amount of sodium hydroxide to the ethanol eluate to adjust the pH to neutral, recover ethanol under reduced pressure, concentrate and dry to obtain the total alkaloid extract of Pinellia aconitifolia. It has been detected that the transfer rate of aconitine (Mesoaconitine, Aconitine, Hypoaconitine) in Aconitum to Pinellia aconitum alkaloid extract is greater than 71.4%.

Embodiment 3

[0033] Prepare an aqueous extract

[0034] Take 2 kg of raw aconite, slice it, soak it in 15 liters of clean water, decoct it on warm fire for 2 hours, filter and collect the filtrate. Add 12 liters of water to the filter residue, continue decocting for 2 hours, and collect the filtrate by filtration. Add 8 liters of water to the filter residue, continue to decoct with warm fire for 1 hour, collect the filtrate by filtration, and combine the above filtrates. Concentrate to 5ml of water extract corresponding to 1g of raw medicinal material.

[0035] Purification of aqueous extract to obtain aconitum pinellia alkaloid extract

[0036] Put the above-mentioned water extract on a D101 macroporous adsorption resin column according to the conventional method, first wash it with 5 liters of water to remove impurities, and then wash it with 70 (v)% hydrochloric acid ethanol-water solution with pH=4.5, collect the ethanol for elution solution, adding an appropriate amount of sodiu...

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Abstract

The invention discloses a preparation method of radix aconiti lateralis and rhizoma pinelliae alkaloid extract. The preparation method comprises the following steps: (1) adsorbing aqueous solution of the extract of radix aconiti lateralis and rhizoma pinelliae by adopting a macroporous adsorption resin; (2) carrying out washing by adopting water or an alkaline aqueous solution with the pH being 7-9.5; and (3) carrying out eluting by adopting an alcohol solution with the pH being smaller than or equal to 4.5, collecting the eluate, removing the solvent, and carrying out concentrating till the dry state is achieved, thus obtaining the radix aconiti lateralis and rhizoma pinelliae alkaloid extract. Compared with the traditional extraction technology, the preparation method of the radix aconiti lateralis and rhizoma pinelliae alkaloid extract has the advantages that the extraction enrichment effect of alkaloid is good, the impurities can be completely removed, meanwhile, the alkaloid component in radix aconiti lateralis and rhizoma pinelliae is not obviously lost or damage, the recovery rate is high, and the quality of the prepared alkaloid extract is good.

Description

technical field [0001] The invention relates to aconite extract, in particular to a preparation method of aconite and pinellia alkaloid extract, belonging to the research field of traditional Chinese medicine extracts. Background technique [0002] Aconite and pinellia are common ingredients in traditional Chinese medicine. Fuzi Banxia Decoction, a traditional Chinese medicine prescription, is used for symptoms such as stomach deficiency and cold phlegm attack, head dizziness, dizziness and vomiting. [0003] Aconitum, the processed product of the root of Aconitum carmichaeli Debx. It is excavated from late June to early August, and the mother root, fibrous root and sediment are removed. It is commonly called "Ni Fuzi" and processed into the following varieties. [0004] Pinellia, the dry tuber of Pinellia ternata (Thunb.) Breit. Excavated in summer and autumn, washed, removed the skin and fibrous roots, and dried in the sun. [0005] The research and application of tradi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8888A61P1/00A61P1/08
CPCA61K36/714A61K36/8888A61K2236/331A61K2236/51A61K2236/55
Inventor 胡燕
Owner 成都景睿生物科技有限公司
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