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High-added-value treating method for waste culture liquid of Dunaliella salina

A treatment method and high value-added technology, applied in microorganism-based methods, filtration treatment, water/sewage treatment, etc., can solve problems such as the reduction of the activity of salina polysaccharides, and achieve the effect of reducing emissions, reducing costs, and improving economic benefits

Active Publication Date: 2017-03-22
山东纳美达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these two documents are based on the polysaccharide extraction method of salina cells (dry powder or salina mud), which are suitable for experiments with higher polysaccharide content, and can only be used for reference in the removal of protein and polysaccharide glycerol in salina waste liquid, and High temperature will lead to the decrease of salina polysaccharide activity

Method used

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  • High-added-value treating method for waste culture liquid of Dunaliella salina
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  • High-added-value treating method for waste culture liquid of Dunaliella salina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Effect of Different Sand Filtration Conditions on Filtration Results

[0054] Sand filter setting parameters First, the selection range of sand particle size is 0.5 ~ 1.5, 1.5 ~ 3, 3 ~ 4.5mm, the sand filter thickness is 0.7m, and the sand filter filtrate of 1.5 ~ 3.5mm is determined to be free of solid suspended particles; the flow rate setting range is 200~500L / h, 500~1000L / h 1000~1500L / h, 1500~2000L / h, it proves that the filtrate of 1000~1500L / h is clear, there is no solid precipitation after standing and the efficiency is high;

Embodiment 2

[0056] Effect of different filter column conditions on filtration results

[0057] (1) Set the filler ratio to 8 / 10, set the flow rate to 500L / h, 600L / h, 700L / h, 800L / h, 900L / h, 1000L / h, and treat for 5 hours. The experimental results show that the flow rate is 700L / h At ~900L / h, the adsorption rates of salina exopolysaccharide and glycerol are about 89% and 58%, respectively, which is beneficial to the adsorption efficiency;

[0058] (2) Set different processing time to determine the processing capacity: 5~7h, 7~9h, 9~11h, 11~13h, when the processing time is 9h~11h, the adsorption rates of salina polysaccharide and glycerin are about 86% and 55% respectively ;

Embodiment 3

[0060] Determination of different elution conditions

[0061] ⑴ After being treated with macroporous resin X-5 adsorption column for 10 hours, elution is carried out, and the elution conditions are set at a sodium chloride concentration of 0.5% to 1%, 1% to 2%, 2% to 3%, 3% to 4 %, at a temperature of 25°C, elute with an eluent with a pH value of 7 at a flow rate of 1500 L / h for 3 hours. The results showed that when the salt concentration was 2% to 3%, the elution rate was over 70%; the yields of salina exopolysaccharide and glycerin were 9.4% and 7.6%, respectively.

[0062] (2) Set the pH range of the eluent as pH=5~6, 6~7, 7~8, 8~9, the elution salt concentration is 2.5%, the temperature is 25°C, and the flow rate is 1500L / h for 3 hours. When the pH value was 8, the elution rate was above 76%; the recoveries of salina exopolysaccharide and glycerol were 9.6% and 7.7%, respectively.

[0063] (3) The elution temperature setting range is 20-25°C, 25-30°C, 30-35°C, 35-40°C, 4...

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Abstract

The invention relates to a high-added-value treating method for waste culture liquid of Dunaliella salina. The method comprises the following steps: subjecting waste culture liquid of Dunaliella salina obtained after collection of cells of Dunaliella salina to sand filtration so as to remove insoluble impurities; then carrying out adsorption with negative X-5 resin; and carrying out elution to recover Dunaliella salina polysaccharide and glycerin in the waste culture liquid of Dunaliella salina, supplementing nutrients into a filtrate and then using the filtrate as a Dunaliella salina medium again. Experimental results show that the method recovers 8.59 mg of Dunaliella salina polysaccharide and 2.87 mg of glycerin per L of the waste culture liquid and has recovery efficiency of 11.6 and 7.8%, respectively. The method enables the waste culture liquid of Dunaliella salina to be reused three times and can retain the biomass of Dunaliella salina, the content of carotenoids and the like; the method increases added value, saves the water resource and salt and is easy to implement; and the key points are that the water resource is save, additional benefits are produced, and a good method is provided for development of Dunaliella salina and natural carotenoid resources.

Description

technical field [0001] The invention relates to a method for treating and recycling waste liquid from cultivation of algal plants, in particular to a method for extracting beneficial polysaccharides and glycerol from high-density culture waste liquid of salina and a method for reusing the waste liquid. Background technique [0002] Dunaliella salina, also known as salina, is a cell-wallless, flagellated, unicellular algae and is one of the most salt-tolerant eukaryotes. Due to the relatively high salt concentration required for salina cultivation, the residual salt concentration in the culture waste liquid is relatively high and cannot be discharged at will. Moreover, the waste liquid contains more high-value-added products such as exopolysaccharides and glycerin. These metabolites have relatively high economic value and are directly discharged, resulting in waste of resources. Therefore, it is of great significance to explore a high value-added treatment method for salina ...

Claims

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Application Information

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IPC IPC(8): C02F9/02C12N1/12C12R1/89
CPCC02F1/001C02F1/285C12N1/12
Inventor 吕和鑫王世磊贾士儒韩培培
Owner 山东纳美达生物科技有限公司
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