Furanone compound, preparation method and application thereof
A compound, the technology of furanone, which is applied in the field of furanone compounds, can solve problems such as drugs that have not yet been seen
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Embodiment 2
[0025] The preparation method of the furanone compound shown in formula I specifically comprises the steps:
[0026] (1) Fermentation production
[0027] Aspergillus versicolor ( Aspergillus versicolor DJ013) was revived by streaking, inoculated on PDA solid slant medium, and cultured in a 28°C incubator for 5 days; all the colonies obtained from slant culture were inoculated into solid medium (dissolve 80g rice in 120ml seawater, soak overnight, Autoclave at 121°C for 20 minutes), then ferment at 28°C for 40 days to obtain the fermented product;
[0028] (2) Obtaining the extract
[0029] Soak the above-mentioned fermented product in 4L of methanol for 3 times, then concentrate and evaporate the methanol extract to dryness, redissolve it in 1L of water, and then extract it with 1L of ethyl acetate for 3 times, combine the extracts obtained from the three extractions and concentrate under reduced pressure to remove ethyl acetate ester to obtain crude extract;
[0030] (3) ...
Embodiment 3
[0037] In vitro antifungal activity test (96-well plate antibacterial method)
[0038] (1) Experimental samples
[0039] Preparation of the test sample solution: the test sample is the pure compound I isolated and purified in Example 1 above, and an appropriate amount of sample is accurately weighed, and prepared into a solution of the required concentration with DMSO for testing the activity. The indicator bacteria used in this experiment were Cryptococcus neoformans and Candida albicans.
[0040] (2) Experimental method
[0041] 96-well plate antifungal test method: The test compound was serially diluted in 20% DMSO / saline, and transferred 10 µL to a 96-well flat-bottomed microplate. Under aerobic conditions, the two bacterial indicator strains were cultured on Sabouraud agar medium (SDA) at 30° C. for 16-20 hours. A series of different concentrations of compounds were added to RPMI1640 medium, and fungal strains were inoculated. Cryptococcus neoformans was cultured at 35...
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